Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_804

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_803 and Pf1N1B4_804 are separated by 144 nucleotidesPf1N1B4_804 and Pf1N1B4_805 are separated by 34 nucleotides Pf1N1B4_803 - Membrane carboxypeptidase (penicillin-binding protein), at 916,810 to 919,224 _803 Pf1N1B4_804 - hypothetical protein, at 919,369 to 921,210 _804 Pf1N1B4_805 - hypothetical protein, at 921,245 to 922,516 _805 Position (kb) 919 920 921 922Strain fitness (log2 ratio) -3 -2 -1 0 1at 918.409 kb on - strand, within Pf1N1B4_803at 918.469 kb on + strand, within Pf1N1B4_803at 918.470 kb on - strand, within Pf1N1B4_803at 918.470 kb on - strand, within Pf1N1B4_803at 918.732 kb on + strand, within Pf1N1B4_803at 918.732 kb on + strand, within Pf1N1B4_803at 918.733 kb on - strand, within Pf1N1B4_803at 919.003 kb on + strandat 919.003 kb on + strandat 919.003 kb on + strandat 919.003 kb on + strandat 919.003 kb on + strandat 919.003 kb on + strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.004 kb on - strandat 919.015 kb on - strandat 919.039 kb on + strandat 919.039 kb on + strandat 919.039 kb on + strandat 919.040 kb on - strandat 919.040 kb on - strandat 919.040 kb on - strandat 919.129 kb on + strandat 919.130 kb on - strandat 919.232 kb on + strandat 919.232 kb on + strandat 919.233 kb on - strandat 919.233 kb on - strandat 919.233 kb on - strandat 919.241 kb on - strandat 919.259 kb on - strandat 919.299 kb on + strandat 919.299 kb on + strandat 919.300 kb on - strandat 919.332 kb on - strandat 919.502 kb on + strandat 919.503 kb on - strandat 919.698 kb on + strand, within Pf1N1B4_804at 919.698 kb on + strand, within Pf1N1B4_804at 919.711 kb on + strand, within Pf1N1B4_804at 919.808 kb on + strand, within Pf1N1B4_804at 920.188 kb on + strandat 920.188 kb on + strand, within Pf1N1B4_804at 920.188 kb on + strand, within Pf1N1B4_804at 920.188 kb on + strand, within Pf1N1B4_804at 920.189 kb on - strand, within Pf1N1B4_804at 920.189 kb on - strand, within Pf1N1B4_804at 920.189 kb on - strand, within Pf1N1B4_804at 920.189 kb on - strand, within Pf1N1B4_804at 920.189 kb on - strand, within Pf1N1B4_804at 920.189 kb on - strandat 920.189 kb on - strand, within Pf1N1B4_804at 920.191 kb on + strandat 920.191 kb on + strand, within Pf1N1B4_804at 920.191 kb on + strand, within Pf1N1B4_804at 920.191 kb on + strand, within Pf1N1B4_804at 920.191 kb on + strand, within Pf1N1B4_804at 920.191 kb on + strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.192 kb on - strand, within Pf1N1B4_804at 920.284 kb on + strand, within Pf1N1B4_804at 920.284 kb on + strand, within Pf1N1B4_804at 920.284 kb on + strand, within Pf1N1B4_804at 920.284 kb on + strand, within Pf1N1B4_804at 920.285 kb on - strand, within Pf1N1B4_804at 920.285 kb on - strand, within Pf1N1B4_804at 920.285 kb on - strand, within Pf1N1B4_804at 920.285 kb on - strand, within Pf1N1B4_804at 920.596 kb on + strand, within Pf1N1B4_804at 920.642 kb on - strand, within Pf1N1B4_804at 920.833 kb on + strand, within Pf1N1B4_804at 920.833 kb on + strand, within Pf1N1B4_804at 920.834 kb on - strand, within Pf1N1B4_804at 920.834 kb on - strand, within Pf1N1B4_804at 920.918 kb on + strand, within Pf1N1B4_804at 920.918 kb on + strand, within Pf1N1B4_804at 920.918 kb on + strand, within Pf1N1B4_804at 920.918 kb on + strand, within Pf1N1B4_804at 920.919 kb on - strand, within Pf1N1B4_804at 920.919 kb on - strand, within Pf1N1B4_804at 920.919 kb on - strandat 920.919 kb on - strand, within Pf1N1B4_804at 920.919 kb on - strandat 920.919 kb on - strand, within Pf1N1B4_804at 921.416 kb on + strand, within Pf1N1B4_805at 921.416 kb on + strand, within Pf1N1B4_805at 921.416 kb on + strand, within Pf1N1B4_805at 921.416 kb on + strand, within Pf1N1B4_805at 921.416 kb on + strand, within Pf1N1B4_805at 921.417 kb on - strand, within Pf1N1B4_805at 921.417 kb on - strand, within Pf1N1B4_805at 921.476 kb on + strand, within Pf1N1B4_805at 921.476 kb on + strand, within Pf1N1B4_805at 921.476 kb on + strand, within Pf1N1B4_805at 921.476 kb on + strand, within Pf1N1B4_805at 921.477 kb on - strand, within Pf1N1B4_805at 921.645 kb on - strand, within Pf1N1B4_805at 921.824 kb on + strand, within Pf1N1B4_805at 921.824 kb on + strand, within Pf1N1B4_805at 921.824 kb on + strand, within Pf1N1B4_805at 921.824 kb on + strand, within Pf1N1B4_805at 921.825 kb on - strand, within Pf1N1B4_805at 921.838 kb on + strand, within Pf1N1B4_805at 921.839 kb on - strand, within Pf1N1B4_805at 922.015 kb on + strand, within Pf1N1B4_805at 922.016 kb on - strand, within Pf1N1B4_805at 922.016 kb on - strand, within Pf1N1B4_805at 922.127 kb on - strand, within Pf1N1B4_805at 922.127 kb on - strand, within Pf1N1B4_805at 922.149 kb on + strand, within Pf1N1B4_805at 922.149 kb on + strand, within Pf1N1B4_805at 922.149 kb on + strand, within Pf1N1B4_805at 922.149 kb on + strand, within Pf1N1B4_805at 922.149 kb on + strand, within Pf1N1B4_805at 922.150 kb on - strand, within Pf1N1B4_805

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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918,409 - Pf1N1B4_803 0.66 -0.3
918,469 + Pf1N1B4_803 0.69 +0.0
918,470 - Pf1N1B4_803 0.69 +0.0
918,470 - Pf1N1B4_803 0.69 -1.8
918,732 + Pf1N1B4_803 0.80 -2.3
918,732 + Pf1N1B4_803 0.80 +0.0
918,733 - Pf1N1B4_803 0.80 -1.3
919,003 + +0.0
919,003 + +0.0
919,003 + +0.0
919,003 + +0.0
919,003 + -1.0
919,003 + +0.0
919,004 - +0.0
919,004 - +0.0
919,004 - +0.0
919,004 - +0.0
919,004 - +0.0
919,004 - +0.0
919,004 - -0.7
919,004 - -2.0
919,004 - +0.0
919,015 - +0.0
919,039 + -1.3
919,039 + +0.0
919,039 + +0.0
919,040 - +0.0
919,040 - +0.0
919,040 - -1.8
919,129 + -2.8
919,130 - +0.0
919,232 + -2.2
919,232 + +1.0
919,233 - -0.3
919,233 - -1.8
919,233 - +0.0
919,241 - -1.9
919,259 - +0.0
919,299 + +0.0
919,299 + +0.0
919,300 - +0.0
919,332 - -1.6
919,502 + -1.8
919,503 - +0.0
919,698 + Pf1N1B4_804 0.18 -1.2
919,698 + Pf1N1B4_804 0.18 +0.0
919,711 + Pf1N1B4_804 0.19 +0.0
919,808 + Pf1N1B4_804 0.24 +0.0
920,188 + +0.0
920,188 + Pf1N1B4_804 0.44 -1.7
920,188 + Pf1N1B4_804 0.44 +0.0
920,188 + Pf1N1B4_804 0.44 +0.0
920,189 - Pf1N1B4_804 0.45 +0.0
920,189 - Pf1N1B4_804 0.45 -0.4
920,189 - Pf1N1B4_804 0.45 +0.0
920,189 - Pf1N1B4_804 0.45 +0.0
920,189 - Pf1N1B4_804 0.45 +0.0
920,189 - +0.0
920,189 - Pf1N1B4_804 0.45 +0.0
920,191 + -0.8
920,191 + Pf1N1B4_804 0.45 +0.0
920,191 + Pf1N1B4_804 0.45 +0.0
920,191 + Pf1N1B4_804 0.45 +0.0
920,191 + Pf1N1B4_804 0.45 +0.0
920,191 + Pf1N1B4_804 0.45 -0.9
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,192 - Pf1N1B4_804 0.45 +0.0
920,284 + Pf1N1B4_804 0.50 +0.0
920,284 + Pf1N1B4_804 0.50 +1.0
920,284 + Pf1N1B4_804 0.50 +0.0
920,284 + Pf1N1B4_804 0.50 +1.0
920,285 - Pf1N1B4_804 0.50 -1.1
920,285 - Pf1N1B4_804 0.50 +1.0
920,285 - Pf1N1B4_804 0.50 +0.0
920,285 - Pf1N1B4_804 0.50 +0.0
920,596 + Pf1N1B4_804 0.67 -1.2
920,642 - Pf1N1B4_804 0.69 +0.0
920,833 + Pf1N1B4_804 0.79 -1.6
920,833 + Pf1N1B4_804 0.79 -2.5
920,834 - Pf1N1B4_804 0.80 -2.0
920,834 - Pf1N1B4_804 0.80 +0.0
920,918 + Pf1N1B4_804 0.84 +0.0
920,918 + Pf1N1B4_804 0.84 +0.1
920,918 + Pf1N1B4_804 0.84 +0.0
920,918 + Pf1N1B4_804 0.84 +0.0
920,919 - Pf1N1B4_804 0.84 -1.7
920,919 - Pf1N1B4_804 0.84 +0.0
920,919 - +0.0
920,919 - Pf1N1B4_804 0.84 -1.8
920,919 - +0.0
920,919 - Pf1N1B4_804 0.84 +0.0
921,416 + Pf1N1B4_805 0.13 +0.0
921,416 + Pf1N1B4_805 0.13 +0.0
921,416 + Pf1N1B4_805 0.13 -1.6
921,416 + Pf1N1B4_805 0.13 +0.0
921,416 + Pf1N1B4_805 0.13 -2.1
921,417 - Pf1N1B4_805 0.14 -1.7
921,417 - Pf1N1B4_805 0.14 -1.7
921,476 + Pf1N1B4_805 0.18 +0.0
921,476 + Pf1N1B4_805 0.18 +0.0
921,476 + Pf1N1B4_805 0.18 -1.7
921,476 + Pf1N1B4_805 0.18 -1.0
921,477 - Pf1N1B4_805 0.18 -1.8
921,645 - Pf1N1B4_805 0.31 -1.5
921,824 + Pf1N1B4_805 0.46 +0.2
921,824 + Pf1N1B4_805 0.46 +0.0
921,824 + Pf1N1B4_805 0.46 -1.7
921,824 + Pf1N1B4_805 0.46 +0.0
921,825 - Pf1N1B4_805 0.46 -0.8
921,838 + Pf1N1B4_805 0.47 +0.0
921,839 - Pf1N1B4_805 0.47 +0.0
922,015 + Pf1N1B4_805 0.61 -2.1
922,016 - Pf1N1B4_805 0.61 +0.0
922,016 - Pf1N1B4_805 0.61 -2.5
922,127 - Pf1N1B4_805 0.69 +0.0
922,127 - Pf1N1B4_805 0.69 +0.0
922,149 + Pf1N1B4_805 0.71 -1.6
922,149 + Pf1N1B4_805 0.71 +0.0
922,149 + Pf1N1B4_805 0.71 +0.0
922,149 + Pf1N1B4_805 0.71 +0.0
922,149 + Pf1N1B4_805 0.71 +0.0
922,150 - Pf1N1B4_805 0.71 -0.9

Or see this region's nucleotide sequence