Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5501

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotidesPf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862 _5500 Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413 _5501 Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542 _5502 Position (kb) 251 252Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 250.144 kb on + strand, within Pf1N1B4_5500at 250.145 kb on - strand, within Pf1N1B4_5500at 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strandat 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strandat 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.268 kb on - strand, within Pf1N1B4_5500at 250.365 kb on + strand, within Pf1N1B4_5500at 250.365 kb on + strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.429 kb on + strand, within Pf1N1B4_5500at 250.429 kb on + strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.689 kb on - strand, within Pf1N1B4_5500at 250.689 kb on - strand, within Pf1N1B4_5500at 250.737 kb on - strand, within Pf1N1B4_5500at 250.757 kb on - strandat 250.758 kb on + strand, within Pf1N1B4_5500at 250.758 kb on + strandat 250.758 kb on + strand, within Pf1N1B4_5500at 250.758 kb on + strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.771 kb on - strandat 250.805 kb on - strandat 250.807 kb on + strandat 250.807 kb on + strandat 250.807 kb on + strandat 250.870 kb on + strandat 250.870 kb on + strandat 250.871 kb on - strandat 250.871 kb on - strandat 250.871 kb on - strandat 250.976 kb on + strandat 250.976 kb on + strandat 250.976 kb on + strandat 250.977 kb on - strandat 250.977 kb on - strandat 251.024 kb on + strandat 251.024 kb on + strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.067 kb on + strandat 251.067 kb on + strandat 251.067 kb on + strandat 251.068 kb on - strandat 251.260 kb on + strand, within Pf1N1B4_5501at 251.261 kb on - strand, within Pf1N1B4_5501at 251.261 kb on - strand, within Pf1N1B4_5501at 251.279 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.411 kb on + strandat 251.411 kb on + strandat 251.412 kb on - strandat 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.518 kb on - strand, within Pf1N1B4_5502at 251.518 kb on - strand, within Pf1N1B4_5502at 251.534 kb on + strand, within Pf1N1B4_5502at 251.590 kb on + strandat 251.739 kb on + strand, within Pf1N1B4_5502at 251.739 kb on + strand, within Pf1N1B4_5502at 251.739 kb on + strand, within Pf1N1B4_5502at 251.740 kb on - strand, within Pf1N1B4_5502at 251.798 kb on + strand, within Pf1N1B4_5502at 251.994 kb on + strand, within Pf1N1B4_5502at 251.995 kb on - strand, within Pf1N1B4_5502at 252.043 kb on + strand, within Pf1N1B4_5502at 252.043 kb on + strand, within Pf1N1B4_5502at 252.043 kb on + strand, within Pf1N1B4_5502at 252.043 kb on + strand, within Pf1N1B4_5502at 252.043 kb on + strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strandat 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.044 kb on - strand, within Pf1N1B4_5502at 252.115 kb on - strand, within Pf1N1B4_5502at 252.143 kb on + strand, within Pf1N1B4_5502at 252.144 kb on - strand, within Pf1N1B4_5502at 252.144 kb on - strandat 252.144 kb on - strand, within Pf1N1B4_5502at 252.179 kb on + strand, within Pf1N1B4_5502at 252.180 kb on - strand, within Pf1N1B4_5502at 252.185 kb on + strand, within Pf1N1B4_5502at 252.239 kb on + strand, within Pf1N1B4_5502at 252.274 kb on + strand, within Pf1N1B4_5502at 252.275 kb on - strand, within Pf1N1B4_5502at 252.382 kb on + strand, within Pf1N1B4_5502at 252.383 kb on - strand, within Pf1N1B4_5502

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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250,144 + Pf1N1B4_5500 0.27 +0.2
250,145 - Pf1N1B4_5500 0.27 +0.2
250,224 + Pf1N1B4_5500 0.35 +0.2
250,224 + +0.2
250,224 + Pf1N1B4_5500 0.35 +0.2
250,224 + +1.2
250,224 + Pf1N1B4_5500 0.35 +0.2
250,224 + Pf1N1B4_5500 0.35 -2.3
250,225 - Pf1N1B4_5500 0.36 -1.2
250,225 - Pf1N1B4_5500 0.36 -1.6
250,225 - Pf1N1B4_5500 0.36 +0.2
250,225 - Pf1N1B4_5500 0.36 +0.2
250,225 - Pf1N1B4_5500 0.36 +0.2
250,225 - Pf1N1B4_5500 0.36 +0.2
250,225 - Pf1N1B4_5500 0.36 +0.2
250,268 - Pf1N1B4_5500 0.40 +0.2
250,365 + Pf1N1B4_5500 0.50 +0.2
250,365 + Pf1N1B4_5500 0.50 -1.6
250,366 - Pf1N1B4_5500 0.50 -1.7
250,366 - Pf1N1B4_5500 0.50 -1.8
250,366 - Pf1N1B4_5500 0.50 +0.2
250,366 - Pf1N1B4_5500 0.50 +0.2
250,429 + Pf1N1B4_5500 0.56 +0.2
250,429 + Pf1N1B4_5500 0.56 -2.2
250,430 - Pf1N1B4_5500 0.56 +0.2
250,430 - Pf1N1B4_5500 0.56 -0.9
250,430 - Pf1N1B4_5500 0.56 +0.2
250,430 - Pf1N1B4_5500 0.56 -2.7
250,689 - Pf1N1B4_5500 0.82 +0.2
250,689 - Pf1N1B4_5500 0.82 -1.4
250,737 - Pf1N1B4_5500 0.87 -1.9
250,757 - +0.2
250,758 + Pf1N1B4_5500 0.89 +0.2
250,758 + -2.5
250,758 + Pf1N1B4_5500 0.89 -3.6
250,758 + Pf1N1B4_5500 0.89 -1.2
250,759 - Pf1N1B4_5500 0.89 +0.2
250,759 - Pf1N1B4_5500 0.89 -1.7
250,759 - Pf1N1B4_5500 0.89 -1.7
250,759 - Pf1N1B4_5500 0.89 -1.1
250,759 - Pf1N1B4_5500 0.89 +0.2
250,759 - Pf1N1B4_5500 0.89 +0.2
250,759 - Pf1N1B4_5500 0.89 +0.2
250,759 - Pf1N1B4_5500 0.89 +0.2
250,771 - +0.2
250,805 - +0.2
250,807 + +0.2
250,807 + +0.2
250,807 + -1.7
250,870 + -0.7
250,870 + -1.7
250,871 - +0.2
250,871 - -4.0
250,871 - +0.2
250,976 + +0.2
250,976 + -1.1
250,976 + -1.8
250,977 - -1.0
250,977 - -2.0
251,024 + +0.2
251,024 + +1.2
251,025 - +0.2
251,025 - -2.2
251,025 - +0.2
251,025 - -2.0
251,025 - +0.2
251,025 - -2.1
251,067 + -1.7
251,067 + +0.2
251,067 + -0.3
251,068 - +0.2
251,260 + Pf1N1B4_5501 0.55 +0.2
251,261 - Pf1N1B4_5501 0.55 -3.7
251,261 - Pf1N1B4_5501 0.55 -2.6
251,279 + Pf1N1B4_5501 0.61 -2.2
251,302 + Pf1N1B4_5501 0.67 +0.2
251,302 + Pf1N1B4_5501 0.67 +0.2
251,302 + Pf1N1B4_5501 0.67 +0.2
251,302 + Pf1N1B4_5501 0.67 +0.2
251,303 - Pf1N1B4_5501 0.68 +0.2
251,303 - Pf1N1B4_5501 0.68 -1.9
251,303 - Pf1N1B4_5501 0.68 -1.2
251,411 + -0.2
251,411 + +0.2
251,412 - -0.9
251,517 + Pf1N1B4_5502 0.12 +0.2
251,517 + Pf1N1B4_5502 0.12 -1.7
251,517 + Pf1N1B4_5502 0.12 +0.2
251,517 + Pf1N1B4_5502 0.12 -0.7
251,517 + Pf1N1B4_5502 0.12 -1.2
251,517 + Pf1N1B4_5502 0.12 +0.2
251,518 - Pf1N1B4_5502 0.12 -3.2
251,518 - Pf1N1B4_5502 0.12 -1.6
251,534 + Pf1N1B4_5502 0.13 -1.6
251,590 + -2.4
251,739 + Pf1N1B4_5502 0.31 +0.2
251,739 + Pf1N1B4_5502 0.31 +0.2
251,739 + Pf1N1B4_5502 0.31 +0.2
251,740 - Pf1N1B4_5502 0.31 -1.5
251,798 + Pf1N1B4_5502 0.36 -1.1
251,994 + Pf1N1B4_5502 0.53 +0.2
251,995 - Pf1N1B4_5502 0.53 +0.2
252,043 + Pf1N1B4_5502 0.57 -2.1
252,043 + Pf1N1B4_5502 0.57 -1.3
252,043 + Pf1N1B4_5502 0.57 -3.7
252,043 + Pf1N1B4_5502 0.57 +0.2
252,043 + Pf1N1B4_5502 0.57 +0.2
252,044 - Pf1N1B4_5502 0.57 +0.2
252,044 - Pf1N1B4_5502 0.57 -2.0
252,044 - +0.2
252,044 - Pf1N1B4_5502 0.57 -2.3
252,044 - Pf1N1B4_5502 0.57 +0.2
252,044 - Pf1N1B4_5502 0.57 +0.2
252,044 - Pf1N1B4_5502 0.57 +0.2
252,044 - Pf1N1B4_5502 0.57 -2.2
252,044 - Pf1N1B4_5502 0.57 +0.2
252,115 - Pf1N1B4_5502 0.63 +0.2
252,143 + Pf1N1B4_5502 0.66 +0.2
252,144 - Pf1N1B4_5502 0.66 +0.2
252,144 - +0.2
252,144 - Pf1N1B4_5502 0.66 -0.8
252,179 + Pf1N1B4_5502 0.69 +0.2
252,180 - Pf1N1B4_5502 0.69 -2.2
252,185 + Pf1N1B4_5502 0.69 +0.2
252,239 + Pf1N1B4_5502 0.74 +0.2
252,274 + Pf1N1B4_5502 0.77 +0.2
252,275 - Pf1N1B4_5502 0.77 +0.2
252,382 + Pf1N1B4_5502 0.86 +0.2
252,383 - Pf1N1B4_5502 0.86 -1.2

Or see this region's nucleotide sequence