Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1262

Experiment: Nitrate reduction, replicate C t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1260 and Pf1N1B4_1261 are separated by 148 nucleotidesPf1N1B4_1261 and Pf1N1B4_1262 are separated by 191 nucleotidesPf1N1B4_1262 and Pf1N1B4_1263 overlap by 4 nucleotidesPf1N1B4_1263 and Pf1N1B4_1264 overlap by 47 nucleotides Pf1N1B4_1260 - Copper chaperone, at 1,440,981 to 1,441,178 _1260 Pf1N1B4_1261 - FIG00953575: hypothetical protein, at 1,441,327 to 1,441,710 _1261 Pf1N1B4_1262 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 1,441,902 to 1,444,295 _1262 Pf1N1B4_1263 - Cu(I)-responsive transcriptional regulator, at 1,444,292 to 1,444,705 _1263 Pf1N1B4_1264 - Integral membrane efflux protein, at 1,444,659 to 1,445,873 _1264 Position (kb) 1441 1442 1443 1444 1445Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1440.970 kb on + strandat 1440.974 kb on - strandat 1441.000 kb on + strandat 1441.000 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.300 kb on + strandat 1441.301 kb on - strandat 1441.301 kb on - strandat 1441.301 kb on - strandat 1441.301 kb on - strandat 1441.301 kb on - strandat 1441.301 kb on - strandat 1441.367 kb on + strand, within Pf1N1B4_1261at 1441.367 kb on + strand, within Pf1N1B4_1261at 1441.367 kb on + strand, within Pf1N1B4_1261at 1441.367 kb on + strand, within Pf1N1B4_1261at 1441.367 kb on + strand, within Pf1N1B4_1261at 1441.368 kb on - strand, within Pf1N1B4_1261at 1441.408 kb on + strandat 1441.471 kb on + strand, within Pf1N1B4_1261at 1441.472 kb on - strand, within Pf1N1B4_1261at 1441.472 kb on - strand, within Pf1N1B4_1261at 1441.472 kb on - strand, within Pf1N1B4_1261at 1441.488 kb on - strand, within Pf1N1B4_1261at 1441.681 kb on + strandat 1441.681 kb on + strandat 1441.681 kb on + strandat 1441.681 kb on + strandat 1441.681 kb on + strandat 1441.681 kb on + strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.682 kb on - strandat 1441.875 kb on - strandat 1441.875 kb on - strandat 1441.875 kb on - strandat 1441.878 kb on + strandat 1441.878 kb on + strandat 1441.886 kb on - strandat 1442.299 kb on - strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.514 kb on + strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strandat 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.515 kb on - strand, within Pf1N1B4_1262at 1442.646 kb on + strand, within Pf1N1B4_1262at 1442.646 kb on + strand, within Pf1N1B4_1262at 1442.646 kb on + strand, within Pf1N1B4_1262at 1442.646 kb on + strand, within Pf1N1B4_1262at 1442.646 kb on + strand, within Pf1N1B4_1262at 1442.665 kb on + strand, within Pf1N1B4_1262at 1442.694 kb on + strand, within Pf1N1B4_1262at 1442.694 kb on + strand, within Pf1N1B4_1262at 1442.694 kb on + strand, within Pf1N1B4_1262at 1442.695 kb on - strand, within Pf1N1B4_1262at 1442.695 kb on - strand, within Pf1N1B4_1262at 1442.695 kb on - strand, within Pf1N1B4_1262at 1443.165 kb on + strand, within Pf1N1B4_1262at 1443.785 kb on - strand, within Pf1N1B4_1262at 1444.108 kb on - strandat 1444.146 kb on + strandat 1444.146 kb on + strandat 1444.146 kb on + strandat 1444.147 kb on - strandat 1444.147 kb on - strandat 1444.147 kb on - strandat 1444.249 kb on + strandat 1444.349 kb on + strand, within Pf1N1B4_1263at 1444.349 kb on + strand, within Pf1N1B4_1263at 1444.406 kb on + strand, within Pf1N1B4_1263at 1444.426 kb on + strand, within Pf1N1B4_1263at 1444.427 kb on - strand, within Pf1N1B4_1263at 1444.618 kb on - strand, within Pf1N1B4_1263at 1444.789 kb on + strand, within Pf1N1B4_1264at 1444.790 kb on - strand, within Pf1N1B4_1264at 1444.964 kb on - strand, within Pf1N1B4_1264at 1445.055 kb on - strand, within Pf1N1B4_1264

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1
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1,440,970 + +0.0
1,440,974 - +0.0
1,441,000 + +0.0
1,441,000 + +0.0
1,441,300 + -2.0
1,441,300 + +0.0
1,441,300 + -1.1
1,441,300 + +0.0
1,441,300 + -2.1
1,441,300 + -2.8
1,441,300 + +0.0
1,441,300 + +0.0
1,441,300 + +0.0
1,441,301 - +0.0
1,441,301 - -2.4
1,441,301 - +0.0
1,441,301 - +0.0
1,441,301 - -1.4
1,441,301 - +0.0
1,441,367 + Pf1N1B4_1261 0.10 -2.8
1,441,367 + Pf1N1B4_1261 0.10 +0.0
1,441,367 + Pf1N1B4_1261 0.10 +0.0
1,441,367 + Pf1N1B4_1261 0.10 +0.0
1,441,367 + Pf1N1B4_1261 0.10 +0.0
1,441,368 - Pf1N1B4_1261 0.11 -3.6
1,441,408 + -1.6
1,441,471 + Pf1N1B4_1261 0.38 +0.0
1,441,472 - Pf1N1B4_1261 0.38 +0.0
1,441,472 - Pf1N1B4_1261 0.38 +0.0
1,441,472 - Pf1N1B4_1261 0.38 +0.0
1,441,488 - Pf1N1B4_1261 0.42 +0.0
1,441,681 + +0.0
1,441,681 + +0.0
1,441,681 + +0.0
1,441,681 + +0.0
1,441,681 + -2.3
1,441,681 + +0.0
1,441,682 - +0.0
1,441,682 - -2.3
1,441,682 - +0.0
1,441,682 - -2.7
1,441,682 - +0.0
1,441,682 - +0.0
1,441,682 - -1.4
1,441,875 - +0.0
1,441,875 - +0.0
1,441,875 - +0.0
1,441,878 + -1.2
1,441,878 + +0.0
1,441,886 - -2.2
1,442,299 - Pf1N1B4_1262 0.17 +0.0
1,442,514 + Pf1N1B4_1262 0.26 -3.2
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 +0.0
1,442,514 + Pf1N1B4_1262 0.26 -4.1
1,442,514 + Pf1N1B4_1262 0.26 -3.8
1,442,515 - Pf1N1B4_1262 0.26 -5.3
1,442,515 - Pf1N1B4_1262 0.26 -5.0
1,442,515 - +0.0
1,442,515 - Pf1N1B4_1262 0.26 +0.0
1,442,515 - Pf1N1B4_1262 0.26 +0.0
1,442,515 - Pf1N1B4_1262 0.26 +0.0
1,442,515 - Pf1N1B4_1262 0.26 -2.8
1,442,515 - Pf1N1B4_1262 0.26 +0.0
1,442,646 + Pf1N1B4_1262 0.31 +0.0
1,442,646 + Pf1N1B4_1262 0.31 +0.0
1,442,646 + Pf1N1B4_1262 0.31 +0.0
1,442,646 + Pf1N1B4_1262 0.31 +0.0
1,442,646 + Pf1N1B4_1262 0.31 +0.0
1,442,665 + Pf1N1B4_1262 0.32 -1.3
1,442,694 + Pf1N1B4_1262 0.33 +0.0
1,442,694 + Pf1N1B4_1262 0.33 +0.0
1,442,694 + Pf1N1B4_1262 0.33 +0.0
1,442,695 - Pf1N1B4_1262 0.33 -3.7
1,442,695 - Pf1N1B4_1262 0.33 +0.0
1,442,695 - Pf1N1B4_1262 0.33 -1.0
1,443,165 + Pf1N1B4_1262 0.53 +0.0
1,443,785 - Pf1N1B4_1262 0.79 -3.8
1,444,108 - -4.4
1,444,146 + +0.0
1,444,146 + -2.5
1,444,146 + -3.5
1,444,147 - +0.0
1,444,147 - +0.0
1,444,147 - +1.0
1,444,249 + -2.3
1,444,349 + Pf1N1B4_1263 0.14 -2.0
1,444,349 + Pf1N1B4_1263 0.14 -3.1
1,444,406 + Pf1N1B4_1263 0.28 -1.8
1,444,426 + Pf1N1B4_1263 0.32 +0.0
1,444,427 - Pf1N1B4_1263 0.33 -1.7
1,444,618 - Pf1N1B4_1263 0.79 +0.0
1,444,789 + Pf1N1B4_1264 0.11 -1.3
1,444,790 - Pf1N1B4_1264 0.11 -2.7
1,444,964 - Pf1N1B4_1264 0.25 +0.0
1,445,055 - Pf1N1B4_1264 0.33 +0.0

Or see this region's nucleotide sequence