Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_845 and Pf1N1B4_846 are separated by 232 nucleotides Pf1N1B4_846 and Pf1N1B4_847 are separated by 15 nucleotides
Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533
_845
Pf1N1B4_846 - hypothetical protein, at 974,766 to 974,969
_846
Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664
_847
Position (kb) 974
975 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.862 kb on + strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.935 kb on + strand, within Pf1N1B4_845 at 973.935 kb on + strand at 974.003 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.461 kb on + strand at 974.461 kb on + strand at 974.462 kb on - strand at 974.462 kb on - strand at 974.656 kb on - strand at 974.656 kb on - strand at 974.729 kb on + strand at 974.915 kb on + strand, within Pf1N1B4_846 at 974.915 kb on + strand at 974.916 kb on - strand at 974.922 kb on + strand, within Pf1N1B4_846 at 974.972 kb on + strand at 974.972 kb on + strand at 975.016 kb on - strand at 975.016 kb on - strand at 975.246 kb on - strand, within Pf1N1B4_847 at 975.246 kb on - strand, within Pf1N1B4_847 at 975.326 kb on + strand, within Pf1N1B4_847 at 975.327 kb on - strand, within Pf1N1B4_847 at 975.327 kb on - strand at 975.327 kb on - strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand at 975.861 kb on + strand, within Pf1N1B4_847 at 975.861 kb on + strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand at 975.862 kb on - strand, within Pf1N1B4_847 at 975.963 kb on + strand, within Pf1N1B4_847
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 973,775 + -0.0 973,775 + -0.8 973,775 + -0.0 973,775 + -0.0 973,775 + -0.0 973,775 + -0.0 973,775 + -0.0 973,775 + -0.0 973,775 + -0.0 973,775 + -1.0 973,775 + -0.0 973,775 + -1.4 973,775 + -0.0 973,776 - -0.0 973,776 - -2.8 973,776 - -0.0 973,776 - -0.0 973,776 - -0.0 973,776 - -1.8 973,776 - -1.6 973,776 - -1.9 973,776 - -0.0 973,776 - -1.4 973,862 + Pf1N1B4_845 0.18 -0.0 973,863 - Pf1N1B4_845 0.18 -3.2 973,863 - Pf1N1B4_845 0.18 -0.0 973,935 + Pf1N1B4_845 0.27 -3.8 973,935 + -1.3 974,003 + Pf1N1B4_845 0.35 -0.0 974,215 + Pf1N1B4_845 0.61 -1.3 974,215 + Pf1N1B4_845 0.61 -0.0 974,215 + Pf1N1B4_845 0.61 -0.0 974,216 - Pf1N1B4_845 0.61 -0.0 974,216 - Pf1N1B4_845 0.61 -0.0 974,216 - Pf1N1B4_845 0.61 -1.4 974,216 - Pf1N1B4_845 0.61 +1.6 974,216 - Pf1N1B4_845 0.61 -1.9 974,216 - Pf1N1B4_845 0.61 -0.0 974,304 - Pf1N1B4_845 0.72 -1.3 974,304 - Pf1N1B4_845 0.72 -0.0 974,304 - Pf1N1B4_845 0.72 -1.6 974,368 + Pf1N1B4_845 0.80 -0.0 974,368 + Pf1N1B4_845 0.80 -0.0 974,368 + Pf1N1B4_845 0.80 -0.9 974,369 - Pf1N1B4_845 0.80 -0.0 974,369 - Pf1N1B4_845 0.80 -0.0 974,461 + -0.0 974,461 + -0.0 974,462 - -0.0 974,462 - -0.0 974,656 - -1.0 974,656 - -0.0 974,729 + -0.0 974,915 + Pf1N1B4_846 0.73 -2.8 974,915 + -0.0 974,916 - -0.7 974,922 + Pf1N1B4_846 0.76 -2.4 974,972 + -0.0 974,972 + -2.0 975,016 - -0.4 975,016 - -0.0 975,246 - Pf1N1B4_847 0.16 -0.0 975,246 - Pf1N1B4_847 0.16 -1.6 975,326 + Pf1N1B4_847 0.20 -0.0 975,327 - Pf1N1B4_847 0.20 -0.0 975,327 - -0.0 975,327 - Pf1N1B4_847 0.20 -0.0 975,372 + Pf1N1B4_847 0.23 -0.0 975,372 + Pf1N1B4_847 0.23 -0.0 975,373 - Pf1N1B4_847 0.23 -0.0 975,373 - Pf1N1B4_847 0.23 -1.8 975,373 - -1.7 975,654 + Pf1N1B4_847 0.40 -0.0 975,654 + Pf1N1B4_847 0.40 -3.3 975,654 + Pf1N1B4_847 0.40 -0.0 975,654 + Pf1N1B4_847 0.40 -0.0 975,655 - Pf1N1B4_847 0.40 -1.7 975,655 - Pf1N1B4_847 0.40 -0.0 975,655 - Pf1N1B4_847 0.40 -1.1 975,655 - Pf1N1B4_847 0.40 -0.0 975,655 - Pf1N1B4_847 0.40 -0.0 975,655 - -3.1 975,861 + Pf1N1B4_847 0.52 -0.0 975,861 + Pf1N1B4_847 0.52 -0.0 975,862 - Pf1N1B4_847 0.52 -0.0 975,862 - Pf1N1B4_847 0.52 -0.0 975,862 - +1.0 975,862 - Pf1N1B4_847 0.52 -2.6 975,963 + Pf1N1B4_847 0.58 -0.0
Or see this region's nucleotide sequence