Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25365

Experiment: Dilute LB (1/20)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25355 and BWI76_RS25360 are separated by 190 nucleotidesBWI76_RS25360 and BWI76_RS25365 are separated by 172 nucleotidesBWI76_RS25365 and BWI76_RS25370 are separated by 86 nucleotides BWI76_RS25355: BWI76_RS25355 - cell division protein ZapE, at 5,270,034 to 5,271,167 _RS25355 BWI76_RS25360: BWI76_RS25360 - hypothetical protein, at 5,271,358 to 5,271,756 _RS25360 BWI76_RS25365: BWI76_RS25365 - serine endoprotease DegQ, at 5,271,929 to 5,273,296 _RS25365 BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441 _RS25370 Position (kb) 5271 5272 5273 5274Strain fitness (log2 ratio) -2 -1 0 1at 5270.942 kb on - strand, within BWI76_RS25355at 5270.942 kb on - strand, within BWI76_RS25355at 5271.148 kb on + strandat 5271.200 kb on - strandat 5271.230 kb on + strandat 5271.238 kb on - strandat 5271.811 kb on + strandat 5271.853 kb on - strandat 5271.916 kb on - strandat 5271.962 kb on + strandat 5271.969 kb on + strandat 5272.107 kb on - strand, within BWI76_RS25365at 5272.203 kb on + strand, within BWI76_RS25365at 5272.211 kb on - strand, within BWI76_RS25365at 5272.463 kb on - strand, within BWI76_RS25365at 5272.463 kb on - strand, within BWI76_RS25365at 5272.947 kb on + strand, within BWI76_RS25365at 5272.948 kb on + strand, within BWI76_RS25365at 5273.146 kb on - strand, within BWI76_RS25365at 5273.152 kb on + strand, within BWI76_RS25365at 5273.341 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Dilute LB (1/20)
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5,270,942 - BWI76_RS25355 0.80 -0.2
5,270,942 - BWI76_RS25355 0.80 -0.1
5,271,148 + -0.7
5,271,200 - -0.4
5,271,230 + -0.5
5,271,238 - -0.1
5,271,811 + -0.1
5,271,853 - -1.5
5,271,916 - -0.4
5,271,962 + -0.5
5,271,969 + -0.3
5,272,107 - BWI76_RS25365 0.13 -0.4
5,272,203 + BWI76_RS25365 0.20 -0.8
5,272,211 - BWI76_RS25365 0.21 -0.4
5,272,463 - BWI76_RS25365 0.39 -1.5
5,272,463 - BWI76_RS25365 0.39 -0.3
5,272,947 + BWI76_RS25365 0.74 -0.4
5,272,948 + BWI76_RS25365 0.74 -0.5
5,273,146 - BWI76_RS25365 0.89 -2.6
5,273,152 + BWI76_RS25365 0.89 -0.9
5,273,341 + -0.1

Or see this region's nucleotide sequence