Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1818 and Pf1N1B4_1819 are separated by 15 nucleotides Pf1N1B4_1819 and Pf1N1B4_1820 overlap by 1 nucleotides
Pf1N1B4_1818 - NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2), at 2,096,087 to 2,097,208
_1818
Pf1N1B4_1819 - NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2), at 2,097,224 to 2,097,547
_1819
Pf1N1B4_1820 - NAD(P) transhydrogenase subunit beta (EC 1.6.1.2), at 2,097,547 to 2,098,983
_1820
Position (kb)
2097
2098 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2096.234 kb on + strand, within Pf1N1B4_1818 at 2096.234 kb on + strand, within Pf1N1B4_1818 at 2096.234 kb on + strand, within Pf1N1B4_1818 at 2096.234 kb on + strand, within Pf1N1B4_1818 at 2096.235 kb on - strand, within Pf1N1B4_1818 at 2096.335 kb on - strand, within Pf1N1B4_1818 at 2096.499 kb on - strand at 2096.499 kb on - strand at 2096.501 kb on - strand, within Pf1N1B4_1818 at 2096.525 kb on + strand, within Pf1N1B4_1818 at 2096.525 kb on + strand, within Pf1N1B4_1818 at 2096.525 kb on + strand, within Pf1N1B4_1818 at 2096.526 kb on - strand, within Pf1N1B4_1818 at 2096.528 kb on + strand, within Pf1N1B4_1818 at 2096.730 kb on - strand, within Pf1N1B4_1818 at 2096.857 kb on + strand, within Pf1N1B4_1818 at 2096.857 kb on + strand, within Pf1N1B4_1818 at 2096.858 kb on - strand, within Pf1N1B4_1818 at 2096.860 kb on + strand, within Pf1N1B4_1818 at 2096.860 kb on + strand, within Pf1N1B4_1818 at 2096.860 kb on + strand, within Pf1N1B4_1818 at 2096.860 kb on + strand, within Pf1N1B4_1818 at 2096.861 kb on - strand, within Pf1N1B4_1818 at 2096.861 kb on - strand, within Pf1N1B4_1818 at 2096.861 kb on - strand, within Pf1N1B4_1818 at 2096.896 kb on - strand, within Pf1N1B4_1818 at 2097.074 kb on + strand, within Pf1N1B4_1818 at 2097.206 kb on + strand at 2097.207 kb on - strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.235 kb on + strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.236 kb on - strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.251 kb on + strand at 2097.252 kb on - strand at 2097.252 kb on - strand at 2097.252 kb on - strand at 2097.252 kb on - strand at 2097.252 kb on - strand at 2097.252 kb on - strand at 2097.281 kb on + strand, within Pf1N1B4_1819 at 2097.281 kb on + strand, within Pf1N1B4_1819 at 2097.281 kb on + strand, within Pf1N1B4_1819 at 2097.281 kb on + strand, within Pf1N1B4_1819 at 2097.282 kb on - strand, within Pf1N1B4_1819 at 2097.282 kb on - strand, within Pf1N1B4_1819 at 2097.310 kb on + strand, within Pf1N1B4_1819 at 2097.310 kb on + strand, within Pf1N1B4_1819 at 2097.311 kb on - strand, within Pf1N1B4_1819 at 2097.311 kb on - strand, within Pf1N1B4_1819 at 2097.574 kb on + strand at 2097.575 kb on - strand at 2097.653 kb on - strand at 2097.688 kb on - strand at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.703 kb on + strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.704 kb on - strand, within Pf1N1B4_1820 at 2097.705 kb on + strand, within Pf1N1B4_1820 at 2097.705 kb on + strand, within Pf1N1B4_1820 at 2097.705 kb on + strand, within Pf1N1B4_1820 at 2097.705 kb on + strand, within Pf1N1B4_1820 at 2097.706 kb on - strand, within Pf1N1B4_1820 at 2097.706 kb on - strand, within Pf1N1B4_1820 at 2097.706 kb on - strand, within Pf1N1B4_1820 at 2097.727 kb on - strand, within Pf1N1B4_1820 at 2097.743 kb on - strand, within Pf1N1B4_1820 at 2097.743 kb on - strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.752 kb on + strand, within Pf1N1B4_1820 at 2097.753 kb on - strand, within Pf1N1B4_1820 at 2097.753 kb on - strand, within Pf1N1B4_1820 at 2097.853 kb on - strand, within Pf1N1B4_1820 at 2098.030 kb on + strand, within Pf1N1B4_1820 at 2098.030 kb on + strand, within Pf1N1B4_1820 at 2098.030 kb on + strand, within Pf1N1B4_1820 at 2098.030 kb on + strand, within Pf1N1B4_1820 at 2098.031 kb on - strand, within Pf1N1B4_1820 at 2098.031 kb on - strand, within Pf1N1B4_1820 at 2098.031 kb on - strand, within Pf1N1B4_1820 at 2098.031 kb on - strand, within Pf1N1B4_1820 at 2098.031 kb on - strand at 2098.061 kb on + strand, within Pf1N1B4_1820 at 2098.062 kb on - strand, within Pf1N1B4_1820 at 2098.148 kb on + strand, within Pf1N1B4_1820 at 2098.148 kb on + strand, within Pf1N1B4_1820 at 2098.148 kb on + strand, within Pf1N1B4_1820 at 2098.149 kb on - strand, within Pf1N1B4_1820 at 2098.149 kb on - strand, within Pf1N1B4_1820 at 2098.255 kb on + strand, within Pf1N1B4_1820 at 2098.255 kb on + strand, within Pf1N1B4_1820 at 2098.255 kb on + strand, within Pf1N1B4_1820 at 2098.255 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.354 kb on + strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.355 kb on - strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.359 kb on + strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.360 kb on - strand, within Pf1N1B4_1820 at 2098.488 kb on + strand, within Pf1N1B4_1820
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 2,096,234 + Pf1N1B4_1818 0.13 -3.2 2,096,234 + Pf1N1B4_1818 0.13 -3.3 2,096,234 + Pf1N1B4_1818 0.13 -2.3 2,096,234 + Pf1N1B4_1818 0.13 -0.0 2,096,235 - Pf1N1B4_1818 0.13 -0.0 2,096,335 - Pf1N1B4_1818 0.22 -0.0 2,096,499 - -0.0 2,096,499 - -0.0 2,096,501 - Pf1N1B4_1818 0.37 -3.9 2,096,525 + Pf1N1B4_1818 0.39 -3.2 2,096,525 + Pf1N1B4_1818 0.39 -0.0 2,096,525 + Pf1N1B4_1818 0.39 -4.7 2,096,526 - Pf1N1B4_1818 0.39 -0.0 2,096,528 + Pf1N1B4_1818 0.39 -0.0 2,096,730 - Pf1N1B4_1818 0.57 -4.8 2,096,857 + Pf1N1B4_1818 0.69 -4.5 2,096,857 + Pf1N1B4_1818 0.69 -3.7 2,096,858 - Pf1N1B4_1818 0.69 -1.6 2,096,860 + Pf1N1B4_1818 0.69 -5.0 2,096,860 + Pf1N1B4_1818 0.69 -3.3 2,096,860 + Pf1N1B4_1818 0.69 -0.0 2,096,860 + Pf1N1B4_1818 0.69 -0.0 2,096,861 - Pf1N1B4_1818 0.69 -0.0 2,096,861 - Pf1N1B4_1818 0.69 -5.8 2,096,861 - Pf1N1B4_1818 0.69 -3.6 2,096,896 - Pf1N1B4_1818 0.72 -0.0 2,097,074 + Pf1N1B4_1818 0.88 -3.6 2,097,206 + -1.9 2,097,207 - -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -0.0 2,097,235 + -3.5 2,097,235 + -0.0 2,097,235 + -3.6 2,097,235 + -2.9 2,097,236 - -0.0 2,097,236 - -0.0 2,097,236 - -0.0 2,097,236 - -2.9 2,097,236 - -3.6 2,097,236 - -0.0 2,097,236 - -2.6 2,097,236 - -0.0 2,097,236 - -0.0 2,097,236 - -0.0 2,097,251 + -0.0 2,097,251 + -2.5 2,097,251 + -0.0 2,097,251 + -0.0 2,097,251 + -0.0 2,097,251 + -3.6 2,097,251 + -0.0 2,097,251 + -3.9 2,097,251 + -3.3 2,097,252 - -0.0 2,097,252 - -0.0 2,097,252 - -0.0 2,097,252 - -3.7 2,097,252 - -0.0 2,097,252 - -3.6 2,097,281 + Pf1N1B4_1819 0.18 -3.7 2,097,281 + Pf1N1B4_1819 0.18 -0.0 2,097,281 + Pf1N1B4_1819 0.18 -0.0 2,097,281 + Pf1N1B4_1819 0.18 -0.0 2,097,282 - Pf1N1B4_1819 0.18 -0.0 2,097,282 - Pf1N1B4_1819 0.18 -0.0 2,097,310 + Pf1N1B4_1819 0.27 -5.5 2,097,310 + Pf1N1B4_1819 0.27 -0.0 2,097,311 - Pf1N1B4_1819 0.27 -3.2 2,097,311 - Pf1N1B4_1819 0.27 -0.0 2,097,574 + -3.9 2,097,575 - -0.0 2,097,653 - -0.0 2,097,688 - -5.8 2,097,703 + Pf1N1B4_1820 0.11 -5.9 2,097,703 + Pf1N1B4_1820 0.11 -4.5 2,097,703 + Pf1N1B4_1820 0.11 -0.0 2,097,703 + Pf1N1B4_1820 0.11 -3.7 2,097,703 + Pf1N1B4_1820 0.11 -0.0 2,097,703 + Pf1N1B4_1820 0.11 -3.3 2,097,703 + Pf1N1B4_1820 0.11 -0.0 2,097,703 + Pf1N1B4_1820 0.11 -0.0 2,097,703 + Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -1.8 2,097,704 - Pf1N1B4_1820 0.11 -4.9 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -4.5 2,097,704 - Pf1N1B4_1820 0.11 -5.7 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -4.9 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,704 - Pf1N1B4_1820 0.11 -0.0 2,097,705 + Pf1N1B4_1820 0.11 -3.6 2,097,705 + Pf1N1B4_1820 0.11 -3.9 2,097,705 + Pf1N1B4_1820 0.11 -4.5 2,097,705 + Pf1N1B4_1820 0.11 -5.4 2,097,706 - Pf1N1B4_1820 0.11 -0.0 2,097,706 - Pf1N1B4_1820 0.11 -0.0 2,097,706 - Pf1N1B4_1820 0.11 -3.3 2,097,727 - Pf1N1B4_1820 0.13 -4.6 2,097,743 - Pf1N1B4_1820 0.14 -0.0 2,097,743 - Pf1N1B4_1820 0.14 -0.0 2,097,752 + Pf1N1B4_1820 0.14 -0.0 2,097,752 + Pf1N1B4_1820 0.14 -0.0 2,097,752 + Pf1N1B4_1820 0.14 -0.0 2,097,752 + Pf1N1B4_1820 0.14 -2.5 2,097,752 + -0.0 2,097,752 + Pf1N1B4_1820 0.14 -2.7 2,097,752 + Pf1N1B4_1820 0.14 -0.0 2,097,752 + Pf1N1B4_1820 0.14 -4.2 2,097,752 + Pf1N1B4_1820 0.14 -2.2 2,097,752 + Pf1N1B4_1820 0.14 -5.5 2,097,753 - Pf1N1B4_1820 0.14 -3.0 2,097,753 - Pf1N1B4_1820 0.14 -3.2 2,097,853 - Pf1N1B4_1820 0.21 -0.0 2,098,030 + Pf1N1B4_1820 0.34 -0.0 2,098,030 + Pf1N1B4_1820 0.34 -0.0 2,098,030 + Pf1N1B4_1820 0.34 -4.6 2,098,030 + Pf1N1B4_1820 0.34 -0.0 2,098,031 - Pf1N1B4_1820 0.34 -5.4 2,098,031 - Pf1N1B4_1820 0.34 -0.0 2,098,031 - Pf1N1B4_1820 0.34 -3.8 2,098,031 - Pf1N1B4_1820 0.34 -0.0 2,098,031 - -4.9 2,098,061 + Pf1N1B4_1820 0.36 -4.3 2,098,062 - Pf1N1B4_1820 0.36 -0.0 2,098,148 + Pf1N1B4_1820 0.42 -0.0 2,098,148 + Pf1N1B4_1820 0.42 -0.0 2,098,148 + Pf1N1B4_1820 0.42 -0.0 2,098,149 - Pf1N1B4_1820 0.42 -0.0 2,098,149 - Pf1N1B4_1820 0.42 -0.0 2,098,255 + Pf1N1B4_1820 0.49 -3.1 2,098,255 + Pf1N1B4_1820 0.49 -0.0 2,098,255 + Pf1N1B4_1820 0.49 -0.0 2,098,255 + Pf1N1B4_1820 0.49 -3.6 2,098,354 + Pf1N1B4_1820 0.56 -0.0 2,098,354 + Pf1N1B4_1820 0.56 -0.0 2,098,354 + Pf1N1B4_1820 0.56 -0.0 2,098,354 + Pf1N1B4_1820 0.56 -3.0 2,098,354 + Pf1N1B4_1820 0.56 -5.1 2,098,354 + Pf1N1B4_1820 0.56 -0.0 2,098,354 + Pf1N1B4_1820 0.56 -0.0 2,098,354 + Pf1N1B4_1820 0.56 -2.2 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,355 - Pf1N1B4_1820 0.56 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -4.1 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -3.7 2,098,359 + Pf1N1B4_1820 0.57 -1.2 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,359 + Pf1N1B4_1820 0.57 -0.0 2,098,360 - Pf1N1B4_1820 0.57 -3.1 2,098,360 - Pf1N1B4_1820 0.57 -0.0 2,098,360 - Pf1N1B4_1820 0.57 -3.4 2,098,360 - Pf1N1B4_1820 0.57 -0.0 2,098,360 - Pf1N1B4_1820 0.57 -0.0 2,098,360 - Pf1N1B4_1820 0.57 -0.0 2,098,360 - Pf1N1B4_1820 0.57 -0.0 2,098,488 + Pf1N1B4_1820 0.65 -0.0
Or see this region's nucleotide sequence