Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_68 and Pf1N1B4_69 are separated by 293 nucleotides Pf1N1B4_69 and Pf1N1B4_70 are separated by 388 nucleotides
Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535
_68
Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542
_69
Pf1N1B4_70 - Major facilitator family transporter, at 72,931 to 74,256
_70
Position (kb) 71
72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.044 kb on + strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.045 kb on - strand, within Pf1N1B4_68 at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.566 kb on + strand at 71.567 kb on - strand at 71.569 kb on + strand at 71.569 kb on + strand at 71.570 kb on - strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.722 kb on + strand at 71.862 kb on - strand at 71.871 kb on + strand at 71.872 kb on - strand at 72.028 kb on - strand, within Pf1N1B4_69 at 72.028 kb on - strand, within Pf1N1B4_69 at 72.139 kb on + strand, within Pf1N1B4_69 at 72.140 kb on - strand, within Pf1N1B4_69 at 72.303 kb on + strand, within Pf1N1B4_69 at 72.304 kb on - strand, within Pf1N1B4_69 at 72.577 kb on - strand at 72.648 kb on - strand at 72.749 kb on - strand at 72.915 kb on + strand at 72.916 kb on - strand at 72.916 kb on - strand at 72.916 kb on - strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.025 kb on - strand at 73.025 kb on - strand at 73.262 kb on - strand, within Pf1N1B4_70 at 73.318 kb on + strand at 73.319 kb on - strand, within Pf1N1B4_70 at 73.319 kb on - strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.322 kb on - strand, within Pf1N1B4_70 at 73.392 kb on - strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.471 kb on + strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 71,044 + Pf1N1B4_68 0.64 -1.0 71,044 + Pf1N1B4_68 0.64 -0.0 71,045 - Pf1N1B4_68 0.64 -0.0 71,045 - Pf1N1B4_68 0.64 -0.0 71,566 + -0.0 71,566 + -2.8 71,566 + -0.0 71,566 + -1.6 71,566 + -1.2 71,566 + -0.0 71,566 + -0.0 71,567 - -1.7 71,569 + -2.2 71,569 + -0.0 71,570 - -0.5 71,722 + -1.6 71,722 + -1.7 71,722 + -0.0 71,722 + -1.8 71,862 - -4.3 71,871 + -1.0 71,872 - -2.9 72,028 - Pf1N1B4_69 0.28 -0.0 72,028 - Pf1N1B4_69 0.28 -2.4 72,139 + Pf1N1B4_69 0.43 -0.0 72,140 - Pf1N1B4_69 0.44 -0.0 72,303 + Pf1N1B4_69 0.66 -0.0 72,304 - Pf1N1B4_69 0.67 -0.0 72,577 - -0.0 72,648 - -0.0 72,749 - -0.0 72,915 + -0.0 72,916 - -0.0 72,916 - -1.1 72,916 - -0.0 73,024 + +1.0 73,024 + -0.0 73,024 + -2.2 73,024 + -1.9 73,024 + -0.0 73,024 + -0.0 73,025 - -0.0 73,025 - -0.0 73,262 - Pf1N1B4_70 0.25 -3.1 73,318 + -1.2 73,319 - Pf1N1B4_70 0.29 -0.0 73,319 - Pf1N1B4_70 0.29 -1.1 73,321 + Pf1N1B4_70 0.29 -1.2 73,321 + Pf1N1B4_70 0.29 -0.0 73,322 - Pf1N1B4_70 0.29 -2.9 73,392 - -0.5 73,471 + Pf1N1B4_70 0.41 -0.0 73,471 + -0.0 73,471 + Pf1N1B4_70 0.41 -1.6 73,472 - Pf1N1B4_70 0.41 -2.4 73,472 - Pf1N1B4_70 0.41 -2.0 73,472 - -0.6
Or see this region's nucleotide sequence