Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12340 and BWI76_RS12345 are separated by 123 nucleotides BWI76_RS12345 and BWI76_RS12350 overlap by 4 nucleotides
BWI76_RS12340: BWI76_RS12340 - molecular chaperone Tir, at 2,575,395 to 2,576,276
_RS12340
BWI76_RS12345: BWI76_RS12345 - nitrite transporter, at 2,576,400 to 2,576,774
_RS12345
BWI76_RS12350: BWI76_RS12350 - kinase, at 2,576,771 to 2,579,849
_RS12350
Position (kb)
2576
2577 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2575.416 kb on - strand at 2575.424 kb on - strand at 2575.464 kb on - strand at 2575.585 kb on + strand at 2575.702 kb on + strand at 2575.758 kb on + strand, within BWI76_RS12340 at 2575.860 kb on + strand, within BWI76_RS12340 at 2575.861 kb on - strand, within BWI76_RS12340 at 2576.142 kb on + strand, within BWI76_RS12340 at 2576.166 kb on - strand, within BWI76_RS12340 at 2576.168 kb on + strand, within BWI76_RS12340 at 2576.244 kb on + strand at 2576.244 kb on + strand at 2576.249 kb on + strand at 2576.259 kb on + strand at 2576.274 kb on + strand at 2576.274 kb on + strand at 2576.287 kb on + strand at 2576.300 kb on + strand at 2576.300 kb on + strand at 2576.301 kb on - strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.318 kb on - strand at 2576.322 kb on + strand at 2576.322 kb on + strand at 2576.359 kb on + strand at 2576.616 kb on - strand, within BWI76_RS12345 at 2576.656 kb on - strand, within BWI76_RS12345 at 2576.826 kb on + strand at 2576.867 kb on + strand at 2577.120 kb on + strand, within BWI76_RS12350 at 2577.120 kb on + strand, within BWI76_RS12350 at 2577.206 kb on + strand, within BWI76_RS12350 at 2577.206 kb on + strand, within BWI76_RS12350 at 2577.385 kb on - strand, within BWI76_RS12350 at 2577.429 kb on + strand, within BWI76_RS12350 at 2577.473 kb on + strand, within BWI76_RS12350
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 2,575,416 - -1.3 2,575,424 - +0.1 2,575,464 - -0.5 2,575,585 + +0.2 2,575,702 + +2.0 2,575,758 + BWI76_RS12340 0.41 +0.2 2,575,860 + BWI76_RS12340 0.53 -1.3 2,575,861 - BWI76_RS12340 0.53 +0.1 2,576,142 + BWI76_RS12340 0.85 +0.2 2,576,166 - BWI76_RS12340 0.87 -2.2 2,576,168 + BWI76_RS12340 0.88 -0.1 2,576,244 + -1.0 2,576,244 + +0.1 2,576,249 + -0.1 2,576,259 + -0.3 2,576,274 + +0.4 2,576,274 + -1.9 2,576,287 + -0.5 2,576,300 + +0.1 2,576,300 + -0.4 2,576,301 - +1.2 2,576,305 + -0.6 2,576,305 + +0.4 2,576,305 + +0.3 2,576,305 + +0.2 2,576,318 - +1.7 2,576,322 + -0.1 2,576,322 + -1.0 2,576,359 + +0.1 2,576,616 - BWI76_RS12345 0.58 +0.0 2,576,656 - BWI76_RS12345 0.68 -1.4 2,576,826 + -0.4 2,576,867 + -0.1 2,577,120 + BWI76_RS12350 0.11 +0.1 2,577,120 + BWI76_RS12350 0.11 +0.2 2,577,206 + BWI76_RS12350 0.14 +0.1 2,577,206 + BWI76_RS12350 0.14 +0.4 2,577,385 - BWI76_RS12350 0.20 +0.4 2,577,429 + BWI76_RS12350 0.21 -0.8 2,577,473 + BWI76_RS12350 0.23 -1.1
Or see this region's nucleotide sequence