Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_792

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_790 and Pf1N1B4_791 are separated by 46 nucleotidesPf1N1B4_791 and Pf1N1B4_792 overlap by 37 nucleotidesPf1N1B4_792 and Pf1N1B4_793 overlap by 4 nucleotides Pf1N1B4_790 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 901,799 to 903,673 _790 Pf1N1B4_791 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 903,720 to 904,133 _791 Pf1N1B4_792 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 904,097 to 904,678 _792 Pf1N1B4_793 - sensor histidine kinase, at 904,675 to 906,486 _793 Position (kb) 904 905Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 903.222 kb on + strandat 903.222 kb on + strand, within Pf1N1B4_790at 903.222 kb on + strand, within Pf1N1B4_790at 903.223 kb on - strand, within Pf1N1B4_790at 903.223 kb on - strand, within Pf1N1B4_790at 903.279 kb on + strand, within Pf1N1B4_790at 903.279 kb on + strand, within Pf1N1B4_790at 903.279 kb on + strand, within Pf1N1B4_790at 903.386 kb on + strand, within Pf1N1B4_790at 903.591 kb on - strandat 903.591 kb on - strandat 903.593 kb on + strandat 903.593 kb on + strandat 903.594 kb on - strandat 903.594 kb on - strandat 903.594 kb on - strandat 903.594 kb on - strandat 903.594 kb on - strandat 903.632 kb on + strandat 903.633 kb on - strandat 903.633 kb on - strandat 903.716 kb on + strandat 903.716 kb on + strandat 903.716 kb on + strandat 903.716 kb on + strandat 903.717 kb on - strandat 903.717 kb on - strandat 903.717 kb on - strandat 903.717 kb on - strandat 903.735 kb on + strandat 903.736 kb on - strandat 903.736 kb on - strandat 903.736 kb on - strandat 903.736 kb on - strandat 903.738 kb on + strandat 903.893 kb on + strand, within Pf1N1B4_791at 903.894 kb on - strand, within Pf1N1B4_791at 904.148 kb on + strandat 904.172 kb on + strand, within Pf1N1B4_792at 904.220 kb on + strand, within Pf1N1B4_792at 904.221 kb on - strand, within Pf1N1B4_792at 904.221 kb on - strand, within Pf1N1B4_792at 904.221 kb on - strand, within Pf1N1B4_792at 904.221 kb on - strand, within Pf1N1B4_792at 904.221 kb on - strand, within Pf1N1B4_792at 904.262 kb on + strand, within Pf1N1B4_792at 904.263 kb on - strand, within Pf1N1B4_792at 904.304 kb on + strand, within Pf1N1B4_792at 904.304 kb on + strand, within Pf1N1B4_792at 904.304 kb on + strand, within Pf1N1B4_792at 904.304 kb on + strand, within Pf1N1B4_792at 904.305 kb on - strand, within Pf1N1B4_792at 904.305 kb on - strand, within Pf1N1B4_792at 904.328 kb on + strand, within Pf1N1B4_792at 904.329 kb on - strand, within Pf1N1B4_792at 904.404 kb on - strand, within Pf1N1B4_792at 904.404 kb on - strand, within Pf1N1B4_792at 904.449 kb on - strand, within Pf1N1B4_792at 904.449 kb on - strand, within Pf1N1B4_792at 904.526 kb on + strand, within Pf1N1B4_792at 904.555 kb on - strand, within Pf1N1B4_792at 904.684 kb on + strandat 904.786 kb on + strandat 904.882 kb on + strand, within Pf1N1B4_793at 904.883 kb on - strand, within Pf1N1B4_793at 904.883 kb on - strand, within Pf1N1B4_793at 904.883 kb on - strand, within Pf1N1B4_793at 904.883 kb on - strand, within Pf1N1B4_793at 904.999 kb on + strand, within Pf1N1B4_793at 905.150 kb on + strandat 905.151 kb on - strand, within Pf1N1B4_793at 905.173 kb on + strand, within Pf1N1B4_793at 905.173 kb on + strand, within Pf1N1B4_793at 905.173 kb on + strand, within Pf1N1B4_793at 905.174 kb on - strandat 905.174 kb on - strand, within Pf1N1B4_793at 905.174 kb on - strand, within Pf1N1B4_793at 905.174 kb on - strand, within Pf1N1B4_793at 905.174 kb on - strand, within Pf1N1B4_793at 905.412 kb on + strand, within Pf1N1B4_793at 905.412 kb on + strand, within Pf1N1B4_793at 905.413 kb on - strand, within Pf1N1B4_793at 905.413 kb on - strand, within Pf1N1B4_793at 905.413 kb on - strand, within Pf1N1B4_793at 905.473 kb on + strand, within Pf1N1B4_793at 905.473 kb on + strand, within Pf1N1B4_793at 905.473 kb on + strand, within Pf1N1B4_793at 905.473 kb on + strand, within Pf1N1B4_793at 905.473 kb on + strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strandat 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.474 kb on - strand, within Pf1N1B4_793at 905.483 kb on + strand, within Pf1N1B4_793at 905.515 kb on + strand, within Pf1N1B4_793at 905.515 kb on + strand, within Pf1N1B4_793at 905.515 kb on + strand, within Pf1N1B4_793at 905.516 kb on - strand, within Pf1N1B4_793at 905.516 kb on - strand, within Pf1N1B4_793at 905.516 kb on - strand, within Pf1N1B4_793at 905.516 kb on - strand, within Pf1N1B4_793

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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903,222 + -0.8
903,222 + Pf1N1B4_790 0.76 +0.0
903,222 + Pf1N1B4_790 0.76 -4.1
903,223 - Pf1N1B4_790 0.76 +0.0
903,223 - Pf1N1B4_790 0.76 +0.0
903,279 + Pf1N1B4_790 0.79 -1.5
903,279 + Pf1N1B4_790 0.79 -1.6
903,279 + Pf1N1B4_790 0.79 +0.0
903,386 + Pf1N1B4_790 0.85 +0.0
903,591 - +0.0
903,591 - -2.2
903,593 + +0.0
903,593 + +0.0
903,594 - +0.0
903,594 - +0.0
903,594 - +0.0
903,594 - +0.0
903,594 - +0.0
903,632 + +0.0
903,633 - +0.0
903,633 - -1.5
903,716 + -1.8
903,716 + -2.8
903,716 + -2.7
903,716 + -2.0
903,717 - +0.0
903,717 - -1.6
903,717 - +0.0
903,717 - -0.3
903,735 + +0.0
903,736 - +0.0
903,736 - +0.0
903,736 - -1.8
903,736 - -2.4
903,738 + -1.0
903,893 + Pf1N1B4_791 0.42 +0.0
903,894 - Pf1N1B4_791 0.42 -1.2
904,148 + +0.0
904,172 + Pf1N1B4_792 0.13 +0.0
904,220 + Pf1N1B4_792 0.21 +0.0
904,221 - Pf1N1B4_792 0.21 -0.9
904,221 - Pf1N1B4_792 0.21 -1.4
904,221 - Pf1N1B4_792 0.21 +0.0
904,221 - Pf1N1B4_792 0.21 +0.0
904,221 - Pf1N1B4_792 0.21 +0.2
904,262 + Pf1N1B4_792 0.28 +0.0
904,263 - Pf1N1B4_792 0.29 -1.2
904,304 + Pf1N1B4_792 0.36 +0.0
904,304 + Pf1N1B4_792 0.36 -1.3
904,304 + Pf1N1B4_792 0.36 +0.0
904,304 + Pf1N1B4_792 0.36 +0.0
904,305 - Pf1N1B4_792 0.36 +0.0
904,305 - Pf1N1B4_792 0.36 +0.0
904,328 + Pf1N1B4_792 0.40 -1.2
904,329 - Pf1N1B4_792 0.40 -4.5
904,404 - Pf1N1B4_792 0.53 +0.0
904,404 - Pf1N1B4_792 0.53 +0.2
904,449 - Pf1N1B4_792 0.60 -1.3
904,449 - Pf1N1B4_792 0.60 -1.2
904,526 + Pf1N1B4_792 0.74 +0.0
904,555 - Pf1N1B4_792 0.79 +0.0
904,684 + +0.0
904,786 + +0.0
904,882 + Pf1N1B4_793 0.11 +0.0
904,883 - Pf1N1B4_793 0.11 +0.0
904,883 - Pf1N1B4_793 0.11 -1.1
904,883 - Pf1N1B4_793 0.11 +0.0
904,883 - Pf1N1B4_793 0.11 -4.6
904,999 + Pf1N1B4_793 0.18 +0.0
905,150 + -1.6
905,151 - Pf1N1B4_793 0.26 +0.0
905,173 + Pf1N1B4_793 0.27 +0.0
905,173 + Pf1N1B4_793 0.27 +0.0
905,173 + Pf1N1B4_793 0.27 -1.1
905,174 - +0.0
905,174 - Pf1N1B4_793 0.28 +0.0
905,174 - Pf1N1B4_793 0.28 +0.0
905,174 - Pf1N1B4_793 0.28 +0.0
905,174 - Pf1N1B4_793 0.28 +0.0
905,412 + Pf1N1B4_793 0.41 -2.1
905,412 + Pf1N1B4_793 0.41 -0.9
905,413 - Pf1N1B4_793 0.41 -1.3
905,413 - Pf1N1B4_793 0.41 -1.9
905,413 - Pf1N1B4_793 0.41 +0.0
905,473 + Pf1N1B4_793 0.44 +0.0
905,473 + Pf1N1B4_793 0.44 +0.0
905,473 + Pf1N1B4_793 0.44 +0.0
905,473 + Pf1N1B4_793 0.44 +0.0
905,473 + Pf1N1B4_793 0.44 +0.0
905,474 - Pf1N1B4_793 0.44 +0.0
905,474 - -1.4
905,474 - Pf1N1B4_793 0.44 -2.8
905,474 - Pf1N1B4_793 0.44 +0.0
905,474 - Pf1N1B4_793 0.44 +0.0
905,474 - Pf1N1B4_793 0.44 -2.2
905,474 - Pf1N1B4_793 0.44 -0.6
905,474 - Pf1N1B4_793 0.44 -1.7
905,483 + Pf1N1B4_793 0.45 -2.3
905,515 + Pf1N1B4_793 0.46 -2.0
905,515 + Pf1N1B4_793 0.46 +0.0
905,515 + Pf1N1B4_793 0.46 +0.0
905,516 - Pf1N1B4_793 0.46 +0.0
905,516 - Pf1N1B4_793 0.46 +0.0
905,516 - Pf1N1B4_793 0.46 +0.0
905,516 - Pf1N1B4_793 0.46 +0.0

Or see this region's nucleotide sequence