Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01770 and BWI76_RS01775 are separated by 105 nucleotides BWI76_RS01775 and BWI76_RS01780 are separated by 138 nucleotides BWI76_RS01780 and BWI76_RS01785 are separated by 5 nucleotides BWI76_RS01785 and BWI76_RS01790 are separated by 38 nucleotides
BWI76_RS01770: BWI76_RS01770 - transcriptional regulatory protein RtcR, at 361,143 to 362,723
_RS01770
BWI76_RS01775: BWI76_RS01775 - hypothetical protein, at 362,829 to 363,032
_RS01775
BWI76_RS01780: BWI76_RS01780 - SPFH domain / Band 7 family protein, at 363,171 to 364,298
_RS01780
BWI76_RS01785: BWI76_RS01785 - nucleotidyltransferase, at 364,304 to 365,092
_RS01785
BWI76_RS01790: BWI76_RS01790 - lysine-sensitive aspartokinase 3, at 365,131 to 366,480
_RS01790
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2 at 362.224 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.374 kb on - strand, within BWI76_RS01770 at 362.439 kb on + strand at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.584 kb on + strand at 362.632 kb on - strand at 362.689 kb on - strand at 362.916 kb on - strand, within BWI76_RS01775 at 363.055 kb on - strand at 363.055 kb on - strand at 363.055 kb on - strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.240 kb on - strand at 363.272 kb on + strand at 363.374 kb on + strand, within BWI76_RS01780 at 363.377 kb on - strand, within BWI76_RS01780 at 363.419 kb on - strand, within BWI76_RS01780 at 363.521 kb on + strand, within BWI76_RS01780 at 363.617 kb on + strand, within BWI76_RS01780 at 363.635 kb on + strand, within BWI76_RS01780 at 363.974 kb on + strand, within BWI76_RS01780 at 364.030 kb on + strand, within BWI76_RS01780 at 364.049 kb on + strand, within BWI76_RS01780 at 364.122 kb on - strand, within BWI76_RS01780 at 364.145 kb on + strand, within BWI76_RS01780 at 364.211 kb on + strand at 364.216 kb on + strand at 364.216 kb on + strand at 364.454 kb on - strand, within BWI76_RS01785 at 364.515 kb on + strand, within BWI76_RS01785 at 364.854 kb on + strand, within BWI76_RS01785 at 364.923 kb on - strand, within BWI76_RS01785 at 364.928 kb on - strand, within BWI76_RS01785 at 364.986 kb on + strand, within BWI76_RS01785 at 364.996 kb on + strand, within BWI76_RS01785 at 365.004 kb on - strand, within BWI76_RS01785 at 365.065 kb on + strand at 365.273 kb on - strand, within BWI76_RS01790
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 362,224 - BWI76_RS01770 0.68 -1.1 362,307 - BWI76_RS01770 0.74 +0.2 362,307 - BWI76_RS01770 0.74 +0.7 362,374 - BWI76_RS01770 0.78 -0.3 362,439 + +0.6 362,460 - BWI76_RS01770 0.83 +1.8 362,460 - BWI76_RS01770 0.83 -0.0 362,460 - BWI76_RS01770 0.83 -0.4 362,584 + +0.0 362,632 - +0.3 362,689 - -0.1 362,916 - BWI76_RS01775 0.43 -1.0 363,055 - +0.6 363,055 - +0.3 363,055 - +0.5 363,066 + -0.5 363,066 + +1.2 363,066 + +0.5 363,066 + -1.6 363,066 + +0.3 363,074 - -0.8 363,074 - +0.6 363,074 - -0.2 363,074 - +1.2 363,240 - +0.1 363,272 + +0.1 363,374 + BWI76_RS01780 0.18 -0.6 363,377 - BWI76_RS01780 0.18 -1.9 363,419 - BWI76_RS01780 0.22 -0.0 363,521 + BWI76_RS01780 0.31 -0.2 363,617 + BWI76_RS01780 0.40 -0.5 363,635 + BWI76_RS01780 0.41 -0.5 363,974 + BWI76_RS01780 0.71 -0.8 364,030 + BWI76_RS01780 0.76 -1.9 364,049 + BWI76_RS01780 0.78 -1.1 364,122 - BWI76_RS01780 0.84 -1.4 364,145 + BWI76_RS01780 0.86 -0.5 364,211 + -0.1 364,216 + -0.1 364,216 + +1.8 364,454 - BWI76_RS01785 0.19 +0.4 364,515 + BWI76_RS01785 0.27 -0.4 364,854 + BWI76_RS01785 0.70 -0.6 364,923 - BWI76_RS01785 0.78 -0.3 364,928 - BWI76_RS01785 0.79 +0.1 364,986 + BWI76_RS01785 0.86 +0.3 364,996 + BWI76_RS01785 0.88 -0.8 365,004 - BWI76_RS01785 0.89 +0.0 365,065 + -0.2 365,273 - BWI76_RS01790 0.11 +0.2
Or see this region's nucleotide sequence