Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotides BWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotides BWI76_RS25230 and BWI76_RS25235 are separated by 209 nucleotides
BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605
_RS25220
BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392
_RS25225
BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212
_RS25230
BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399
_RS25235
Position (kb) 5246
5247
5248 Strain fitness (log2 ratio) -2
-1
0
1 at 5245.572 kb on + strand at 5246.024 kb on + strand, within BWI76_RS25225 at 5246.161 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.252 kb on - strand, within BWI76_RS25225 at 5246.294 kb on + strand, within BWI76_RS25225 at 5246.338 kb on - strand at 5246.538 kb on - strand, within BWI76_RS25230 at 5246.629 kb on + strand, within BWI76_RS25230 at 5246.740 kb on + strand, within BWI76_RS25230 at 5246.845 kb on - strand, within BWI76_RS25230 at 5246.909 kb on + strand, within BWI76_RS25230 at 5246.947 kb on - strand, within BWI76_RS25230 at 5246.968 kb on + strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5247.247 kb on - strand at 5247.379 kb on - strand at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 5,245,572 + +0.5 5,246,024 + BWI76_RS25225 0.53 -0.4 5,246,161 + BWI76_RS25225 0.70 -0.2 5,246,241 + BWI76_RS25225 0.81 +0.2 5,246,241 + BWI76_RS25225 0.81 -0.5 5,246,252 - BWI76_RS25225 0.82 -0.1 5,246,294 + BWI76_RS25225 0.87 +0.0 5,246,338 - -0.1 5,246,538 - BWI76_RS25230 0.17 -2.7 5,246,629 + BWI76_RS25230 0.28 -0.3 5,246,740 + BWI76_RS25230 0.42 -1.3 5,246,845 - BWI76_RS25230 0.55 -0.1 5,246,909 + BWI76_RS25230 0.63 -0.8 5,246,947 - BWI76_RS25230 0.67 -0.3 5,246,968 + BWI76_RS25230 0.70 -0.2 5,246,976 - BWI76_RS25230 0.71 -0.4 5,246,976 - BWI76_RS25230 0.71 -1.2 5,246,976 - BWI76_RS25230 0.71 -0.9 5,247,247 - -0.4 5,247,379 - -0.5 5,247,432 - +0.2 5,247,487 + -0.4 5,247,487 + +0.8 5,247,487 + +0.2 5,247,487 + -0.1 5,247,553 + BWI76_RS25235 0.13 +0.3 5,247,605 - BWI76_RS25235 0.19 +0.2 5,247,633 + BWI76_RS25235 0.22 -2.5 5,247,699 - BWI76_RS25235 0.28 +0.3 5,247,883 - BWI76_RS25235 0.47 -0.2 5,247,883 - +1.1 5,247,998 + BWI76_RS25235 0.59 +0.4 5,248,166 + BWI76_RS25235 0.76 +0.4
Or see this region's nucleotide sequence