Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_132 and Pf1N1B4_133 are separated by 144 nucleotides Pf1N1B4_133 and Pf1N1B4_134 are separated by 301 nucleotides
Pf1N1B4_132 - L-ornithine 5-monooxygenase (EC 1.13.12.-), PvdA of pyoverdin biosynthesis, at 145,701 to 147,038
_132
Pf1N1B4_133 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis, at 147,183 to 147,665
_133
Pf1N1B4_134 - pyoverdine-specific efflux macA-like protein, at 147,967 to 149,127
_134
Position (kb)
147
148 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 146.185 kb on + strand, within Pf1N1B4_132 at 146.186 kb on - strand, within Pf1N1B4_132 at 146.186 kb on - strand, within Pf1N1B4_132 at 146.713 kb on + strand, within Pf1N1B4_132 at 146.713 kb on + strand, within Pf1N1B4_132 at 146.714 kb on - strand, within Pf1N1B4_132 at 146.731 kb on + strand, within Pf1N1B4_132 at 146.732 kb on - strand, within Pf1N1B4_132 at 146.782 kb on + strand, within Pf1N1B4_132 at 147.421 kb on + strand, within Pf1N1B4_133 at 147.422 kb on - strand at 147.442 kb on - strand at 147.442 kb on - strand, within Pf1N1B4_133 at 147.442 kb on - strand, within Pf1N1B4_133 at 147.442 kb on - strand, within Pf1N1B4_133 at 147.586 kb on - strand, within Pf1N1B4_133 at 147.616 kb on - strand, within Pf1N1B4_133 at 147.635 kb on - strand at 147.835 kb on + strand at 148.147 kb on + strand, within Pf1N1B4_134 at 148.147 kb on + strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.242 kb on - strand, within Pf1N1B4_134 at 148.352 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 146,185 + Pf1N1B4_132 0.36 -1.9 146,186 - Pf1N1B4_132 0.36 -0.0 146,186 - Pf1N1B4_132 0.36 -3.1 146,713 + Pf1N1B4_132 0.76 -0.0 146,713 + Pf1N1B4_132 0.76 -0.0 146,714 - Pf1N1B4_132 0.76 -0.0 146,731 + Pf1N1B4_132 0.77 -2.8 146,732 - Pf1N1B4_132 0.77 -2.4 146,782 + Pf1N1B4_132 0.81 -0.0 147,421 + Pf1N1B4_133 0.49 -0.0 147,422 - -2.8 147,442 - -0.0 147,442 - Pf1N1B4_133 0.54 -3.0 147,442 - Pf1N1B4_133 0.54 -2.4 147,442 - Pf1N1B4_133 0.54 -3.1 147,586 - Pf1N1B4_133 0.83 -0.0 147,616 - Pf1N1B4_133 0.90 -3.4 147,635 - -0.0 147,835 + -0.0 148,147 + Pf1N1B4_134 0.16 -2.0 148,147 + Pf1N1B4_134 0.16 -3.4 148,148 - Pf1N1B4_134 0.16 -1.6 148,148 - Pf1N1B4_134 0.16 -0.0 148,148 - Pf1N1B4_134 0.16 -0.0 148,148 - Pf1N1B4_134 0.16 -1.8 148,241 + Pf1N1B4_134 0.24 -0.0 148,241 + Pf1N1B4_134 0.24 -1.3 148,241 + Pf1N1B4_134 0.24 -0.0 148,241 + Pf1N1B4_134 0.24 -0.0 148,242 - Pf1N1B4_134 0.24 -0.0 148,352 - Pf1N1B4_134 0.33 -0.0 148,526 - Pf1N1B4_134 0.48 -0.0 148,526 - Pf1N1B4_134 0.48 -0.0 148,526 - Pf1N1B4_134 0.48 -2.6
Or see this region's nucleotide sequence