Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_675 and Pf1N1B4_676 are separated by 146 nucleotides Pf1N1B4_676 and Pf1N1B4_677 are separated by 222 nucleotides Pf1N1B4_677 and Pf1N1B4_678 are separated by 257 nucleotides
Pf1N1B4_675 - hypothetical protein, at 773,129 to 773,347
_675
Pf1N1B4_676 - FIG00953991: hypothetical protein, at 773,494 to 773,853
_676
Pf1N1B4_677 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 774,076 to 774,390
_677
Pf1N1B4_678 - Pyruvate kinase (EC 2.7.1.40), at 774,648 to 776,099
_678
Position (kb) 773
774 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 772.597 kb on + strand at 772.651 kb on + strand at 772.652 kb on - strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.673 kb on - strand at 772.673 kb on - strand at 772.716 kb on + strand at 772.717 kb on - strand at 772.717 kb on - strand at 772.720 kb on - strand at 772.734 kb on - strand at 772.748 kb on - strand at 772.825 kb on - strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.922 kb on + strand at 773.066 kb on + strand at 773.067 kb on - strand at 773.086 kb on + strand at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.345 kb on + strand at 773.346 kb on - strand at 773.352 kb on + strand at 773.352 kb on + strand at 773.353 kb on - strand at 773.357 kb on - strand at 773.357 kb on - strand at 773.357 kb on - strand at 773.425 kb on + strand at 773.425 kb on + strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.474 kb on + strand at 773.475 kb on - strand at 773.528 kb on + strand at 773.529 kb on - strand at 773.529 kb on - strand at 773.597 kb on + strand, within Pf1N1B4_676 at 773.597 kb on + strand, within Pf1N1B4_676 at 773.597 kb on + strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.698 kb on - strand, within Pf1N1B4_676 at 773.761 kb on - strand, within Pf1N1B4_676 at 773.813 kb on + strand, within Pf1N1B4_676 at 773.896 kb on + strand at 773.897 kb on - strand at 773.918 kb on - strand at 773.918 kb on - strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.926 kb on - strand at 773.926 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.986 kb on + strand at 774.008 kb on + strand at 774.009 kb on - strand at 774.018 kb on + strand at 774.019 kb on - strand at 774.050 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.226 kb on - strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.365 kb on + strand at 774.365 kb on + strand at 774.365 kb on + strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.486 kb on - strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.575 kb on - strand at 774.575 kb on - strand at 774.594 kb on + strand at 774.595 kb on - strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.609 kb on + strand at 774.609 kb on + strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.678 kb on - strand at 774.695 kb on + strand at 774.696 kb on - strand at 774.696 kb on - strand at 774.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 772,597 + -0.0 772,651 + -0.0 772,652 - -0.0 772,672 + -0.0 772,672 + -0.0 772,672 + -0.0 772,673 - -0.0 772,673 - -2.7 772,716 + -0.0 772,717 - -2.5 772,717 - -0.0 772,720 - -1.9 772,734 - -0.0 772,748 - -1.5 772,825 - -1.9 772,852 + -2.1 772,852 + -0.0 772,852 + -2.3 772,852 + -0.0 772,852 + -0.0 772,894 + +0.7 772,894 + -0.0 772,894 + -2.8 772,894 + -0.0 772,894 + -2.2 772,895 - -0.0 772,895 - -0.0 772,895 - -1.8 772,895 - -1.3 772,895 - -3.2 772,922 + -0.0 773,066 + -2.2 773,067 - -1.8 773,086 + -1.6 773,187 - Pf1N1B4_675 0.26 -0.0 773,187 - Pf1N1B4_675 0.26 -1.7 773,187 - Pf1N1B4_675 0.26 -2.2 773,274 - Pf1N1B4_675 0.66 -0.0 773,274 - Pf1N1B4_675 0.66 -0.0 773,274 - Pf1N1B4_675 0.66 -0.0 773,345 + -1.9 773,346 - -0.0 773,352 + -0.0 773,352 + -0.0 773,353 - -0.0 773,357 - -0.0 773,357 - -1.8 773,357 - -2.0 773,425 + -0.0 773,425 + -0.0 773,426 - -1.1 773,426 - -0.0 773,426 - -0.0 773,426 - -0.0 773,426 - -0.0 773,474 + -3.9 773,475 - -0.0 773,528 + -1.4 773,529 - -0.0 773,529 - +1.0 773,597 + Pf1N1B4_676 0.29 -0.0 773,597 + Pf1N1B4_676 0.29 -0.0 773,597 + Pf1N1B4_676 0.29 -2.2 773,598 - Pf1N1B4_676 0.29 -0.0 773,598 - Pf1N1B4_676 0.29 -0.0 773,598 - Pf1N1B4_676 0.29 -2.7 773,698 - Pf1N1B4_676 0.57 -0.0 773,761 - Pf1N1B4_676 0.74 -0.0 773,813 + Pf1N1B4_676 0.89 -0.0 773,896 + -4.2 773,897 - -2.6 773,918 - -1.4 773,918 - -0.0 773,925 + -0.0 773,925 + -0.0 773,925 + -0.0 773,925 + -0.0 773,926 - -0.7 773,926 - -1.2 773,928 - -1.6 773,928 - -1.3 773,928 - -1.3 773,928 - -2.2 773,986 + -1.9 774,008 + -1.1 774,009 - -0.5 774,018 + -0.0 774,019 - -0.0 774,050 + -0.0 774,052 + -0.0 774,052 + -0.0 774,052 + -1.9 774,052 + -0.0 774,052 + -0.0 774,052 + -2.6 774,052 + -0.0 774,052 + -3.7 774,052 + -0.0 774,052 + -2.3 774,052 + -0.0 774,053 - -0.6 774,053 - -1.2 774,053 - -0.0 774,053 - -0.0 774,053 - -0.0 774,053 - -2.7 774,053 - -0.0 774,053 - -0.0 774,053 - -0.0 774,053 - -0.8 774,207 - Pf1N1B4_677 0.42 -1.9 774,207 - Pf1N1B4_677 0.42 -0.0 774,207 - Pf1N1B4_677 0.42 -0.0 774,226 - Pf1N1B4_677 0.48 -2.7 774,284 + Pf1N1B4_677 0.66 -0.0 774,284 + Pf1N1B4_677 0.66 -1.4 774,284 + Pf1N1B4_677 0.66 -0.0 774,284 + Pf1N1B4_677 0.66 -0.3 774,284 + Pf1N1B4_677 0.66 -0.0 774,284 + Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -0.0 774,285 - Pf1N1B4_677 0.66 -1.2 774,285 - Pf1N1B4_677 0.66 -0.0 774,365 + -0.0 774,365 + -0.0 774,365 + -0.0 774,366 - -0.0 774,366 - -0.0 774,366 - -1.1 774,486 - -0.0 774,549 + -0.0 774,549 + -1.1 774,549 + +0.7 774,550 - -0.0 774,550 - -3.8 774,550 - -1.0 774,550 - -0.0 774,550 - -0.0 774,550 - -0.0 774,550 - -2.6 774,575 - -0.0 774,575 - -0.0 774,594 + -3.8 774,595 - -0.0 774,600 + -1.5 774,600 + -4.0 774,600 + -1.6 774,601 - -2.4 774,601 - -4.1 774,601 - -0.0 774,609 + -2.0 774,609 + -0.0 774,610 - -0.0 774,610 - -1.5 774,610 - -2.0 774,610 - -0.0 774,610 - -2.1 774,610 - -0.0 774,612 + -3.0 774,612 + -0.0 774,612 + -0.0 774,613 - -0.0 774,613 - -2.8 774,613 - -1.0 774,613 - -0.0 774,613 - -0.7 774,613 - +0.4 774,643 + -0.0 774,643 + -0.0 774,643 + -3.2 774,643 + -0.0 774,643 + -4.4 774,644 - -2.0 774,644 - -0.0 774,644 - -0.0 774,644 - -0.0 774,644 - -0.0 774,644 - -1.2 774,644 - -0.0 774,678 - -4.6 774,695 + -0.0 774,696 - -1.3 774,696 - -0.0 774,696 - -1.8
Or see this region's nucleotide sequence