Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06335 and BWI76_RS06340 are separated by 295 nucleotides BWI76_RS06340 and BWI76_RS06345 are separated by 147 nucleotides BWI76_RS06345 and BWI76_RS06350 are separated by 4 nucleotides
BWI76_RS06335: BWI76_RS06335 - nucleoside-specific channel-forming protein Tsx, at 1,333,308 to 1,334,192
_RS06335
BWI76_RS06340: BWI76_RS06340 - hypothetical protein, at 1,334,488 to 1,335,030
_RS06340
BWI76_RS06345: BWI76_RS06345 - transcriptional regulator NrdR, at 1,335,178 to 1,335,627
_RS06345
BWI76_RS06350: BWI76_RS06350 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 1,335,632 to 1,336,735
_RS06350
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-1
0
1 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.544 kb on + strand, within BWI76_RS06335 at 1333.751 kb on - strand, within BWI76_RS06335 at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.154 kb on + strand at 1334.205 kb on + strand at 1334.213 kb on - strand at 1334.217 kb on + strand at 1334.267 kb on - strand at 1334.457 kb on - strand at 1334.573 kb on - strand, within BWI76_RS06340 at 1335.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 1,333,544 + BWI76_RS06335 0.27 -0.5 1,333,544 + BWI76_RS06335 0.27 -0.8 1,333,751 - BWI76_RS06335 0.50 -1.4 1,334,154 + +1.4 1,334,154 + -0.6 1,334,154 + +0.4 1,334,154 + -0.6 1,334,154 + +0.4 1,334,205 + -0.8 1,334,213 - -0.3 1,334,217 + +0.0 1,334,267 - -0.3 1,334,457 - -1.1 1,334,573 - BWI76_RS06340 0.16 -1.2 1,335,621 + -0.1
Or see this region's nucleotide sequence