Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17845 and BWI76_RS17850 are separated by 525 nucleotides BWI76_RS17850 and BWI76_RS17855 overlap by 1 nucleotides
BWI76_RS17845: BWI76_RS17845 - extracellular solute-binding protein, at 3,709,020 to 3,710,309
_RS17845
BWI76_RS17850: BWI76_RS17850 - alginate lyase, at 3,710,835 to 3,712,073
_RS17850
BWI76_RS17855: BWI76_RS17855 - alginate lyase, at 3,712,073 to 3,714,103
_RS17855
Position (kb)
3710
3711
3712
3713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3709.953 kb on + strand, within BWI76_RS17845 at 3709.961 kb on - strand, within BWI76_RS17845 at 3710.027 kb on - strand, within BWI76_RS17845 at 3710.108 kb on + strand, within BWI76_RS17845 at 3710.116 kb on - strand, within BWI76_RS17845 at 3710.214 kb on + strand at 3710.214 kb on + strand at 3710.214 kb on + strand at 3710.223 kb on + strand at 3710.230 kb on - strand at 3710.231 kb on - strand at 3710.239 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.249 kb on + strand at 3710.252 kb on - strand at 3710.252 kb on - strand at 3710.252 kb on - strand at 3710.255 kb on + strand at 3710.255 kb on + strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.257 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.263 kb on - strand at 3710.307 kb on + strand at 3710.307 kb on + strand at 3710.313 kb on - strand at 3710.385 kb on + strand at 3710.701 kb on + strand at 3710.701 kb on + strand at 3710.709 kb on - strand at 3710.818 kb on + strand at 3710.833 kb on - strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.839 kb on + strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.847 kb on - strand at 3710.848 kb on - strand at 3710.857 kb on - strand at 3710.859 kb on + strand at 3710.861 kb on + strand at 3710.867 kb on - strand at 3710.869 kb on - strand at 3710.871 kb on + strand at 3710.876 kb on + strand at 3710.876 kb on + strand at 3710.879 kb on - strand at 3710.911 kb on - strand at 3710.921 kb on - strand at 3710.923 kb on + strand at 3710.923 kb on - strand at 3711.132 kb on + strand, within BWI76_RS17850 at 3711.133 kb on - strand, within BWI76_RS17850 at 3711.135 kb on - strand, within BWI76_RS17850 at 3711.142 kb on + strand, within BWI76_RS17850 at 3711.308 kb on + strand, within BWI76_RS17850 at 3711.310 kb on - strand, within BWI76_RS17850 at 3711.427 kb on - strand, within BWI76_RS17850 at 3711.460 kb on - strand, within BWI76_RS17850 at 3711.487 kb on + strand at 3711.541 kb on + strand, within BWI76_RS17850 at 3711.756 kb on + strand, within BWI76_RS17850 at 3711.831 kb on - strand, within BWI76_RS17850 at 3711.848 kb on - strand, within BWI76_RS17850 at 3712.068 kb on - strand at 3712.250 kb on - strand at 3712.456 kb on + strand, within BWI76_RS17855 at 3712.484 kb on + strand, within BWI76_RS17855 at 3712.570 kb on - strand, within BWI76_RS17855 at 3712.622 kb on + strand, within BWI76_RS17855 at 3712.832 kb on - strand, within BWI76_RS17855 at 3712.936 kb on - strand, within BWI76_RS17855 at 3713.040 kb on + strand, within BWI76_RS17855
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 3,709,953 + BWI76_RS17845 0.72 +0.2 3,709,961 - BWI76_RS17845 0.73 -0.9 3,710,027 - BWI76_RS17845 0.78 +0.0 3,710,108 + BWI76_RS17845 0.84 -0.1 3,710,116 - BWI76_RS17845 0.85 -0.6 3,710,214 + -0.5 3,710,214 + -0.2 3,710,214 + +0.4 3,710,223 + -1.1 3,710,230 - +0.6 3,710,231 - -0.5 3,710,239 + +0.2 3,710,249 + -0.3 3,710,249 + -0.5 3,710,249 + +1.3 3,710,249 + -0.8 3,710,252 - -0.4 3,710,252 - -0.1 3,710,252 - +0.3 3,710,255 + -1.9 3,710,255 + +0.4 3,710,257 - +0.7 3,710,257 - +0.9 3,710,257 - -0.4 3,710,257 - +0.4 3,710,257 - -0.7 3,710,257 - +0.8 3,710,257 - +0.1 3,710,257 - -0.7 3,710,257 - +0.2 3,710,257 - -0.0 3,710,257 - -0.8 3,710,257 - -0.2 3,710,257 - +0.2 3,710,257 - +0.0 3,710,263 - -0.1 3,710,263 - +0.6 3,710,263 - +0.1 3,710,263 - -0.8 3,710,263 - -0.1 3,710,263 - -0.5 3,710,263 - -0.2 3,710,263 - +0.6 3,710,307 + -1.7 3,710,307 + +0.3 3,710,313 - -0.5 3,710,385 + +0.2 3,710,701 + -0.5 3,710,701 + -0.0 3,710,709 - -0.5 3,710,818 + -0.1 3,710,833 - +0.8 3,710,839 + +0.2 3,710,839 + -0.3 3,710,839 + -1.1 3,710,839 + +1.5 3,710,847 - -0.7 3,710,847 - -1.8 3,710,847 - -1.9 3,710,847 - +0.0 3,710,847 - -0.2 3,710,847 - -1.1 3,710,848 - -0.3 3,710,857 - +0.5 3,710,859 + -0.6 3,710,861 + -1.4 3,710,867 - -1.1 3,710,869 - -0.3 3,710,871 + -0.5 3,710,876 + +0.2 3,710,876 + +0.8 3,710,879 - -0.3 3,710,911 - +1.9 3,710,921 - -0.8 3,710,923 + -0.1 3,710,923 - +0.3 3,711,132 + BWI76_RS17850 0.24 +0.8 3,711,133 - BWI76_RS17850 0.24 +0.7 3,711,135 - BWI76_RS17850 0.24 -0.1 3,711,142 + BWI76_RS17850 0.25 -0.9 3,711,308 + BWI76_RS17850 0.38 -0.6 3,711,310 - BWI76_RS17850 0.38 -1.5 3,711,427 - BWI76_RS17850 0.48 +0.3 3,711,460 - BWI76_RS17850 0.50 +1.3 3,711,487 + -0.8 3,711,541 + BWI76_RS17850 0.57 +0.2 3,711,756 + BWI76_RS17850 0.74 -0.5 3,711,831 - BWI76_RS17850 0.80 +0.6 3,711,848 - BWI76_RS17850 0.82 +0.2 3,712,068 - +0.7 3,712,250 - +1.2 3,712,456 + BWI76_RS17855 0.19 +0.5 3,712,484 + BWI76_RS17855 0.20 +0.1 3,712,570 - BWI76_RS17855 0.24 +0.2 3,712,622 + BWI76_RS17855 0.27 -1.4 3,712,832 - BWI76_RS17855 0.37 -0.0 3,712,936 - BWI76_RS17855 0.42 +0.1 3,713,040 + BWI76_RS17855 0.48 -0.3
Or see this region's nucleotide sequence