Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08040

Experiment: Dilute LB (1/20)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08035 and BWI76_RS08040 are separated by 27 nucleotidesBWI76_RS08040 and BWI76_RS08045 are separated by 3 nucleotidesBWI76_RS08045 and BWI76_RS08050 are separated by 17 nucleotidesBWI76_RS08050 and BWI76_RS08055 overlap by 68 nucleotides BWI76_RS08035: BWI76_RS08035 - penicillin-binding protein 2, at 1,703,137 to 1,705,038 _RS08035 BWI76_RS08040: BWI76_RS08040 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,705,066 to 1,705,533 _RS08040 BWI76_RS08045: BWI76_RS08045 - ribosome silencing factor RsfS, at 1,705,537 to 1,705,854 _RS08045 BWI76_RS08050: BWI76_RS08050 - hypothetical protein, at 1,705,872 to 1,706,143 _RS08050 BWI76_RS08055: BWI76_RS08055 - alpha-ribazole phosphatase, at 1,706,076 to 1,706,705 _RS08055 Position (kb) 1705 1706Strain fitness (log2 ratio) -1 0 1at 1705.159 kb on - strand, within BWI76_RS08040at 1705.341 kb on - strand, within BWI76_RS08040at 1706.276 kb on - strand, within BWI76_RS08055

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Per-strain Table

Position Strand Gene LocusTag Fraction Dilute LB (1/20)
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1,705,159 - BWI76_RS08040 0.20 -0.5
1,705,341 - BWI76_RS08040 0.59 -0.8
1,706,276 - BWI76_RS08055 0.32 -0.4

Or see this region's nucleotide sequence