Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5632 and Pf1N1B4_5633 are separated by 7 nucleotides Pf1N1B4_5633 and Pf1N1B4_5634 are separated by 140 nucleotides Pf1N1B4_5634 and Pf1N1B4_5635 are separated by 120 nucleotides
Pf1N1B4_5632 - D-beta-hydroxybutyrate permease, at 59,821 to 61,212
_5632
Pf1N1B4_5633 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 61,220 to 61,993
_5633
Pf1N1B4_5634 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 62,134 to 64,089
_5634
Pf1N1B4_5635 - Twitching motility protein PilT, at 64,210 to 65,373
_5635
Position (kb) 62
63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 61.144 kb on - strand at 61.146 kb on + strand at 61.146 kb on + strand at 61.147 kb on - strand at 61.192 kb on + strand at 61.192 kb on + strand at 61.215 kb on + strand at 61.216 kb on - strand at 61.917 kb on + strand at 61.923 kb on + strand at 61.966 kb on + strand at 62.813 kb on - strand at 62.813 kb on - strand, within Pf1N1B4_5634 at 62.832 kb on + strand at 62.835 kb on - strand, within Pf1N1B4_5634 at 62.835 kb on - strand, within Pf1N1B4_5634 at 62.936 kb on - strand, within Pf1N1B4_5634 at 62.944 kb on + strand, within Pf1N1B4_5634 at 63.064 kb on + strand, within Pf1N1B4_5634 at 63.064 kb on + strand at 63.064 kb on + strand, within Pf1N1B4_5634 at 63.067 kb on + strand, within Pf1N1B4_5634 at 63.067 kb on + strand, within Pf1N1B4_5634 at 63.067 kb on + strand, within Pf1N1B4_5634 at 63.340 kb on + strand, within Pf1N1B4_5634 at 63.488 kb on + strand, within Pf1N1B4_5634 at 63.746 kb on - strand, within Pf1N1B4_5634 at 64.161 kb on - strand at 64.161 kb on - strand at 64.242 kb on + strand at 64.243 kb on - strand at 64.545 kb on + strand at 64.943 kb on - strand, within Pf1N1B4_5635 at 65.067 kb on + strand, within Pf1N1B4_5635
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 61,144 - -1.4 61,146 + +0.1 61,146 + +0.1 61,147 - -1.5 61,192 + +0.1 61,192 + +0.1 61,215 + -1.3 61,216 - -2.3 61,917 + +0.1 61,923 + +0.1 61,966 + -0.6 62,813 - -2.7 62,813 - Pf1N1B4_5634 0.35 +0.1 62,832 + +0.1 62,835 - Pf1N1B4_5634 0.36 +0.1 62,835 - Pf1N1B4_5634 0.36 -1.9 62,936 - Pf1N1B4_5634 0.41 +0.1 62,944 + Pf1N1B4_5634 0.41 +0.1 63,064 + Pf1N1B4_5634 0.48 -2.8 63,064 + -2.0 63,064 + Pf1N1B4_5634 0.48 +0.1 63,067 + Pf1N1B4_5634 0.48 -2.0 63,067 + Pf1N1B4_5634 0.48 +0.1 63,067 + Pf1N1B4_5634 0.48 +0.1 63,340 + Pf1N1B4_5634 0.62 +0.1 63,488 + Pf1N1B4_5634 0.69 +0.1 63,746 - Pf1N1B4_5634 0.82 +0.1 64,161 - +0.1 64,161 - -0.7 64,242 + -0.9 64,243 - -1.1 64,545 + -1.4 64,943 - Pf1N1B4_5635 0.63 -2.3 65,067 + Pf1N1B4_5635 0.74 +0.1
Or see this region's nucleotide sequence