Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02600 and BWI76_RS02605 are separated by 176 nucleotides BWI76_RS02605 and BWI76_RS02610 overlap by 4 nucleotides BWI76_RS02610 and BWI76_RS02615 are separated by 106 nucleotides
BWI76_RS02600: BWI76_RS02600 - biofilm peroxide resistance protein BsmA, at 532,547 to 532,876
_RS02600
BWI76_RS02605: BWI76_RS02605 - esterase, at 533,053 to 533,769
_RS02605
BWI76_RS02610: BWI76_RS02610 - transcriptional regulator, at 533,766 to 534,521
_RS02610
BWI76_RS02615: BWI76_RS02615 - L-ascorbate-6-phosphate lactonase, at 534,628 to 535,692
_RS02615
Position (kb) 533
534
535 Strain fitness (log2 ratio) -2
-1
0
1 at 532.789 kb on + strand, within BWI76_RS02600 at 532.795 kb on - strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.878 kb on + strand at 532.886 kb on - strand at 533.068 kb on - strand at 533.165 kb on + strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.176 kb on + strand, within BWI76_RS02605 at 533.239 kb on + strand, within BWI76_RS02605 at 533.400 kb on + strand, within BWI76_RS02605 at 533.794 kb on + strand at 533.866 kb on + strand, within BWI76_RS02610 at 533.902 kb on + strand, within BWI76_RS02610 at 533.974 kb on + strand, within BWI76_RS02610 at 533.982 kb on - strand, within BWI76_RS02610 at 534.002 kb on - strand, within BWI76_RS02610 at 534.053 kb on + strand, within BWI76_RS02610 at 534.073 kb on - strand, within BWI76_RS02610 at 534.082 kb on + strand, within BWI76_RS02610 at 534.165 kb on + strand, within BWI76_RS02610 at 534.633 kb on - strand at 534.646 kb on - strand at 534.646 kb on - strand at 534.652 kb on + strand at 534.659 kb on - strand at 534.905 kb on - strand, within BWI76_RS02615 at 535.039 kb on - strand, within BWI76_RS02615 at 535.131 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.156 kb on - strand, within BWI76_RS02615 at 535.240 kb on + strand, within BWI76_RS02615 at 535.241 kb on - strand, within BWI76_RS02615 at 535.291 kb on + strand, within BWI76_RS02615 at 535.299 kb on - strand at 535.415 kb on + strand, within BWI76_RS02615
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 532,789 + BWI76_RS02600 0.73 -0.4 532,795 - BWI76_RS02600 0.75 +0.1 532,834 + BWI76_RS02600 0.87 +0.4 532,834 + BWI76_RS02600 0.87 +1.0 532,834 + BWI76_RS02600 0.87 -1.3 532,842 - BWI76_RS02600 0.89 +0.4 532,842 - BWI76_RS02600 0.89 -0.0 532,842 - BWI76_RS02600 0.89 +0.5 532,878 + -0.5 532,886 - -0.8 533,068 - +0.1 533,165 + BWI76_RS02605 0.16 -0.1 533,173 - BWI76_RS02605 0.17 -1.7 533,173 - BWI76_RS02605 0.17 +0.8 533,173 - BWI76_RS02605 0.17 -0.3 533,176 + BWI76_RS02605 0.17 -0.0 533,239 + BWI76_RS02605 0.26 +1.3 533,400 + BWI76_RS02605 0.48 +0.1 533,794 + -0.5 533,866 + BWI76_RS02610 0.13 -1.1 533,902 + BWI76_RS02610 0.18 -2.2 533,974 + BWI76_RS02610 0.28 -1.8 533,982 - BWI76_RS02610 0.29 -1.0 534,002 - BWI76_RS02610 0.31 -0.9 534,053 + BWI76_RS02610 0.38 -0.9 534,073 - BWI76_RS02610 0.41 +0.3 534,082 + BWI76_RS02610 0.42 -0.7 534,165 + BWI76_RS02610 0.53 +0.9 534,633 - -0.0 534,646 - +1.5 534,646 - +0.2 534,652 + +0.7 534,659 - +0.2 534,905 - BWI76_RS02615 0.26 -0.2 535,039 - BWI76_RS02615 0.39 -0.3 535,131 - BWI76_RS02615 0.47 -1.9 535,134 - BWI76_RS02615 0.48 +1.0 535,134 - BWI76_RS02615 0.48 -0.8 535,156 - BWI76_RS02615 0.50 +0.2 535,240 + BWI76_RS02615 0.57 -1.2 535,241 - BWI76_RS02615 0.58 -0.0 535,291 + BWI76_RS02615 0.62 -0.3 535,299 - -0.0 535,415 + BWI76_RS02615 0.74 -0.6
Or see this region's nucleotide sequence