Experiment: Nitrate reduction, replicate C t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1245 and Pf1N1B4_1246 are separated by 80 nucleotides Pf1N1B4_1246 and Pf1N1B4_1247 are separated by 82 nucleotides Pf1N1B4_1247 and Pf1N1B4_1248 are separated by 25 nucleotides Pf1N1B4_1248 and Pf1N1B4_1249 are separated by 254 nucleotides
Pf1N1B4_1245 - Nucleoside ABC transporter, permease protein 2, at 1,424,043 to 1,424,969
_1245
Pf1N1B4_1246 - FIG00954047: hypothetical protein, at 1,425,050 to 1,425,472
_1246
Pf1N1B4_1247 - transcriptional regulator, MerR family, at 1,425,555 to 1,425,905
_1247
Pf1N1B4_1248 - Probable short-chain dehydrogenase, at 1,425,931 to 1,426,617
_1248
Pf1N1B4_1249 - Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3), at 1,426,872 to 1,428,230
_1249
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1424.968 kb on - strand at 1425.171 kb on + strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.213 kb on + strand, within Pf1N1B4_1246 at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.572 kb on - strand at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.696 kb on + strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.726 kb on - strand, within Pf1N1B4_1247 at 1425.896 kb on - strand at 1426.129 kb on - strand, within Pf1N1B4_1248 at 1426.378 kb on + strand at 1426.378 kb on + strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.558 kb on + strand at 1426.558 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1427.079 kb on + strand, within Pf1N1B4_1249 at 1427.080 kb on - strand, within Pf1N1B4_1249 at 1427.080 kb on - strand, within Pf1N1B4_1249 at 1427.253 kb on + strand, within Pf1N1B4_1249 at 1427.253 kb on + strand, within Pf1N1B4_1249 at 1427.254 kb on - strand, within Pf1N1B4_1249 at 1427.518 kb on + strand, within Pf1N1B4_1249 at 1427.519 kb on - strand, within Pf1N1B4_1249 at 1427.519 kb on - strand, within Pf1N1B4_1249 at 1427.546 kb on + strand, within Pf1N1B4_1249 at 1427.557 kb on + strand, within Pf1N1B4_1249 at 1427.558 kb on - strand, within Pf1N1B4_1249 at 1427.558 kb on - strand, within Pf1N1B4_1249
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t1 remove 1,424,968 - -1.4 1,425,171 + Pf1N1B4_1246 0.29 -0.0 1,425,172 - Pf1N1B4_1246 0.29 -2.6 1,425,172 - Pf1N1B4_1246 0.29 -3.0 1,425,172 - Pf1N1B4_1246 0.29 -0.0 1,425,213 + Pf1N1B4_1246 0.39 -0.0 1,425,520 + -0.0 1,425,520 + -0.0 1,425,520 + -0.0 1,425,520 + -1.5 1,425,520 + -0.1 1,425,520 + -1.4 1,425,521 - -1.7 1,425,521 - -0.0 1,425,521 - -3.7 1,425,521 - -0.0 1,425,521 - -0.0 1,425,572 - -0.0 1,425,644 + Pf1N1B4_1247 0.25 -0.0 1,425,644 + Pf1N1B4_1247 0.25 -2.7 1,425,696 + Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -0.4 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,697 - Pf1N1B4_1247 0.40 -2.2 1,425,697 - Pf1N1B4_1247 0.40 -0.0 1,425,726 - Pf1N1B4_1247 0.49 -0.0 1,425,896 - -1.7 1,426,129 - Pf1N1B4_1248 0.29 -0.0 1,426,378 + -1.6 1,426,378 + Pf1N1B4_1248 0.65 -0.0 1,426,379 - Pf1N1B4_1248 0.65 -3.4 1,426,379 - Pf1N1B4_1248 0.65 -0.0 1,426,379 - Pf1N1B4_1248 0.65 -0.0 1,426,379 - Pf1N1B4_1248 0.65 -2.2 1,426,488 + Pf1N1B4_1248 0.81 -3.2 1,426,488 + Pf1N1B4_1248 0.81 -0.0 1,426,488 + Pf1N1B4_1248 0.81 -3.2 1,426,558 + -3.5 1,426,558 + -0.0 1,426,591 + -2.6 1,426,591 + -0.0 1,426,591 + -0.0 1,426,591 + -0.0 1,426,591 + -0.0 1,426,592 - -0.0 1,426,592 - -0.0 1,426,592 - -0.0 1,426,592 - -0.0 1,427,079 + Pf1N1B4_1249 0.15 +0.0 1,427,080 - Pf1N1B4_1249 0.15 +0.0 1,427,080 - Pf1N1B4_1249 0.15 -2.3 1,427,253 + Pf1N1B4_1249 0.28 +0.0 1,427,253 + Pf1N1B4_1249 0.28 +0.0 1,427,254 - Pf1N1B4_1249 0.28 -2.1 1,427,518 + Pf1N1B4_1249 0.48 +0.0 1,427,519 - Pf1N1B4_1249 0.48 -2.2 1,427,519 - Pf1N1B4_1249 0.48 +0.0 1,427,546 + Pf1N1B4_1249 0.50 +0.0 1,427,557 + Pf1N1B4_1249 0.50 +0.0 1,427,558 - Pf1N1B4_1249 0.50 -1.9 1,427,558 - Pf1N1B4_1249 0.50 -2.0
Or see this region's nucleotide sequence