Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2308 and Pf1N1B4_2309 are separated by 30 nucleotides Pf1N1B4_2309 and Pf1N1B4_2310 are separated by 54 nucleotides Pf1N1B4_2310 and Pf1N1B4_2311 are separated by 114 nucleotides Pf1N1B4_2311 and Pf1N1B4_2312 are separated by 148 nucleotides
Pf1N1B4_2308 - Predicted signal transduction protein, at 2,629,479 to 2,630,345
_2308
Pf1N1B4_2309 - Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23), at 2,630,376 to 2,631,188
_2309
Pf1N1B4_2310 - Cation/multidrug efflux pump, at 2,631,243 to 2,631,566
_2310
Pf1N1B4_2311 - 4Fe-4S ferredoxin, iron-sulfur binding, at 2,631,681 to 2,631,932
_2311
Pf1N1B4_2312 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 2,632,081 to 2,632,560
_2312
Position (kb)
2630
2631
2632 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2629.447 kb on + strand at 2629.448 kb on - strand at 2629.448 kb on - strand at 2629.448 kb on - strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.675 kb on - strand, within Pf1N1B4_2308 at 2629.675 kb on - strand, within Pf1N1B4_2308 at 2629.685 kb on + strand, within Pf1N1B4_2308 at 2629.685 kb on + strand, within Pf1N1B4_2308 at 2629.686 kb on - strand, within Pf1N1B4_2308 at 2629.797 kb on - strand, within Pf1N1B4_2308 at 2629.797 kb on - strand, within Pf1N1B4_2308 at 2629.890 kb on + strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.974 kb on + strand, within Pf1N1B4_2308 at 2629.975 kb on - strand, within Pf1N1B4_2308 at 2629.975 kb on - strand, within Pf1N1B4_2308 at 2630.026 kb on - strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.277 kb on + strand at 2630.277 kb on + strand at 2630.277 kb on + strand at 2630.278 kb on - strand at 2630.550 kb on + strand, within Pf1N1B4_2309 at 2630.550 kb on + strand, within Pf1N1B4_2309 at 2630.550 kb on + strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.551 kb on - strand, within Pf1N1B4_2309 at 2630.692 kb on - strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.789 kb on - strand, within Pf1N1B4_2309 at 2630.789 kb on - strand at 2630.789 kb on - strand, within Pf1N1B4_2309 at 2630.839 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.890 kb on - strand, within Pf1N1B4_2309 at 2630.961 kb on + strand, within Pf1N1B4_2309 at 2630.962 kb on - strand, within Pf1N1B4_2309 at 2631.063 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.087 kb on + strand at 2631.087 kb on + strand, within Pf1N1B4_2309 at 2631.196 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.205 kb on + strand at 2631.205 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.599 kb on + strand at 2631.600 kb on - strand at 2631.600 kb on - strand at 2631.689 kb on + strand at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.936 kb on - strand at 2631.969 kb on - strand at 2632.056 kb on - strand at 2632.082 kb on + strand at 2632.082 kb on + strand at 2632.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 2,629,447 + -0.0 2,629,448 - -3.5 2,629,448 - -0.0 2,629,448 - -0.0 2,629,474 + -0.0 2,629,474 + -2.0 2,629,474 + -0.0 2,629,474 + -0.0 2,629,474 + -0.0 2,629,474 + -0.0 2,629,474 + -2.0 2,629,475 - -0.0 2,629,475 - -0.0 2,629,475 - -2.0 2,629,475 - -0.0 2,629,475 - -0.0 2,629,475 - -1.9 2,629,475 - -0.0 2,629,475 - -0.0 2,629,478 + -0.0 2,629,478 + -0.0 2,629,478 + -0.0 2,629,478 + -0.0 2,629,478 + -1.6 2,629,478 + -0.0 2,629,478 + -3.3 2,629,478 + -2.9 2,629,478 + -0.0 2,629,478 + -3.0 2,629,478 + -0.0 2,629,478 + -1.6 2,629,478 + -0.0 2,629,479 - -1.3 2,629,479 - -2.9 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.9 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -1.7 2,629,479 - -0.0 2,629,479 - -2.5 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -2.6 2,629,479 - -0.0 2,629,479 - -0.0 2,629,479 - -2.1 2,629,578 + Pf1N1B4_2308 0.11 -1.3 2,629,578 + Pf1N1B4_2308 0.11 -1.9 2,629,578 + Pf1N1B4_2308 0.11 -2.9 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -2.2 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -3.2 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -0.1 2,629,579 - Pf1N1B4_2308 0.12 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -2.1 2,629,579 - Pf1N1B4_2308 0.12 -1.8 2,629,579 - Pf1N1B4_2308 0.12 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -0.0 2,629,579 - Pf1N1B4_2308 0.12 -2.5 2,629,675 - Pf1N1B4_2308 0.23 -0.0 2,629,675 - Pf1N1B4_2308 0.23 -0.0 2,629,685 + Pf1N1B4_2308 0.24 -0.0 2,629,685 + Pf1N1B4_2308 0.24 -0.0 2,629,686 - Pf1N1B4_2308 0.24 -0.0 2,629,797 - Pf1N1B4_2308 0.37 -0.0 2,629,797 - Pf1N1B4_2308 0.37 -3.1 2,629,890 + Pf1N1B4_2308 0.47 -0.0 2,629,891 - Pf1N1B4_2308 0.48 -0.0 2,629,891 - Pf1N1B4_2308 0.48 -2.9 2,629,891 - Pf1N1B4_2308 0.48 -1.3 2,629,950 + Pf1N1B4_2308 0.54 -0.0 2,629,950 + Pf1N1B4_2308 0.54 -0.0 2,629,950 + Pf1N1B4_2308 0.54 -1.5 2,629,950 + Pf1N1B4_2308 0.54 -0.0 2,629,951 - Pf1N1B4_2308 0.54 -2.6 2,629,951 - Pf1N1B4_2308 0.54 -2.5 2,629,951 - Pf1N1B4_2308 0.54 -0.0 2,629,974 + Pf1N1B4_2308 0.57 -0.0 2,629,975 - Pf1N1B4_2308 0.57 -0.0 2,629,975 - Pf1N1B4_2308 0.57 -2.9 2,630,026 - Pf1N1B4_2308 0.63 -0.0 2,630,055 + Pf1N1B4_2308 0.66 -3.5 2,630,055 + Pf1N1B4_2308 0.66 -2.6 2,630,055 + Pf1N1B4_2308 0.66 -0.0 2,630,055 + Pf1N1B4_2308 0.66 -0.0 2,630,055 + Pf1N1B4_2308 0.66 -0.0 2,630,055 + Pf1N1B4_2308 0.66 -0.0 2,630,056 - Pf1N1B4_2308 0.67 -0.0 2,630,056 - Pf1N1B4_2308 0.67 -0.0 2,630,056 - Pf1N1B4_2308 0.67 -1.2 2,630,056 - Pf1N1B4_2308 0.67 -4.3 2,630,056 - Pf1N1B4_2308 0.67 -1.6 2,630,056 - Pf1N1B4_2308 0.67 -1.5 2,630,056 - Pf1N1B4_2308 0.67 -0.0 2,630,277 + -0.0 2,630,277 + -2.3 2,630,277 + -1.8 2,630,278 - -0.0 2,630,550 + Pf1N1B4_2309 0.21 -1.5 2,630,550 + Pf1N1B4_2309 0.21 -0.0 2,630,550 + Pf1N1B4_2309 0.21 -2.3 2,630,551 - Pf1N1B4_2309 0.22 -0.0 2,630,551 - Pf1N1B4_2309 0.22 -1.6 2,630,551 - Pf1N1B4_2309 0.22 -0.0 2,630,551 - Pf1N1B4_2309 0.22 -0.0 2,630,551 - Pf1N1B4_2309 0.22 -0.0 2,630,551 - Pf1N1B4_2309 0.22 -2.3 2,630,692 - Pf1N1B4_2309 0.39 -0.0 2,630,788 + Pf1N1B4_2309 0.51 -1.3 2,630,788 + Pf1N1B4_2309 0.51 -0.0 2,630,788 + Pf1N1B4_2309 0.51 -0.0 2,630,788 + Pf1N1B4_2309 0.51 -0.0 2,630,788 + Pf1N1B4_2309 0.51 -0.0 2,630,788 + Pf1N1B4_2309 0.51 -2.3 2,630,789 - Pf1N1B4_2309 0.51 -0.0 2,630,789 - -2.6 2,630,789 - Pf1N1B4_2309 0.51 -0.0 2,630,839 + Pf1N1B4_2309 0.57 -0.0 2,630,885 + Pf1N1B4_2309 0.63 -0.0 2,630,885 + Pf1N1B4_2309 0.63 -0.0 2,630,885 + Pf1N1B4_2309 0.63 -0.0 2,630,885 + Pf1N1B4_2309 0.63 -4.3 2,630,885 + Pf1N1B4_2309 0.63 -0.0 2,630,890 - Pf1N1B4_2309 0.63 -0.0 2,630,961 + Pf1N1B4_2309 0.72 -0.0 2,630,962 - Pf1N1B4_2309 0.72 -0.0 2,631,063 + Pf1N1B4_2309 0.85 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -3.1 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -3.5 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -2.2 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,079 + Pf1N1B4_2309 0.86 -0.0 2,631,080 - Pf1N1B4_2309 0.87 -0.0 2,631,080 - Pf1N1B4_2309 0.87 -0.9 2,631,080 - Pf1N1B4_2309 0.87 -0.0 2,631,080 - Pf1N1B4_2309 0.87 -0.0 2,631,080 - Pf1N1B4_2309 0.87 -2.0 2,631,080 - Pf1N1B4_2309 0.87 -2.1 2,631,080 - Pf1N1B4_2309 0.87 -1.4 2,631,087 + -0.0 2,631,087 + Pf1N1B4_2309 0.87 -0.0 2,631,196 + -0.0 2,631,203 + -2.6 2,631,203 + -0.0 2,631,203 + -0.0 2,631,203 + -0.0 2,631,203 + -0.0 2,631,203 + -2.6 2,631,203 + -1.3 2,631,204 - -0.0 2,631,204 - -1.5 2,631,204 - -0.0 2,631,204 - -2.1 2,631,204 - -4.9 2,631,204 - -3.4 2,631,205 + -0.0 2,631,205 + -0.0 2,631,571 + -0.0 2,631,571 + -0.0 2,631,571 + -0.0 2,631,571 + -0.0 2,631,571 + -0.0 2,631,571 + -0.0 2,631,599 + -0.0 2,631,600 - -0.0 2,631,600 - -2.4 2,631,689 + -0.0 2,631,707 - Pf1N1B4_2311 0.10 -0.0 2,631,707 - Pf1N1B4_2311 0.10 -0.0 2,631,936 - -0.0 2,631,969 - -2.9 2,632,056 - -1.7 2,632,082 + -0.0 2,632,082 + -0.0 2,632,089 + -0.0
Or see this region's nucleotide sequence