Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02565 and BWI76_RS02570 are separated by 151 nucleotides BWI76_RS02570 and BWI76_RS02575 are separated by 37 nucleotides BWI76_RS02575 and BWI76_RS02580 are separated by 85 nucleotides BWI76_RS02580 and BWI76_RS02585 are separated by 45 nucleotides
BWI76_RS02565: BWI76_RS02565 - adenylosuccinate synthase, at 524,823 to 526,121
_RS02565
BWI76_RS02570: BWI76_RS02570 - transcriptional regulator, at 526,273 to 526,698
_RS02570
BWI76_RS02575: BWI76_RS02575 - exoribonuclease R, at 526,736 to 529,183
_RS02575
BWI76_RS02580: BWI76_RS02580 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase, at 529,269 to 530,000
_RS02580
BWI76_RS02585: BWI76_RS02585 - hypothetical protein, at 530,046 to 530,399
_RS02585
Position (kb) 526
527
528
529
530 Strain fitness (log2 ratio) -2
-1
0
1
2 at 526.147 kb on - strand at 526.246 kb on + strand at 526.319 kb on + strand, within BWI76_RS02570 at 526.322 kb on - strand, within BWI76_RS02570 at 526.429 kb on + strand, within BWI76_RS02570 at 526.429 kb on + strand, within BWI76_RS02570 at 526.437 kb on - strand, within BWI76_RS02570 at 526.437 kb on - strand, within BWI76_RS02570 at 526.460 kb on + strand, within BWI76_RS02570 at 526.517 kb on + strand, within BWI76_RS02570 at 526.580 kb on + strand, within BWI76_RS02570 at 526.635 kb on + strand, within BWI76_RS02570 at 526.715 kb on + strand at 526.785 kb on - strand at 526.864 kb on + strand at 526.977 kb on + strand at 526.985 kb on - strand, within BWI76_RS02575 at 526.987 kb on + strand, within BWI76_RS02575 at 527.269 kb on - strand, within BWI76_RS02575 at 527.439 kb on + strand, within BWI76_RS02575 at 527.519 kb on + strand, within BWI76_RS02575 at 527.519 kb on + strand, within BWI76_RS02575 at 527.587 kb on - strand, within BWI76_RS02575 at 527.843 kb on - strand, within BWI76_RS02575 at 527.881 kb on - strand, within BWI76_RS02575 at 528.072 kb on + strand, within BWI76_RS02575 at 528.459 kb on + strand, within BWI76_RS02575 at 528.757 kb on - strand, within BWI76_RS02575 at 528.884 kb on - strand, within BWI76_RS02575 at 529.089 kb on - strand at 529.149 kb on - strand at 529.667 kb on + strand, within BWI76_RS02580 at 529.792 kb on + strand, within BWI76_RS02580 at 529.804 kb on + strand, within BWI76_RS02580 at 529.971 kb on - strand at 530.042 kb on - strand at 530.042 kb on - strand at 530.114 kb on - strand, within BWI76_RS02585 at 530.114 kb on - strand, within BWI76_RS02585
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 526,147 - +1.1 526,246 + +0.5 526,319 + BWI76_RS02570 0.11 +0.8 526,322 - BWI76_RS02570 0.12 -0.3 526,429 + BWI76_RS02570 0.37 +0.3 526,429 + BWI76_RS02570 0.37 -0.6 526,437 - BWI76_RS02570 0.38 -1.2 526,437 - BWI76_RS02570 0.38 +0.0 526,460 + BWI76_RS02570 0.44 -0.1 526,517 + BWI76_RS02570 0.57 -0.2 526,580 + BWI76_RS02570 0.72 +0.2 526,635 + BWI76_RS02570 0.85 -0.3 526,715 + +2.1 526,785 - +0.3 526,864 + -0.3 526,977 + -0.0 526,985 - BWI76_RS02575 0.10 -1.3 526,987 + BWI76_RS02575 0.10 -1.8 527,269 - BWI76_RS02575 0.22 -0.7 527,439 + BWI76_RS02575 0.29 -0.9 527,519 + BWI76_RS02575 0.32 -1.0 527,519 + BWI76_RS02575 0.32 -1.4 527,587 - BWI76_RS02575 0.35 -0.4 527,843 - BWI76_RS02575 0.45 +1.6 527,881 - BWI76_RS02575 0.47 -0.8 528,072 + BWI76_RS02575 0.55 +0.0 528,459 + BWI76_RS02575 0.70 +0.1 528,757 - BWI76_RS02575 0.83 +0.4 528,884 - BWI76_RS02575 0.88 -0.3 529,089 - -0.2 529,149 - -0.5 529,667 + BWI76_RS02580 0.54 -1.6 529,792 + BWI76_RS02580 0.71 +0.2 529,804 + BWI76_RS02580 0.73 +1.1 529,971 - +0.0 530,042 - -0.2 530,042 - +0.1 530,114 - BWI76_RS02585 0.19 -0.4 530,114 - BWI76_RS02585 0.19 -0.1
Or see this region's nucleotide sequence