Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5629 and Pf1N1B4_5630 are separated by 20 nucleotides Pf1N1B4_5630 and Pf1N1B4_5631 are separated by 15 nucleotides Pf1N1B4_5631 and Pf1N1B4_5632 are separated by 305 nucleotides
Pf1N1B4_5629 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 56,358 to 57,932
_5629
Pf1N1B4_5630 - hypothetical protein, at 57,953 to 58,084
_5630
Pf1N1B4_5631 - Sigma-54 dependent transcriptional regulator, at 58,100 to 59,515
_5631
Pf1N1B4_5632 - D-beta-hydroxybutyrate permease, at 59,821 to 61,212
_5632
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 57.106 kb on + strand, within Pf1N1B4_5629 at 57.107 kb on - strand, within Pf1N1B4_5629 at 57.110 kb on - strand, within Pf1N1B4_5629 at 57.229 kb on - strand, within Pf1N1B4_5629 at 57.512 kb on + strand, within Pf1N1B4_5629 at 57.769 kb on - strand, within Pf1N1B4_5629 at 57.894 kb on + strand at 58.002 kb on - strand, within Pf1N1B4_5630 at 58.078 kb on + strand at 58.078 kb on + strand at 58.079 kb on - strand at 58.082 kb on + strand at 58.082 kb on + strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.142 kb on - strand at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 59.048 kb on + strand, within Pf1N1B4_5631 at 59.251 kb on + strand, within Pf1N1B4_5631 at 59.251 kb on + strand, within Pf1N1B4_5631 at 59.252 kb on - strand, within Pf1N1B4_5631 at 59.474 kb on + strand at 59.475 kb on - strand at 59.525 kb on + strand at 59.525 kb on + strand at 59.526 kb on - strand at 60.454 kb on + strand, within Pf1N1B4_5632
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 57,106 + Pf1N1B4_5629 0.47 -0.9 57,107 - Pf1N1B4_5629 0.48 +0.1 57,110 - Pf1N1B4_5629 0.48 -2.1 57,229 - Pf1N1B4_5629 0.55 -1.0 57,512 + Pf1N1B4_5629 0.73 -1.2 57,769 - Pf1N1B4_5629 0.90 -1.4 57,894 + -1.5 58,002 - Pf1N1B4_5630 0.37 +0.1 58,078 + -1.7 58,078 + +0.1 58,079 - -2.1 58,082 + +0.1 58,082 + +0.1 58,083 - -0.7 58,083 - +0.1 58,083 - -2.7 58,142 - -2.0 58,557 - Pf1N1B4_5631 0.32 -3.4 58,557 - Pf1N1B4_5631 0.32 -2.3 58,557 - Pf1N1B4_5631 0.32 -2.8 58,732 - Pf1N1B4_5631 0.45 +0.1 58,732 - Pf1N1B4_5631 0.45 +0.1 58,732 - Pf1N1B4_5631 0.45 +0.1 59,048 + Pf1N1B4_5631 0.67 +0.1 59,251 + Pf1N1B4_5631 0.81 +0.1 59,251 + Pf1N1B4_5631 0.81 -3.0 59,252 - Pf1N1B4_5631 0.81 +0.1 59,474 + -1.6 59,475 - -1.5 59,525 + +0.1 59,525 + -2.6 59,526 - +0.1 60,454 + Pf1N1B4_5632 0.45 -1.5
Or see this region's nucleotide sequence