Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26135 and BWI76_RS26140 are separated by 62 nucleotides BWI76_RS26140 and BWI76_RS26145 overlap by 4 nucleotides BWI76_RS26145 and BWI76_RS26150 are separated by 24 nucleotides
BWI76_RS26135: BWI76_RS26135 - Intracellular growth attenuator protein igaA, at 5,399,028 to 5,401,157
_RS26135
BWI76_RS26140: BWI76_RS26140 - GMP/IMP nucleotidase, at 5,401,220 to 5,401,903
_RS26140
BWI76_RS26145: BWI76_RS26145 - heat-shock protein Hsp15, at 5,401,900 to 5,402,301
_RS26145
BWI76_RS26150: BWI76_RS26150 - molecular chaperone Hsp33, at 5,402,326 to 5,403,204
_RS26150
Position (kb) 5401
5402 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5401.280 kb on + strand at 5401.350 kb on + strand, within BWI76_RS26140 at 5401.432 kb on + strand, within BWI76_RS26140 at 5401.438 kb on - strand, within BWI76_RS26140 at 5401.440 kb on - strand, within BWI76_RS26140 at 5401.492 kb on - strand, within BWI76_RS26140 at 5401.636 kb on + strand, within BWI76_RS26140 at 5401.850 kb on - strand at 5401.886 kb on + strand at 5402.012 kb on + strand, within BWI76_RS26145 at 5402.020 kb on - strand, within BWI76_RS26145 at 5402.020 kb on - strand, within BWI76_RS26145 at 5402.111 kb on + strand, within BWI76_RS26145 at 5402.477 kb on + strand, within BWI76_RS26150 at 5402.696 kb on - strand, within BWI76_RS26150 at 5402.770 kb on - strand, within BWI76_RS26150 at 5402.838 kb on + strand, within BWI76_RS26150 at 5402.892 kb on - strand, within BWI76_RS26150
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 5,401,280 + +0.8 5,401,350 + BWI76_RS26140 0.19 -1.1 5,401,432 + BWI76_RS26140 0.31 -1.5 5,401,438 - BWI76_RS26140 0.32 -0.4 5,401,440 - BWI76_RS26140 0.32 -1.5 5,401,492 - BWI76_RS26140 0.40 -1.0 5,401,636 + BWI76_RS26140 0.61 +0.4 5,401,850 - +0.7 5,401,886 + +0.2 5,402,012 + BWI76_RS26145 0.28 -0.4 5,402,020 - BWI76_RS26145 0.30 -0.4 5,402,020 - BWI76_RS26145 0.30 -1.1 5,402,111 + BWI76_RS26145 0.52 -0.2 5,402,477 + BWI76_RS26150 0.17 +3.9 5,402,696 - BWI76_RS26150 0.42 +0.7 5,402,770 - BWI76_RS26150 0.51 +0.1 5,402,838 + BWI76_RS26150 0.58 +0.2 5,402,892 - BWI76_RS26150 0.64 -0.6
Or see this region's nucleotide sequence