Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_691

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_690 and Pf1N1B4_691 are separated by 129 nucleotidesPf1N1B4_691 and Pf1N1B4_692 are separated by 15 nucleotidesPf1N1B4_692 and Pf1N1B4_693 overlap by 4 nucleotides Pf1N1B4_690 - Transcriptional regulator, AraC family, at 788,136 to 789,065 _690 Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250 _691 Pf1N1B4_692 - ABC spermidine/putrescine transporter, inner membrane subunit, at 790,266 to 791,114 _692 Pf1N1B4_693 - ABC spermidine/putrescine transporter, inner membrane subunit, at 791,111 to 791,917 _693 Position (kb) 789 790 791Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 788.220 kb on - strandat 788.241 kb on + strand, within Pf1N1B4_690at 788.241 kb on + strand, within Pf1N1B4_690at 788.242 kb on - strandat 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.370 kb on + strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.379 kb on + strand, within Pf1N1B4_690at 788.380 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.563 kb on - strand, within Pf1N1B4_690at 788.781 kb on + strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.886 kb on + strand, within Pf1N1B4_690at 789.003 kb on + strandat 789.003 kb on + strandat 789.130 kb on + strandat 789.130 kb on + strandat 789.131 kb on - strandat 789.282 kb on - strandat 789.328 kb on - strand, within Pf1N1B4_691at 789.328 kb on - strand, within Pf1N1B4_691at 789.552 kb on - strand, within Pf1N1B4_691at 789.552 kb on - strand, within Pf1N1B4_691at 789.586 kb on + strand, within Pf1N1B4_691at 789.587 kb on - strand, within Pf1N1B4_691at 790.025 kb on + strand, within Pf1N1B4_691at 790.044 kb on - strand, within Pf1N1B4_691at 790.044 kb on - strand, within Pf1N1B4_691at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.390 kb on + strand, within Pf1N1B4_692at 790.391 kb on - strand, within Pf1N1B4_692at 790.391 kb on - strand, within Pf1N1B4_692at 790.391 kb on - strand, within Pf1N1B4_692at 790.391 kb on - strand, within Pf1N1B4_692at 790.427 kb on - strand, within Pf1N1B4_692at 790.427 kb on - strand, within Pf1N1B4_692at 790.435 kb on + strand, within Pf1N1B4_692at 790.436 kb on - strand, within Pf1N1B4_692at 790.436 kb on - strand, within Pf1N1B4_692at 790.798 kb on + strand, within Pf1N1B4_692at 790.798 kb on + strand, within Pf1N1B4_692at 790.799 kb on - strandat 790.799 kb on - strand, within Pf1N1B4_692at 790.799 kb on - strand, within Pf1N1B4_692at 790.799 kb on - strand, within Pf1N1B4_692at 790.933 kb on + strandat 790.934 kb on - strand, within Pf1N1B4_692at 790.948 kb on + strand, within Pf1N1B4_692at 790.948 kb on + strand, within Pf1N1B4_692at 790.948 kb on + strand, within Pf1N1B4_692at 790.949 kb on - strand, within Pf1N1B4_692at 790.949 kb on - strand, within Pf1N1B4_692at 790.949 kb on - strand, within Pf1N1B4_692at 791.154 kb on + strandat 791.154 kb on + strandat 791.155 kb on - strandat 791.155 kb on - strandat 791.155 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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788,220 - -0.0
788,241 + Pf1N1B4_690 0.11 -1.5
788,241 + Pf1N1B4_690 0.11 -2.1
788,242 - -0.0
788,242 - Pf1N1B4_690 0.11 -0.0
788,242 - Pf1N1B4_690 0.11 -0.0
788,242 - Pf1N1B4_690 0.11 -0.0
788,242 - Pf1N1B4_690 0.11 -0.9
788,370 + Pf1N1B4_690 0.25 -0.0
788,371 - Pf1N1B4_690 0.25 -1.8
788,371 - Pf1N1B4_690 0.25 -1.2
788,371 - Pf1N1B4_690 0.25 -2.2
788,371 - Pf1N1B4_690 0.25 -1.6
788,371 - Pf1N1B4_690 0.25 -1.2
788,371 - Pf1N1B4_690 0.25 -0.0
788,379 + Pf1N1B4_690 0.26 -0.0
788,380 - Pf1N1B4_690 0.26 -0.0
788,395 - Pf1N1B4_690 0.28 -3.4
788,395 - Pf1N1B4_690 0.28 -0.9
788,395 - Pf1N1B4_690 0.28 -1.2
788,438 - Pf1N1B4_690 0.32 -0.0
788,438 - Pf1N1B4_690 0.32 -0.0
788,438 - Pf1N1B4_690 0.32 -0.0
788,438 - Pf1N1B4_690 0.32 -0.0
788,562 + Pf1N1B4_690 0.46 -0.0
788,562 + Pf1N1B4_690 0.46 -0.0
788,562 + Pf1N1B4_690 0.46 -0.0
788,562 + Pf1N1B4_690 0.46 -0.0
788,563 - Pf1N1B4_690 0.46 -0.0
788,781 + Pf1N1B4_690 0.69 -0.0
788,782 - Pf1N1B4_690 0.69 -0.0
788,782 - Pf1N1B4_690 0.69 -2.5
788,782 - Pf1N1B4_690 0.69 -0.0
788,886 + Pf1N1B4_690 0.81 -3.3
789,003 + -1.7
789,003 + -0.0
789,130 + -4.5
789,130 + -0.0
789,131 - -0.7
789,282 - -0.0
789,328 - Pf1N1B4_691 0.13 -2.6
789,328 - Pf1N1B4_691 0.13 -0.0
789,552 - Pf1N1B4_691 0.34 -0.0
789,552 - Pf1N1B4_691 0.34 -0.0
789,586 + Pf1N1B4_691 0.37 -0.0
789,587 - Pf1N1B4_691 0.37 -0.0
790,025 + Pf1N1B4_691 0.79 -0.0
790,044 - Pf1N1B4_691 0.80 -0.0
790,044 - Pf1N1B4_691 0.80 -2.3
790,390 + Pf1N1B4_692 0.15 -1.6
790,390 + Pf1N1B4_692 0.15 -0.0
790,390 + Pf1N1B4_692 0.15 -0.0
790,390 + Pf1N1B4_692 0.15 -0.0
790,390 + Pf1N1B4_692 0.15 -0.0
790,390 + Pf1N1B4_692 0.15 -0.0
790,390 + Pf1N1B4_692 0.15 -1.7
790,391 - Pf1N1B4_692 0.15 -0.0
790,391 - Pf1N1B4_692 0.15 -0.0
790,391 - Pf1N1B4_692 0.15 -0.0
790,391 - Pf1N1B4_692 0.15 -0.0
790,427 - Pf1N1B4_692 0.19 -1.5
790,427 - Pf1N1B4_692 0.19 -0.0
790,435 + Pf1N1B4_692 0.20 -0.0
790,436 - Pf1N1B4_692 0.20 -0.0
790,436 - Pf1N1B4_692 0.20 -1.9
790,798 + Pf1N1B4_692 0.63 -0.0
790,798 + Pf1N1B4_692 0.63 -0.0
790,799 - -2.3
790,799 - Pf1N1B4_692 0.63 -0.0
790,799 - Pf1N1B4_692 0.63 -2.5
790,799 - Pf1N1B4_692 0.63 -0.0
790,933 + -0.0
790,934 - Pf1N1B4_692 0.79 -0.0
790,948 + Pf1N1B4_692 0.80 +1.0
790,948 + Pf1N1B4_692 0.80 -1.9
790,948 + Pf1N1B4_692 0.80 -0.0
790,949 - Pf1N1B4_692 0.80 -0.0
790,949 - Pf1N1B4_692 0.80 -1.0
790,949 - Pf1N1B4_692 0.80 -0.0
791,154 + -2.4
791,154 + -1.2
791,155 - -0.0
791,155 - -0.0
791,155 - -0.0

Or see this region's nucleotide sequence