Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3389 and Pf1N1B4_3390 are separated by 261 nucleotides Pf1N1B4_3390 and Pf1N1B4_3391 overlap by 7 nucleotides Pf1N1B4_3391 and Pf1N1B4_3392 are separated by 231 nucleotides
Pf1N1B4_3389 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 3,782,867 to 3,783,988
_3389
Pf1N1B4_3390 - RNA polymerase sigma factor RpoE, at 3,784,250 to 3,784,864
_3390
Pf1N1B4_3391 - FIG00955837: hypothetical protein, at 3,784,858 to 3,785,094
_3391
Pf1N1B4_3392 - Pyrophosphate-specific outer membrane porin OprO, at 3,785,326 to 3,786,669
_3392
Position (kb)
3784
3785
3786 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3784.078 kb on - strand at 3784.086 kb on + strand at 3784.086 kb on + strand at 3784.162 kb on + strand at 3784.163 kb on - strand at 3784.163 kb on - strand at 3784.175 kb on + strand at 3784.175 kb on + strand at 3784.272 kb on + strand at 3784.331 kb on + strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.359 kb on - strand, within Pf1N1B4_3390 at 3784.359 kb on - strand, within Pf1N1B4_3390 at 3784.897 kb on + strand, within Pf1N1B4_3391 at 3784.897 kb on + strand, within Pf1N1B4_3391 at 3785.033 kb on + strand at 3785.093 kb on - strand at 3785.129 kb on - strand at 3785.129 kb on - strand at 3785.129 kb on - strand at 3785.297 kb on + strand at 3785.297 kb on + strand at 3785.298 kb on - strand at 3785.429 kb on + strand at 3785.512 kb on + strand, within Pf1N1B4_3392 at 3785.513 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.567 kb on - strand, within Pf1N1B4_3392 at 3785.567 kb on - strand, within Pf1N1B4_3392 at 3785.567 kb on - strand at 3785.605 kb on + strand at 3785.605 kb on + strand, within Pf1N1B4_3392 at 3785.605 kb on + strand, within Pf1N1B4_3392 at 3785.606 kb on - strand, within Pf1N1B4_3392 at 3785.606 kb on - strand, within Pf1N1B4_3392 at 3785.620 kb on + strand, within Pf1N1B4_3392 at 3785.621 kb on - strand at 3785.621 kb on - strand at 3785.632 kb on + strand, within Pf1N1B4_3392 at 3785.632 kb on + strand, within Pf1N1B4_3392 at 3785.671 kb on + strand, within Pf1N1B4_3392 at 3785.671 kb on + strand, within Pf1N1B4_3392 at 3785.695 kb on + strand, within Pf1N1B4_3392 at 3785.696 kb on - strand, within Pf1N1B4_3392 at 3785.807 kb on - strand, within Pf1N1B4_3392 at 3785.807 kb on - strand, within Pf1N1B4_3392 at 3785.884 kb on + strand, within Pf1N1B4_3392 at 3785.885 kb on - strand, within Pf1N1B4_3392 at 3785.885 kb on - strand, within Pf1N1B4_3392
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 3,784,078 - -0.0 3,784,086 + -1.0 3,784,086 + -0.0 3,784,162 + -2.6 3,784,163 - -0.0 3,784,163 - -0.0 3,784,175 + -0.0 3,784,175 + -2.1 3,784,272 + -0.0 3,784,331 + Pf1N1B4_3390 0.13 -0.0 3,784,332 - Pf1N1B4_3390 0.13 -2.1 3,784,332 - Pf1N1B4_3390 0.13 -0.0 3,784,332 - Pf1N1B4_3390 0.13 -0.0 3,784,359 - Pf1N1B4_3390 0.18 -0.0 3,784,359 - Pf1N1B4_3390 0.18 -2.4 3,784,897 + Pf1N1B4_3391 0.16 -4.8 3,784,897 + Pf1N1B4_3391 0.16 -0.0 3,785,033 + -0.9 3,785,093 - -0.0 3,785,129 - -1.3 3,785,129 - -1.7 3,785,129 - -0.0 3,785,297 + -0.0 3,785,297 + -2.3 3,785,298 - -0.0 3,785,429 + -1.4 3,785,512 + Pf1N1B4_3392 0.14 -0.0 3,785,513 - Pf1N1B4_3392 0.14 -0.0 3,785,534 - Pf1N1B4_3392 0.15 -0.0 3,785,534 - Pf1N1B4_3392 0.15 -0.0 3,785,534 - Pf1N1B4_3392 0.15 -0.0 3,785,534 - Pf1N1B4_3392 0.15 -0.0 3,785,566 + Pf1N1B4_3392 0.18 -2.6 3,785,566 + Pf1N1B4_3392 0.18 -0.0 3,785,566 + Pf1N1B4_3392 0.18 -0.0 3,785,566 + Pf1N1B4_3392 0.18 -0.0 3,785,566 + Pf1N1B4_3392 0.18 -0.0 3,785,567 - Pf1N1B4_3392 0.18 -2.5 3,785,567 - Pf1N1B4_3392 0.18 -3.9 3,785,567 - -0.0 3,785,605 + -3.2 3,785,605 + Pf1N1B4_3392 0.21 -0.0 3,785,605 + Pf1N1B4_3392 0.21 -4.5 3,785,606 - Pf1N1B4_3392 0.21 -0.0 3,785,606 - Pf1N1B4_3392 0.21 -2.9 3,785,620 + Pf1N1B4_3392 0.22 -0.0 3,785,621 - -0.0 3,785,621 - -0.0 3,785,632 + Pf1N1B4_3392 0.23 -0.0 3,785,632 + Pf1N1B4_3392 0.23 -0.0 3,785,671 + Pf1N1B4_3392 0.26 -0.0 3,785,671 + Pf1N1B4_3392 0.26 -0.0 3,785,695 + Pf1N1B4_3392 0.27 -0.0 3,785,696 - Pf1N1B4_3392 0.28 -0.0 3,785,807 - Pf1N1B4_3392 0.36 -0.0 3,785,807 - Pf1N1B4_3392 0.36 -4.7 3,785,884 + Pf1N1B4_3392 0.42 -2.0 3,785,885 - Pf1N1B4_3392 0.42 -0.0 3,785,885 - Pf1N1B4_3392 0.42 -0.0
Or see this region's nucleotide sequence