Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10020 and BWI76_RS10025 overlap by 4 nucleotides BWI76_RS10025 and BWI76_RS10030 are separated by 235 nucleotides
BWI76_RS10020: BWI76_RS10020 - ABC transporter, at 2,120,205 to 2,122,352
_RS10020
BWI76_RS10025: BWI76_RS10025 - secretion protein HlyD, at 2,122,349 to 2,123,554
_RS10025
BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933
_RS10030
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-1
0
1 at 2121.562 kb on - strand, within BWI76_RS10020 at 2121.738 kb on + strand, within BWI76_RS10020 at 2121.837 kb on - strand, within BWI76_RS10020 at 2121.847 kb on - strand, within BWI76_RS10020 at 2121.978 kb on + strand, within BWI76_RS10020 at 2121.983 kb on + strand, within BWI76_RS10020 at 2122.252 kb on + strand at 2122.261 kb on + strand at 2122.269 kb on - strand at 2122.590 kb on + strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.888 kb on - strand, within BWI76_RS10025 at 2122.926 kb on + strand, within BWI76_RS10025 at 2122.934 kb on - strand, within BWI76_RS10025 at 2122.941 kb on + strand, within BWI76_RS10025 at 2122.949 kb on - strand, within BWI76_RS10025 at 2123.018 kb on - strand, within BWI76_RS10025 at 2123.023 kb on + strand, within BWI76_RS10025 at 2123.046 kb on - strand, within BWI76_RS10025 at 2123.052 kb on - strand, within BWI76_RS10025 at 2123.057 kb on - strand, within BWI76_RS10025 at 2123.065 kb on - strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.097 kb on - strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.137 kb on + strand, within BWI76_RS10025 at 2123.154 kb on + strand, within BWI76_RS10025 at 2123.164 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.214 kb on - strand, within BWI76_RS10025 at 2123.256 kb on - strand, within BWI76_RS10025 at 2123.324 kb on + strand, within BWI76_RS10025 at 2123.339 kb on - strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.392 kb on + strand, within BWI76_RS10025 at 2123.410 kb on - strand, within BWI76_RS10025 at 2123.456 kb on - strand at 2123.473 kb on + strand at 2123.527 kb on + strand at 2123.535 kb on - strand at 2123.565 kb on + strand at 2123.570 kb on + strand at 2123.570 kb on + strand at 2123.573 kb on - strand at 2123.578 kb on - strand at 2123.578 kb on - strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.613 kb on - strand at 2123.615 kb on + strand at 2123.615 kb on + strand at 2123.618 kb on - strand at 2123.623 kb on - strand at 2123.623 kb on - strand at 2123.665 kb on - strand at 2123.681 kb on - strand at 2123.684 kb on + strand at 2123.692 kb on - strand at 2123.694 kb on - strand at 2123.696 kb on + strand at 2123.704 kb on - strand at 2123.763 kb on - strand at 2123.789 kb on - strand at 2123.791 kb on - strand at 2123.803 kb on + strand at 2123.863 kb on - strand at 2123.864 kb on - strand at 2123.864 kb on - strand at 2123.880 kb on + strand at 2123.966 kb on + strand at 2124.176 kb on + strand, within BWI76_RS10030 at 2124.184 kb on - strand, within BWI76_RS10030 at 2124.367 kb on - strand, within BWI76_RS10030 at 2124.537 kb on - strand, within BWI76_RS10030
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 2,121,562 - BWI76_RS10020 0.63 +0.4 2,121,738 + BWI76_RS10020 0.71 +1.0 2,121,837 - BWI76_RS10020 0.76 +1.1 2,121,847 - BWI76_RS10020 0.76 +0.6 2,121,978 + BWI76_RS10020 0.83 +1.1 2,121,983 + BWI76_RS10020 0.83 +0.6 2,122,252 + +0.2 2,122,261 + +0.5 2,122,269 - +0.7 2,122,590 + BWI76_RS10025 0.20 +0.5 2,122,624 - BWI76_RS10025 0.23 +0.3 2,122,624 - BWI76_RS10025 0.23 +0.8 2,122,888 - BWI76_RS10025 0.45 +0.5 2,122,926 + BWI76_RS10025 0.48 +1.0 2,122,934 - BWI76_RS10025 0.49 +0.4 2,122,941 + BWI76_RS10025 0.49 +0.5 2,122,949 - BWI76_RS10025 0.50 +0.3 2,123,018 - BWI76_RS10025 0.55 +0.8 2,123,023 + BWI76_RS10025 0.56 +0.6 2,123,046 - BWI76_RS10025 0.58 +1.1 2,123,052 - BWI76_RS10025 0.58 +0.7 2,123,057 - BWI76_RS10025 0.59 +0.8 2,123,065 - BWI76_RS10025 0.59 +0.5 2,123,090 + BWI76_RS10025 0.61 -1.0 2,123,090 + BWI76_RS10025 0.61 +1.0 2,123,090 + BWI76_RS10025 0.61 +0.7 2,123,097 - BWI76_RS10025 0.62 +0.3 2,123,111 + BWI76_RS10025 0.63 +0.6 2,123,111 + BWI76_RS10025 0.63 +0.4 2,123,111 + BWI76_RS10025 0.63 +0.8 2,123,119 - BWI76_RS10025 0.64 +0.8 2,123,119 - BWI76_RS10025 0.64 +1.0 2,123,119 - BWI76_RS10025 0.64 +1.2 2,123,137 + BWI76_RS10025 0.65 +1.3 2,123,154 + BWI76_RS10025 0.67 +0.4 2,123,164 + BWI76_RS10025 0.68 +1.6 2,123,169 + BWI76_RS10025 0.68 +0.4 2,123,169 + BWI76_RS10025 0.68 +0.4 2,123,169 + BWI76_RS10025 0.68 +1.2 2,123,169 + BWI76_RS10025 0.68 +0.6 2,123,169 + BWI76_RS10025 0.68 +0.7 2,123,177 - BWI76_RS10025 0.69 +0.4 2,123,177 - BWI76_RS10025 0.69 +0.5 2,123,177 - BWI76_RS10025 0.69 +1.6 2,123,214 - BWI76_RS10025 0.72 +0.0 2,123,256 - BWI76_RS10025 0.75 +1.2 2,123,324 + BWI76_RS10025 0.81 +0.7 2,123,339 - BWI76_RS10025 0.82 +0.8 2,123,348 + BWI76_RS10025 0.83 +1.2 2,123,348 + BWI76_RS10025 0.83 +0.6 2,123,348 + BWI76_RS10025 0.83 +0.7 2,123,356 - BWI76_RS10025 0.83 +0.7 2,123,356 - BWI76_RS10025 0.83 +0.2 2,123,356 - BWI76_RS10025 0.83 -1.3 2,123,392 + BWI76_RS10025 0.86 +1.2 2,123,410 - BWI76_RS10025 0.88 +1.0 2,123,456 - +0.6 2,123,473 + +0.7 2,123,527 + +1.0 2,123,535 - +0.9 2,123,565 + -0.3 2,123,570 + +0.1 2,123,570 + +0.0 2,123,573 - -0.2 2,123,578 - +0.3 2,123,578 - +0.4 2,123,610 + +0.5 2,123,610 + +0.1 2,123,610 + -0.6 2,123,610 + -0.2 2,123,613 - -0.5 2,123,615 + -0.5 2,123,615 + -0.1 2,123,618 - -0.1 2,123,623 - +0.2 2,123,623 - +1.0 2,123,665 - -0.1 2,123,681 - -0.0 2,123,684 + +0.1 2,123,692 - +0.2 2,123,694 - +0.8 2,123,696 + +0.1 2,123,704 - +0.2 2,123,763 - +0.8 2,123,789 - +1.3 2,123,791 - +0.4 2,123,803 + +0.6 2,123,863 - +1.1 2,123,864 - +1.3 2,123,864 - -0.1 2,123,880 + +0.4 2,123,966 + +0.5 2,124,176 + BWI76_RS10030 0.12 -0.0 2,124,184 - BWI76_RS10030 0.13 +0.9 2,124,367 - BWI76_RS10030 0.18 +0.4 2,124,537 - BWI76_RS10030 0.24 +0.9
Or see this region's nucleotide sequence