Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5482 and Pf1N1B4_5483 are separated by 865 nucleotides Pf1N1B4_5483 and Pf1N1B4_5484 are separated by 397 nucleotides
Pf1N1B4_5482 - hypothetical protein, at 232,189 to 232,308
_5482
Pf1N1B4_5483 - hypothetical protein, at 233,174 to 233,398
_5483
Pf1N1B4_5484 - hypothetical protein, at 233,796 to 233,912
_5484
Position (kb) 233
234 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 232.375 kb on - strand at 232.461 kb on - strand at 232.465 kb on - strand at 232.465 kb on - strand at 232.465 kb on - strand at 232.465 kb on - strand at 232.465 kb on - strand at 232.664 kb on + strand at 232.664 kb on + strand at 232.664 kb on + strand at 232.665 kb on - strand at 232.665 kb on - strand at 232.665 kb on - strand at 232.665 kb on - strand at 232.665 kb on - strand at 232.673 kb on + strand at 232.674 kb on - strand at 232.674 kb on - strand at 232.733 kb on + strand at 232.759 kb on + strand at 232.759 kb on + strand at 232.760 kb on - strand at 232.760 kb on - strand at 232.784 kb on + strand at 232.828 kb on + strand at 232.829 kb on - strand at 232.843 kb on - strand at 232.843 kb on - strand at 232.862 kb on - strand at 232.950 kb on + strand at 232.950 kb on + strand at 232.950 kb on + strand at 232.964 kb on + strand at 233.061 kb on - strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.069 kb on + strand at 233.073 kb on + strand at 233.073 kb on + strand at 233.073 kb on + strand at 233.073 kb on + strand at 233.073 kb on + strand at 233.074 kb on - strand at 233.074 kb on - strand at 233.074 kb on - strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.117 kb on + strand at 233.123 kb on + strand at 233.123 kb on + strand at 233.123 kb on + strand at 233.123 kb on + strand at 233.123 kb on + strand at 233.124 kb on - strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.165 kb on + strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.166 kb on - strand at 233.277 kb on + strand, within Pf1N1B4_5483 at 233.277 kb on + strand, within Pf1N1B4_5483 at 233.277 kb on + strand at 233.277 kb on + strand, within Pf1N1B4_5483 at 233.278 kb on - strand, within Pf1N1B4_5483 at 233.351 kb on + strand, within Pf1N1B4_5483 at 233.417 kb on + strand at 233.462 kb on + strand at 233.462 kb on + strand at 233.462 kb on + strand at 233.467 kb on + strand at 233.467 kb on + strand at 233.467 kb on + strand at 233.468 kb on - strand at 233.558 kb on - strand at 233.657 kb on - strand at 233.657 kb on - strand at 233.657 kb on - strand at 233.657 kb on - strand at 233.657 kb on - strand at 233.665 kb on + strand at 233.665 kb on + strand at 233.666 kb on - strand at 233.741 kb on + strand at 233.741 kb on + strand at 233.741 kb on + strand at 233.741 kb on + strand at 233.741 kb on + strand at 233.742 kb on - strand at 233.742 kb on - strand at 233.742 kb on - strand at 233.782 kb on + strand at 233.782 kb on + strand at 233.782 kb on + strand at 233.782 kb on + strand at 233.782 kb on + strand at 233.782 kb on + strand at 233.783 kb on - strand at 233.783 kb on - strand at 233.783 kb on - strand at 233.786 kb on + strand at 233.786 kb on + strand at 233.786 kb on + strand at 233.786 kb on + strand at 233.787 kb on - strand at 233.787 kb on - strand at 233.787 kb on - strand at 233.909 kb on - strand at 234.021 kb on - strand at 234.114 kb on + strand at 234.115 kb on - strand at 234.115 kb on - strand at 234.115 kb on - strand at 234.121 kb on + strand at 234.121 kb on + strand at 234.121 kb on + strand at 234.122 kb on - strand at 234.122 kb on - strand at 234.159 kb on - strand at 234.168 kb on + strand at 234.169 kb on - strand at 234.169 kb on - strand at 234.233 kb on + strand at 234.394 kb on + strand at 234.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 232,375 - -2.3 232,461 - -1.8 232,465 - +0.2 232,465 - +0.2 232,465 - -1.3 232,465 - -1.9 232,465 - +0.2 232,664 + -0.7 232,664 + +0.2 232,664 + +0.2 232,665 - -1.2 232,665 - +0.2 232,665 - +0.2 232,665 - +0.2 232,665 - -0.2 232,673 + -0.6 232,674 - -1.6 232,674 - +0.2 232,733 + +0.2 232,759 + +0.2 232,759 + -1.0 232,760 - +0.2 232,760 - +0.2 232,784 + -2.4 232,828 + +0.2 232,829 - +0.2 232,843 - +0.2 232,843 - +0.2 232,862 - -1.8 232,950 + -2.1 232,950 + +0.2 232,950 + +0.2 232,964 + +0.2 233,061 - -2.0 233,069 + +0.2 233,069 + +0.2 233,069 + +0.2 233,069 + +0.2 233,069 + -1.0 233,069 + +0.2 233,069 + +0.1 233,069 + -2.7 233,073 + +0.2 233,073 + +0.2 233,073 + +0.2 233,073 + -2.1 233,073 + +0.2 233,074 - -0.7 233,074 - +0.2 233,074 - -1.8 233,117 + -1.2 233,117 + +0.2 233,117 + +0.2 233,117 + +0.2 233,117 + +0.2 233,117 + +0.2 233,117 + -2.0 233,117 + -0.5 233,117 + +0.2 233,117 + -1.6 233,117 + +0.2 233,117 + +0.2 233,123 + -1.6 233,123 + +0.2 233,123 + +0.2 233,123 + +0.2 233,123 + +0.2 233,124 - -2.0 233,165 + +0.2 233,165 + -1.6 233,165 + +0.2 233,165 + +0.2 233,165 + -3.4 233,165 + -2.4 233,165 + -2.1 233,165 + +0.2 233,165 + -1.1 233,165 + -2.7 233,165 + -2.3 233,165 + +0.2 233,165 + +0.2 233,165 + +0.2 233,165 + +0.2 233,165 + +0.2 233,165 + -0.8 233,165 + -2.4 233,165 + +0.2 233,166 - -1.8 233,166 - +0.2 233,166 - +0.2 233,166 - +0.2 233,166 - +0.2 233,166 - +0.2 233,166 - +0.2 233,166 - +0.2 233,166 - -1.6 233,277 + Pf1N1B4_5483 0.46 -1.5 233,277 + Pf1N1B4_5483 0.46 +0.2 233,277 + +0.2 233,277 + Pf1N1B4_5483 0.46 -2.7 233,278 - Pf1N1B4_5483 0.46 +0.2 233,351 + Pf1N1B4_5483 0.79 -4.1 233,417 + +0.2 233,462 + -2.0 233,462 + -1.6 233,462 + +0.2 233,467 + -2.0 233,467 + +0.2 233,467 + -1.3 233,468 - +0.2 233,558 - -2.5 233,657 - +0.2 233,657 - +1.2 233,657 - +0.2 233,657 - -1.4 233,657 - +0.2 233,665 + -2.3 233,665 + +0.2 233,666 - +0.2 233,741 + -0.8 233,741 + +0.2 233,741 + +0.2 233,741 + +0.2 233,741 + +0.2 233,742 - -1.9 233,742 - +0.2 233,742 - -1.4 233,782 + +0.2 233,782 + +0.2 233,782 + -0.7 233,782 + -2.1 233,782 + -1.8 233,782 + +0.2 233,783 - -0.9 233,783 - +0.2 233,783 - +0.2 233,786 + +0.2 233,786 + +0.2 233,786 + -4.5 233,786 + +0.2 233,787 - +0.2 233,787 - -1.0 233,787 - +0.2 233,909 - +0.2 234,021 - -1.8 234,114 + +0.2 234,115 - -1.9 234,115 - -1.8 234,115 - -2.3 234,121 + +0.2 234,121 + -2.2 234,121 + +0.2 234,122 - -2.2 234,122 - +0.2 234,159 - +0.2 234,168 + -2.1 234,169 - +0.2 234,169 - +0.2 234,233 + -1.0 234,394 + +0.2 234,398 - +0.2
Or see this region's nucleotide sequence