Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5511 and Pf1N1B4_5512 are separated by 342 nucleotides Pf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotides Pf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotides Pf1N1B4_5514 and Pf1N1B4_5515 are separated by 50 nucleotides
Pf1N1B4_5511 - hypothetical protein, at 260,474 to 260,755
_5511
Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244
_5512
Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517
_5513
Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636
_5514
Pf1N1B4_5515 - ATP-dependent DNA helicase pcrA (EC 3.6.1.-), at 262,687 to 266,067
_5515
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.691 kb on - strand, within Pf1N1B4_5511 at 260.786 kb on + strand at 260.786 kb on + strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.790 kb on + strand at 260.920 kb on + strand at 260.920 kb on + strand at 261.018 kb on + strand at 261.018 kb on + strand at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.166 kb on - strand, within Pf1N1B4_5512 at 261.222 kb on - strand, within Pf1N1B4_5512 at 261.260 kb on + strand at 261.260 kb on + strand at 261.261 kb on - strand at 261.261 kb on - strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.266 kb on - strand at 261.266 kb on - strand at 261.287 kb on - strand at 261.287 kb on - strand at 261.296 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.468 kb on + strand, within Pf1N1B4_5513 at 261.534 kb on + strand at 261.535 kb on - strand at 261.550 kb on - strand at 261.555 kb on - strand at 261.668 kb on - strand at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.756 kb on + strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.763 kb on - strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.847 kb on + strand at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 262.307 kb on + strand, within Pf1N1B4_5514 at 262.308 kb on - strand, within Pf1N1B4_5514 at 262.326 kb on + strand, within Pf1N1B4_5514 at 262.561 kb on + strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.562 kb on - strand at 262.682 kb on - strand at 262.684 kb on - strand at 262.689 kb on - strand at 262.689 kb on - strand at 262.689 kb on - strand at 262.796 kb on - strand at 262.824 kb on - strand at 262.844 kb on + strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.848 kb on + strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.849 kb on - strand at 262.852 kb on + strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.853 kb on - strand at 262.986 kb on - strand at 263.216 kb on - strand, within Pf1N1B4_5515 at 263.225 kb on + strand, within Pf1N1B4_5515 at 263.506 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.541 kb on - strand, within Pf1N1B4_5515 at 263.573 kb on + strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515 at 263.574 kb on - strand, within Pf1N1B4_5515
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 260,639 + Pf1N1B4_5511 0.59 +0.2 260,639 + Pf1N1B4_5511 0.59 -2.3 260,640 - Pf1N1B4_5511 0.59 +0.2 260,640 - Pf1N1B4_5511 0.59 +0.2 260,691 - Pf1N1B4_5511 0.77 +0.2 260,786 + -1.6 260,786 + -1.4 260,787 - +0.2 260,787 - -1.0 260,787 - -3.2 260,787 - +0.2 260,790 + -0.6 260,920 + -0.8 260,920 + +0.2 261,018 + -1.5 261,018 + -0.1 261,164 - Pf1N1B4_5512 0.45 +0.2 261,164 - Pf1N1B4_5512 0.45 -2.0 261,166 - Pf1N1B4_5512 0.46 +0.2 261,222 - Pf1N1B4_5512 0.84 -2.8 261,260 + -1.1 261,260 + +0.2 261,261 - +1.2 261,261 - +0.2 261,265 + +0.2 261,265 + -0.7 261,266 - +0.2 261,266 - -1.0 261,287 - -0.8 261,287 - +0.2 261,296 - -1.5 261,316 + +0.2 261,316 + +0.2 261,317 - +0.2 261,317 - +0.2 261,321 + +0.2 261,321 + +0.2 261,321 + +0.2 261,322 - +0.2 261,322 - -1.2 261,322 - -1.8 261,322 - +0.2 261,458 + Pf1N1B4_5513 0.70 -1.0 261,458 + Pf1N1B4_5513 0.70 +0.2 261,459 - Pf1N1B4_5513 0.70 +0.2 261,459 - Pf1N1B4_5513 0.70 -1.9 261,459 - Pf1N1B4_5513 0.70 +0.2 261,468 + Pf1N1B4_5513 0.75 -0.9 261,534 + +0.2 261,535 - +0.2 261,550 - +0.2 261,555 - +0.2 261,668 - +0.2 261,712 - Pf1N1B4_5514 0.12 +0.2 261,712 - Pf1N1B4_5514 0.12 +0.2 261,712 - Pf1N1B4_5514 0.12 -2.9 261,712 - Pf1N1B4_5514 0.12 -2.6 261,712 - Pf1N1B4_5514 0.12 +0.2 261,712 - Pf1N1B4_5514 0.12 +0.2 261,712 - Pf1N1B4_5514 0.12 -2.8 261,756 + Pf1N1B4_5514 0.16 -1.1 261,757 - Pf1N1B4_5514 0.16 +0.2 261,757 - Pf1N1B4_5514 0.16 -2.2 261,757 - Pf1N1B4_5514 0.16 -2.4 261,757 - Pf1N1B4_5514 0.16 +0.2 261,757 - Pf1N1B4_5514 0.16 +0.2 261,757 - Pf1N1B4_5514 0.16 -2.4 261,757 - Pf1N1B4_5514 0.16 -1.5 261,763 - Pf1N1B4_5514 0.17 +0.2 261,843 + Pf1N1B4_5514 0.24 +0.2 261,843 + Pf1N1B4_5514 0.24 +0.2 261,844 - Pf1N1B4_5514 0.24 +0.2 261,844 - Pf1N1B4_5514 0.24 -3.2 261,844 - Pf1N1B4_5514 0.24 +0.2 261,844 - Pf1N1B4_5514 0.24 +0.2 261,844 - Pf1N1B4_5514 0.24 +0.2 261,847 + -1.5 261,848 - Pf1N1B4_5514 0.25 +0.2 261,848 - Pf1N1B4_5514 0.25 -0.8 261,848 - Pf1N1B4_5514 0.25 +0.2 261,848 - Pf1N1B4_5514 0.25 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,981 + Pf1N1B4_5514 0.38 +0.2 261,982 - Pf1N1B4_5514 0.38 +0.2 261,982 - Pf1N1B4_5514 0.38 +0.2 261,982 - Pf1N1B4_5514 0.38 +0.2 261,982 - Pf1N1B4_5514 0.38 +0.2 262,307 + Pf1N1B4_5514 0.69 +0.2 262,308 - Pf1N1B4_5514 0.69 +0.2 262,326 + Pf1N1B4_5514 0.70 +0.2 262,561 + -2.6 262,562 - +0.2 262,562 - -2.5 262,562 - +0.2 262,562 - -2.4 262,562 - -2.3 262,562 - -1.1 262,682 - +0.2 262,684 - -0.8 262,689 - +0.2 262,689 - +0.2 262,689 - +0.2 262,796 - -1.2 262,824 - -1.8 262,844 + +0.2 262,845 - -1.3 262,845 - -1.8 262,845 - -3.7 262,845 - -1.8 262,848 + -2.3 262,848 + +0.2 262,848 + +0.2 262,848 + +0.2 262,849 - -1.9 262,849 - +0.2 262,849 - -0.8 262,849 - +0.2 262,849 - -1.4 262,849 - +0.2 262,849 - +0.2 262,849 - -1.0 262,849 - -2.2 262,849 - +0.2 262,849 - -1.6 262,849 - +0.2 262,849 - +0.2 262,852 + +0.2 262,853 - -1.3 262,853 - +0.2 262,853 - +0.2 262,853 - +0.2 262,853 - -1.9 262,853 - -2.5 262,853 - -2.1 262,986 - -1.7 263,216 - Pf1N1B4_5515 0.16 +0.2 263,225 + Pf1N1B4_5515 0.16 +0.2 263,506 - Pf1N1B4_5515 0.24 -1.5 263,541 - Pf1N1B4_5515 0.25 +0.2 263,541 - Pf1N1B4_5515 0.25 +0.2 263,541 - Pf1N1B4_5515 0.25 +0.2 263,541 - +0.2 263,541 - Pf1N1B4_5515 0.25 +0.2 263,541 - Pf1N1B4_5515 0.25 +0.2 263,541 - Pf1N1B4_5515 0.25 +0.2 263,573 + Pf1N1B4_5515 0.26 +0.2 263,574 - Pf1N1B4_5515 0.26 -0.7 263,574 - Pf1N1B4_5515 0.26 +0.2 263,574 - Pf1N1B4_5515 0.26 -1.6
Or see this region's nucleotide sequence