Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3278 and Pf1N1B4_3279 are separated by 56 nucleotides Pf1N1B4_3279 and Pf1N1B4_3280 are separated by 324 nucleotides Pf1N1B4_3280 and Pf1N1B4_3281 are separated by 202 nucleotides Pf1N1B4_3281 and Pf1N1B4_3282 are separated by 126 nucleotides
Pf1N1B4_3278 - putative exported protein, at 3,666,118 to 3,666,621
_3278
Pf1N1B4_3279 - putative membrane protein, at 3,666,678 to 3,667,076
_3279
Pf1N1B4_3280 - Prophage Lp2 protein 6, at 3,667,401 to 3,668,486
_3280
Pf1N1B4_3281 - AidA, at 3,668,689 to 3,669,228
_3281
Pf1N1B4_3282 - AidA, at 3,669,355 to 3,669,858
_3282
Position (kb)
3667
3668
3669 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3666.460 kb on + strand, within Pf1N1B4_3278 at 3666.461 kb on - strand, within Pf1N1B4_3278 at 3666.461 kb on - strand, within Pf1N1B4_3278 at 3666.461 kb on - strand, within Pf1N1B4_3278 at 3666.461 kb on - strand, within Pf1N1B4_3278 at 3666.752 kb on - strand, within Pf1N1B4_3279 at 3666.954 kb on - strand, within Pf1N1B4_3279 at 3666.996 kb on - strand, within Pf1N1B4_3279 at 3667.092 kb on - strand at 3667.145 kb on - strand at 3667.211 kb on + strand at 3667.211 kb on + strand at 3667.212 kb on - strand at 3667.212 kb on - strand at 3667.212 kb on - strand at 3667.212 kb on - strand at 3667.212 kb on - strand at 3667.228 kb on + strand at 3667.229 kb on - strand at 3667.229 kb on - strand at 3667.276 kb on - strand at 3667.276 kb on - strand at 3667.333 kb on + strand at 3667.388 kb on + strand at 3667.388 kb on + strand at 3667.524 kb on + strand at 3667.627 kb on + strand, within Pf1N1B4_3280 at 3667.628 kb on - strand, within Pf1N1B4_3280 at 3667.698 kb on + strand, within Pf1N1B4_3280 at 3667.698 kb on + strand, within Pf1N1B4_3280 at 3667.749 kb on + strand, within Pf1N1B4_3280 at 3667.749 kb on + strand, within Pf1N1B4_3280 at 3667.751 kb on + strand, within Pf1N1B4_3280 at 3667.751 kb on + strand, within Pf1N1B4_3280 at 3667.751 kb on + strand, within Pf1N1B4_3280 at 3667.751 kb on + strand, within Pf1N1B4_3280 at 3667.751 kb on + strand, within Pf1N1B4_3280 at 3667.752 kb on - strand, within Pf1N1B4_3280 at 3667.833 kb on + strand, within Pf1N1B4_3280 at 3667.833 kb on + strand, within Pf1N1B4_3280 at 3667.833 kb on + strand, within Pf1N1B4_3280 at 3667.858 kb on + strand, within Pf1N1B4_3280 at 3667.914 kb on + strand, within Pf1N1B4_3280 at 3667.914 kb on + strand, within Pf1N1B4_3280 at 3667.915 kb on - strand, within Pf1N1B4_3280 at 3667.998 kb on + strand, within Pf1N1B4_3280 at 3667.998 kb on + strand at 3667.999 kb on - strand, within Pf1N1B4_3280 at 3668.011 kb on + strand, within Pf1N1B4_3280 at 3668.105 kb on + strand at 3668.105 kb on + strand, within Pf1N1B4_3280 at 3668.106 kb on - strand, within Pf1N1B4_3280 at 3668.195 kb on + strand, within Pf1N1B4_3280 at 3668.195 kb on + strand, within Pf1N1B4_3280 at 3668.196 kb on - strand, within Pf1N1B4_3280 at 3668.270 kb on + strand, within Pf1N1B4_3280 at 3668.301 kb on + strand, within Pf1N1B4_3280 at 3668.302 kb on - strand, within Pf1N1B4_3280 at 3668.302 kb on - strand, within Pf1N1B4_3280 at 3668.373 kb on + strand, within Pf1N1B4_3280 at 3668.373 kb on + strand, within Pf1N1B4_3280 at 3668.373 kb on + strand at 3668.373 kb on + strand, within Pf1N1B4_3280 at 3668.373 kb on + strand, within Pf1N1B4_3280 at 3668.373 kb on + strand, within Pf1N1B4_3280 at 3668.374 kb on - strand, within Pf1N1B4_3280 at 3668.439 kb on + strand at 3668.445 kb on + strand at 3668.445 kb on + strand at 3668.478 kb on + strand at 3668.478 kb on + strand at 3668.478 kb on + strand at 3668.478 kb on + strand at 3668.479 kb on - strand at 3668.490 kb on - strand at 3668.490 kb on - strand at 3668.490 kb on - strand at 3668.498 kb on + strand at 3668.499 kb on - strand at 3668.586 kb on + strand at 3668.617 kb on + strand at 3668.640 kb on - strand at 3668.653 kb on + strand at 3668.653 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.682 kb on + strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.683 kb on - strand at 3668.686 kb on + strand at 3668.687 kb on - strand at 3668.687 kb on - strand at 3668.687 kb on - strand at 3668.726 kb on + strand at 3668.726 kb on + strand at 3668.727 kb on - strand at 3668.727 kb on - strand at 3668.727 kb on - strand at 3668.814 kb on - strand, within Pf1N1B4_3281 at 3668.947 kb on + strand, within Pf1N1B4_3281 at 3668.948 kb on - strand, within Pf1N1B4_3281 at 3668.955 kb on + strand, within Pf1N1B4_3281 at 3668.955 kb on + strand, within Pf1N1B4_3281 at 3668.955 kb on + strand, within Pf1N1B4_3281 at 3668.955 kb on + strand, within Pf1N1B4_3281 at 3668.956 kb on - strand, within Pf1N1B4_3281 at 3668.956 kb on - strand, within Pf1N1B4_3281 at 3668.956 kb on - strand, within Pf1N1B4_3281 at 3668.956 kb on - strand, within Pf1N1B4_3281 at 3668.956 kb on - strand, within Pf1N1B4_3281 at 3669.066 kb on + strand, within Pf1N1B4_3281 at 3669.178 kb on - strand at 3669.192 kb on + strand at 3669.193 kb on - strand at 3669.210 kb on + strand at 3669.210 kb on + strand at 3669.211 kb on - strand at 3669.361 kb on - strand at 3669.361 kb on - strand at 3669.440 kb on + strand, within Pf1N1B4_3282 at 3669.440 kb on + strand, within Pf1N1B4_3282 at 3669.440 kb on + strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.441 kb on - strand, within Pf1N1B4_3282 at 3669.480 kb on - strand, within Pf1N1B4_3282
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 3,666,460 + Pf1N1B4_3278 0.68 -0.0 3,666,461 - Pf1N1B4_3278 0.68 -2.4 3,666,461 - Pf1N1B4_3278 0.68 -0.0 3,666,461 - Pf1N1B4_3278 0.68 -3.6 3,666,461 - Pf1N1B4_3278 0.68 -0.0 3,666,752 - Pf1N1B4_3279 0.19 -0.0 3,666,954 - Pf1N1B4_3279 0.69 -1.7 3,666,996 - Pf1N1B4_3279 0.80 -2.6 3,667,092 - -0.0 3,667,145 - -3.1 3,667,211 + -0.0 3,667,211 + -2.4 3,667,212 - -0.0 3,667,212 - -0.0 3,667,212 - -0.0 3,667,212 - -2.0 3,667,212 - -0.0 3,667,228 + -0.0 3,667,229 - -0.0 3,667,229 - -0.8 3,667,276 - -1.7 3,667,276 - -1.8 3,667,333 + -2.2 3,667,388 + -0.0 3,667,388 + -1.5 3,667,524 + -0.0 3,667,627 + Pf1N1B4_3280 0.21 -0.8 3,667,628 - Pf1N1B4_3280 0.21 -2.8 3,667,698 + Pf1N1B4_3280 0.27 -0.0 3,667,698 + Pf1N1B4_3280 0.27 -0.0 3,667,749 + Pf1N1B4_3280 0.32 -3.0 3,667,749 + Pf1N1B4_3280 0.32 -0.0 3,667,751 + Pf1N1B4_3280 0.32 -0.0 3,667,751 + Pf1N1B4_3280 0.32 -2.0 3,667,751 + Pf1N1B4_3280 0.32 -0.0 3,667,751 + Pf1N1B4_3280 0.32 -1.4 3,667,751 + Pf1N1B4_3280 0.32 -0.0 3,667,752 - Pf1N1B4_3280 0.32 -0.0 3,667,833 + Pf1N1B4_3280 0.40 -0.0 3,667,833 + Pf1N1B4_3280 0.40 -1.8 3,667,833 + Pf1N1B4_3280 0.40 -0.0 3,667,858 + Pf1N1B4_3280 0.42 -1.9 3,667,914 + Pf1N1B4_3280 0.47 -0.0 3,667,914 + Pf1N1B4_3280 0.47 -0.0 3,667,915 - Pf1N1B4_3280 0.47 -0.0 3,667,998 + Pf1N1B4_3280 0.55 -4.3 3,667,998 + -2.2 3,667,999 - Pf1N1B4_3280 0.55 -0.0 3,668,011 + Pf1N1B4_3280 0.56 -2.5 3,668,105 + -0.0 3,668,105 + Pf1N1B4_3280 0.65 -1.8 3,668,106 - Pf1N1B4_3280 0.65 -0.0 3,668,195 + Pf1N1B4_3280 0.73 -0.0 3,668,195 + Pf1N1B4_3280 0.73 -0.0 3,668,196 - Pf1N1B4_3280 0.73 -0.0 3,668,270 + Pf1N1B4_3280 0.80 -0.0 3,668,301 + Pf1N1B4_3280 0.83 -0.0 3,668,302 - Pf1N1B4_3280 0.83 -0.0 3,668,302 - Pf1N1B4_3280 0.83 -0.0 3,668,373 + Pf1N1B4_3280 0.90 -0.0 3,668,373 + Pf1N1B4_3280 0.90 -2.1 3,668,373 + -0.0 3,668,373 + Pf1N1B4_3280 0.90 -0.0 3,668,373 + Pf1N1B4_3280 0.90 -0.0 3,668,373 + Pf1N1B4_3280 0.90 -0.0 3,668,374 - Pf1N1B4_3280 0.90 -2.5 3,668,439 + -0.0 3,668,445 + -0.0 3,668,445 + -3.1 3,668,478 + -0.0 3,668,478 + -3.2 3,668,478 + -0.0 3,668,478 + -1.4 3,668,479 - -0.3 3,668,490 - -4.7 3,668,490 - -0.5 3,668,490 - -3.7 3,668,498 + -2.1 3,668,499 - -1.8 3,668,586 + -0.0 3,668,617 + -0.0 3,668,640 - -3.8 3,668,653 + -0.0 3,668,653 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -0.0 3,668,682 + -3.6 3,668,683 - -0.0 3,668,683 - -1.5 3,668,683 - -1.0 3,668,683 - -0.0 3,668,683 - -0.0 3,668,683 - -0.0 3,668,683 - -0.0 3,668,683 - -0.0 3,668,686 + -0.0 3,668,687 - -1.0 3,668,687 - -3.3 3,668,687 - -0.0 3,668,726 + -4.6 3,668,726 + -0.0 3,668,727 - -0.0 3,668,727 - -0.0 3,668,727 - -0.0 3,668,814 - Pf1N1B4_3281 0.23 -3.7 3,668,947 + Pf1N1B4_3281 0.48 -0.0 3,668,948 - Pf1N1B4_3281 0.48 -0.0 3,668,955 + Pf1N1B4_3281 0.49 -0.0 3,668,955 + Pf1N1B4_3281 0.49 -4.2 3,668,955 + Pf1N1B4_3281 0.49 -3.8 3,668,955 + Pf1N1B4_3281 0.49 -3.4 3,668,956 - Pf1N1B4_3281 0.49 -3.2 3,668,956 - Pf1N1B4_3281 0.49 -0.0 3,668,956 - Pf1N1B4_3281 0.49 -3.1 3,668,956 - Pf1N1B4_3281 0.49 -0.0 3,668,956 - Pf1N1B4_3281 0.49 -4.1 3,669,066 + Pf1N1B4_3281 0.70 -2.8 3,669,178 - -3.6 3,669,192 + -4.1 3,669,193 - -5.3 3,669,210 + -3.0 3,669,210 + -3.9 3,669,211 - -0.0 3,669,361 - -0.0 3,669,361 - -0.0 3,669,440 + Pf1N1B4_3282 0.17 -0.0 3,669,440 + Pf1N1B4_3282 0.17 -2.7 3,669,440 + Pf1N1B4_3282 0.17 -0.0 3,669,441 - Pf1N1B4_3282 0.17 -0.0 3,669,441 - Pf1N1B4_3282 0.17 -4.1 3,669,441 - Pf1N1B4_3282 0.17 -0.0 3,669,441 - Pf1N1B4_3282 0.17 -0.0 3,669,441 - Pf1N1B4_3282 0.17 -0.0 3,669,441 - Pf1N1B4_3282 0.17 -0.0 3,669,480 - Pf1N1B4_3282 0.25 -0.0
Or see this region's nucleotide sequence