Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotides BWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotides BWI76_RS19150 and BWI76_RS19155 are separated by 12 nucleotides
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633
_RS19145
BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532
_RS19150
BWI76_RS19155: BWI76_RS19155 - formate dehydrogenase-N subunit alpha, at 3,982,545 to 3,985,595
_RS19155
Position (kb)
3981
3982 Strain fitness (log2 ratio)
-1
0
1 at 3980.306 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.327 kb on - strand, within BWI76_RS19140 at 3980.484 kb on - strand, within BWI76_RS19140 at 3980.662 kb on + strand at 3981.070 kb on - strand, within BWI76_RS19145
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 3,980,306 + BWI76_RS19140 0.68 +0.1 3,980,319 + BWI76_RS19140 0.69 +0.2 3,980,319 + BWI76_RS19140 0.69 -0.7 3,980,327 - BWI76_RS19140 0.69 +0.2 3,980,484 - BWI76_RS19140 0.81 +0.1 3,980,662 + +1.2 3,981,070 - BWI76_RS19145 0.10 -0.9
Or see this region's nucleotide sequence