Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3514

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3512 and Pf1N1B4_3513 are separated by 11 nucleotidesPf1N1B4_3513 and Pf1N1B4_3514 are separated by 247 nucleotidesPf1N1B4_3514 and Pf1N1B4_3515 overlap by 4 nucleotidesPf1N1B4_3515 and Pf1N1B4_3516 are separated by 1 nucleotides Pf1N1B4_3512 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASI (EC 2.3.1.41), at 3,918,597 to 3,919,817 _3512 Pf1N1B4_3513 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 3,919,829 to 3,920,344 _3513 Pf1N1B4_3514 - Sensor histidine kinase/response regulator, at 3,920,592 to 3,922,493 _3514 Pf1N1B4_3515 - FIG00953467: hypothetical protein, at 3,922,490 to 3,923,422 _3515 Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547 _3516 Position (kb) 3920 3921 3922 3923Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3920.500 kb on - strandat 3920.537 kb on - strandat 3920.537 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.594 kb on - strandat 3920.841 kb on + strand, within Pf1N1B4_3514at 3920.842 kb on - strand, within Pf1N1B4_3514at 3920.842 kb on - strand, within Pf1N1B4_3514at 3920.842 kb on - strand, within Pf1N1B4_3514at 3920.876 kb on + strand, within Pf1N1B4_3514at 3920.877 kb on - strand, within Pf1N1B4_3514at 3920.877 kb on - strand, within Pf1N1B4_3514at 3920.877 kb on - strand, within Pf1N1B4_3514at 3920.975 kb on + strand, within Pf1N1B4_3514at 3921.011 kb on + strand, within Pf1N1B4_3514at 3921.011 kb on + strand, within Pf1N1B4_3514at 3921.011 kb on + strand, within Pf1N1B4_3514at 3921.011 kb on + strand, within Pf1N1B4_3514at 3921.012 kb on - strand, within Pf1N1B4_3514at 3921.012 kb on - strand, within Pf1N1B4_3514at 3921.012 kb on - strand, within Pf1N1B4_3514at 3921.012 kb on - strand, within Pf1N1B4_3514at 3921.012 kb on - strand, within Pf1N1B4_3514at 3921.013 kb on + strand, within Pf1N1B4_3514at 3921.013 kb on + strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.014 kb on - strand, within Pf1N1B4_3514at 3921.152 kb on + strand, within Pf1N1B4_3514at 3921.153 kb on - strandat 3921.415 kb on - strand, within Pf1N1B4_3514at 3921.488 kb on + strandat 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.488 kb on + strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.489 kb on - strand, within Pf1N1B4_3514at 3921.862 kb on + strand, within Pf1N1B4_3514at 3921.863 kb on - strand, within Pf1N1B4_3514at 3921.953 kb on + strand, within Pf1N1B4_3514at 3921.954 kb on - strand, within Pf1N1B4_3514at 3921.954 kb on - strand, within Pf1N1B4_3514at 3921.954 kb on - strand, within Pf1N1B4_3514at 3922.057 kb on + strand, within Pf1N1B4_3514at 3922.057 kb on + strand, within Pf1N1B4_3514at 3922.057 kb on + strand, within Pf1N1B4_3514at 3922.057 kb on + strand, within Pf1N1B4_3514at 3922.057 kb on + strand, within Pf1N1B4_3514at 3922.058 kb on - strand, within Pf1N1B4_3514at 3922.058 kb on - strand, within Pf1N1B4_3514at 3922.058 kb on - strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.198 kb on + strand, within Pf1N1B4_3514at 3922.199 kb on - strand, within Pf1N1B4_3514at 3922.199 kb on - strand, within Pf1N1B4_3514at 3922.199 kb on - strand, within Pf1N1B4_3514at 3922.199 kb on - strand, within Pf1N1B4_3514at 3922.199 kb on - strand, within Pf1N1B4_3514at 3922.227 kb on + strand, within Pf1N1B4_3514at 3922.227 kb on + strandat 3922.227 kb on + strand, within Pf1N1B4_3514at 3922.228 kb on - strand, within Pf1N1B4_3514at 3922.242 kb on + strand, within Pf1N1B4_3514at 3922.242 kb on + strand, within Pf1N1B4_3514at 3922.243 kb on - strand, within Pf1N1B4_3514at 3922.397 kb on + strandat 3922.398 kb on - strandat 3922.463 kb on + strandat 3922.552 kb on + strandat 3922.583 kb on + strandat 3922.583 kb on + strandat 3922.584 kb on - strand, within Pf1N1B4_3515at 3922.584 kb on - strand, within Pf1N1B4_3515at 3922.683 kb on + strand, within Pf1N1B4_3515at 3922.683 kb on + strand, within Pf1N1B4_3515at 3922.683 kb on + strand, within Pf1N1B4_3515at 3922.683 kb on + strand, within Pf1N1B4_3515at 3922.683 kb on + strand, within Pf1N1B4_3515at 3922.684 kb on - strand, within Pf1N1B4_3515at 3922.684 kb on - strand, within Pf1N1B4_3515at 3922.684 kb on - strand, within Pf1N1B4_3515at 3922.687 kb on + strand, within Pf1N1B4_3515at 3922.752 kb on + strand, within Pf1N1B4_3515at 3922.752 kb on + strand, within Pf1N1B4_3515at 3922.752 kb on + strand, within Pf1N1B4_3515at 3922.752 kb on + strand, within Pf1N1B4_3515at 3922.752 kb on + strand, within Pf1N1B4_3515at 3922.791 kb on + strand, within Pf1N1B4_3515at 3922.792 kb on - strand, within Pf1N1B4_3515at 3923.165 kb on + strand, within Pf1N1B4_3515at 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.425 kb on + strandat 3923.426 kb on - strandat 3923.426 kb on - strandat 3923.426 kb on - strandat 3923.426 kb on - strandat 3923.449 kb on + strandat 3923.449 kb on + strandat 3923.449 kb on + strandat 3923.449 kb on + strandat 3923.450 kb on - strandat 3923.470 kb on + strandat 3923.470 kb on + strandat 3923.470 kb on + strandat 3923.470 kb on + strandat 3923.470 kb on + strandat 3923.471 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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3,920,500 - -2.1
3,920,537 - -1.5
3,920,537 - -1.5
3,920,594 - -2.1
3,920,594 - +0.0
3,920,594 - -2.4
3,920,594 - -2.2
3,920,594 - +0.0
3,920,594 - +0.0
3,920,594 - +0.0
3,920,594 - -1.2
3,920,841 + Pf1N1B4_3514 0.13 +0.0
3,920,842 - Pf1N1B4_3514 0.13 +0.0
3,920,842 - Pf1N1B4_3514 0.13 -0.7
3,920,842 - Pf1N1B4_3514 0.13 -0.0
3,920,876 + Pf1N1B4_3514 0.15 +0.0
3,920,877 - Pf1N1B4_3514 0.15 +0.3
3,920,877 - Pf1N1B4_3514 0.15 +0.0
3,920,877 - Pf1N1B4_3514 0.15 +0.0
3,920,975 + Pf1N1B4_3514 0.20 -1.7
3,921,011 + Pf1N1B4_3514 0.22 +0.0
3,921,011 + Pf1N1B4_3514 0.22 +0.1
3,921,011 + Pf1N1B4_3514 0.22 -1.3
3,921,011 + Pf1N1B4_3514 0.22 +0.0
3,921,012 - Pf1N1B4_3514 0.22 -2.1
3,921,012 - Pf1N1B4_3514 0.22 -2.1
3,921,012 - Pf1N1B4_3514 0.22 -2.1
3,921,012 - Pf1N1B4_3514 0.22 -1.4
3,921,012 - Pf1N1B4_3514 0.22 -1.5
3,921,013 + Pf1N1B4_3514 0.22 +0.0
3,921,013 + Pf1N1B4_3514 0.22 -0.1
3,921,014 - Pf1N1B4_3514 0.22 -0.2
3,921,014 - Pf1N1B4_3514 0.22 +0.0
3,921,014 - Pf1N1B4_3514 0.22 -3.0
3,921,014 - Pf1N1B4_3514 0.22 -0.8
3,921,014 - Pf1N1B4_3514 0.22 -1.6
3,921,014 - Pf1N1B4_3514 0.22 +0.0
3,921,014 - Pf1N1B4_3514 0.22 -0.7
3,921,014 - Pf1N1B4_3514 0.22 -0.3
3,921,152 + Pf1N1B4_3514 0.29 +0.0
3,921,153 - -0.4
3,921,415 - Pf1N1B4_3514 0.43 +0.0
3,921,488 + -0.9
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 -0.4
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 +0.0
3,921,488 + Pf1N1B4_3514 0.47 -1.1
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,489 - Pf1N1B4_3514 0.47 -0.6
3,921,489 - Pf1N1B4_3514 0.47 -0.7
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,489 - Pf1N1B4_3514 0.47 -0.4
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,489 - Pf1N1B4_3514 0.47 +0.0
3,921,862 + Pf1N1B4_3514 0.67 +0.0
3,921,863 - Pf1N1B4_3514 0.67 -0.1
3,921,953 + Pf1N1B4_3514 0.72 +0.1
3,921,954 - Pf1N1B4_3514 0.72 +0.0
3,921,954 - Pf1N1B4_3514 0.72 -0.6
3,921,954 - Pf1N1B4_3514 0.72 +0.0
3,922,057 + Pf1N1B4_3514 0.77 +0.0
3,922,057 + Pf1N1B4_3514 0.77 +0.3
3,922,057 + Pf1N1B4_3514 0.77 -2.1
3,922,057 + Pf1N1B4_3514 0.77 +0.0
3,922,057 + Pf1N1B4_3514 0.77 -0.2
3,922,058 - Pf1N1B4_3514 0.77 +0.0
3,922,058 - Pf1N1B4_3514 0.77 +0.6
3,922,058 - Pf1N1B4_3514 0.77 +0.0
3,922,198 + Pf1N1B4_3514 0.84 -0.6
3,922,198 + Pf1N1B4_3514 0.84 -1.3
3,922,198 + Pf1N1B4_3514 0.84 -0.8
3,922,198 + Pf1N1B4_3514 0.84 -0.7
3,922,198 + Pf1N1B4_3514 0.84 +0.0
3,922,198 + Pf1N1B4_3514 0.84 +0.1
3,922,198 + Pf1N1B4_3514 0.84 +0.0
3,922,198 + Pf1N1B4_3514 0.84 -0.4
3,922,198 + Pf1N1B4_3514 0.84 +0.0
3,922,199 - Pf1N1B4_3514 0.84 +0.0
3,922,199 - Pf1N1B4_3514 0.84 +0.0
3,922,199 - Pf1N1B4_3514 0.84 +0.0
3,922,199 - Pf1N1B4_3514 0.84 +0.0
3,922,199 - Pf1N1B4_3514 0.84 +0.0
3,922,227 + Pf1N1B4_3514 0.86 -0.6
3,922,227 + +0.0
3,922,227 + Pf1N1B4_3514 0.86 -1.1
3,922,228 - Pf1N1B4_3514 0.86 +0.0
3,922,242 + Pf1N1B4_3514 0.87 +0.0
3,922,242 + Pf1N1B4_3514 0.87 -0.1
3,922,243 - Pf1N1B4_3514 0.87 -0.5
3,922,397 + +0.0
3,922,398 - +0.0
3,922,463 + +0.0
3,922,552 + -2.5
3,922,583 + +0.0
3,922,583 + +0.0
3,922,584 - Pf1N1B4_3515 0.10 -3.3
3,922,584 - Pf1N1B4_3515 0.10 +0.0
3,922,683 + Pf1N1B4_3515 0.21 -1.2
3,922,683 + Pf1N1B4_3515 0.21 -2.8
3,922,683 + Pf1N1B4_3515 0.21 +0.0
3,922,683 + Pf1N1B4_3515 0.21 +0.0
3,922,683 + Pf1N1B4_3515 0.21 +0.0
3,922,684 - Pf1N1B4_3515 0.21 +0.0
3,922,684 - Pf1N1B4_3515 0.21 -4.1
3,922,684 - Pf1N1B4_3515 0.21 +0.0
3,922,687 + Pf1N1B4_3515 0.21 +0.0
3,922,752 + Pf1N1B4_3515 0.28 -1.1
3,922,752 + Pf1N1B4_3515 0.28 -0.8
3,922,752 + Pf1N1B4_3515 0.28 -1.2
3,922,752 + Pf1N1B4_3515 0.28 +0.0
3,922,752 + Pf1N1B4_3515 0.28 +0.0
3,922,791 + Pf1N1B4_3515 0.32 +0.0
3,922,792 - Pf1N1B4_3515 0.32 -2.7
3,923,165 + Pf1N1B4_3515 0.72 -2.1
3,923,425 + +0.0
3,923,425 + +0.0
3,923,425 + +0.0
3,923,425 + +0.0
3,923,425 + +0.0
3,923,425 + -2.5
3,923,425 + -1.4
3,923,425 + +0.2
3,923,426 - -1.8
3,923,426 - -2.4
3,923,426 - +0.0
3,923,426 - +0.0
3,923,449 + +0.0
3,923,449 + +0.0
3,923,449 + -0.6
3,923,449 + +0.0
3,923,450 - +0.0
3,923,470 + +0.0
3,923,470 + -0.3
3,923,470 + +0.0
3,923,470 + -0.6
3,923,470 + +0.0
3,923,471 - -0.4

Or see this region's nucleotide sequence