Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5436 and Pf1N1B4_5437 are separated by 778 nucleotides
Pf1N1B4_5436 - Probable helicase, at 176,691 to 178,637
_5436
Pf1N1B4_5437 - hypothetical protein, at 179,416 to 179,595
_5437
Position (kb) 179
180 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 178.456 kb on + strand at 178.457 kb on - strand at 178.457 kb on - strand at 178.457 kb on - strand at 178.529 kb on + strand at 178.557 kb on + strand at 178.557 kb on + strand at 178.558 kb on - strand at 178.558 kb on - strand at 178.592 kb on + strand at 178.627 kb on + strand at 178.627 kb on + strand at 178.627 kb on + strand at 178.627 kb on + strand at 178.628 kb on - strand at 178.721 kb on + strand at 178.771 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.775 kb on + strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.776 kb on - strand at 178.779 kb on + strand at 178.779 kb on + strand at 178.779 kb on + strand at 178.779 kb on + strand at 178.831 kb on + strand at 178.831 kb on + strand at 178.831 kb on + strand at 178.855 kb on + strand at 178.895 kb on + strand at 178.895 kb on + strand at 178.895 kb on + strand at 178.896 kb on - strand at 178.896 kb on - strand at 178.899 kb on + strand at 178.899 kb on + strand at 178.900 kb on - strand at 178.902 kb on + strand at 178.902 kb on + strand at 178.902 kb on + strand at 178.903 kb on - strand at 178.903 kb on - strand at 178.975 kb on + strand at 178.988 kb on + strand at 178.988 kb on + strand at 179.040 kb on + strand at 179.040 kb on + strand at 179.040 kb on + strand at 179.041 kb on - strand at 179.041 kb on - strand at 179.123 kb on + strand at 179.129 kb on + strand at 179.129 kb on + strand at 179.183 kb on - strand at 179.236 kb on + strand at 179.270 kb on - strand at 179.270 kb on - strand at 179.273 kb on + strand at 179.274 kb on - strand at 179.314 kb on + strand at 179.338 kb on - strand at 179.355 kb on - strand at 179.394 kb on + strand at 179.443 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.521 kb on + strand, within Pf1N1B4_5437 at 179.522 kb on - strand, within Pf1N1B4_5437 at 179.538 kb on + strand, within Pf1N1B4_5437 at 179.538 kb on + strand, within Pf1N1B4_5437 at 179.538 kb on + strand, within Pf1N1B4_5437 at 179.538 kb on + strand, within Pf1N1B4_5437 at 179.538 kb on + strand, within Pf1N1B4_5437 at 179.539 kb on - strand, within Pf1N1B4_5437 at 179.539 kb on - strand, within Pf1N1B4_5437 at 179.539 kb on - strand, within Pf1N1B4_5437 at 179.557 kb on - strand, within Pf1N1B4_5437 at 179.593 kb on + strand at 179.593 kb on + strand at 179.593 kb on + strand at 179.594 kb on - strand at 179.601 kb on + strand at 179.602 kb on - strand at 179.648 kb on + strand at 179.648 kb on + strand at 179.721 kb on + strand at 179.722 kb on - strand at 179.857 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.896 kb on + strand at 179.897 kb on - strand at 179.897 kb on - strand at 179.897 kb on - strand at 179.897 kb on - strand at 179.898 kb on + strand at 179.970 kb on + strand at 180.042 kb on - strand at 180.071 kb on - strand at 180.199 kb on + strand at 180.199 kb on + strand at 180.206 kb on - strand at 180.206 kb on - strand at 180.332 kb on + strand at 180.333 kb on - strand at 180.375 kb on + strand at 180.376 kb on - strand at 180.414 kb on + strand at 180.414 kb on + strand at 180.415 kb on - strand at 180.453 kb on + strand at 180.453 kb on + strand at 180.453 kb on + strand at 180.453 kb on + strand at 180.453 kb on + strand at 180.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 178,456 + +0.2 178,457 - +0.2 178,457 - -0.7 178,457 - +0.2 178,529 + +0.2 178,557 + +0.2 178,557 + +0.2 178,558 - -1.5 178,558 - -0.9 178,592 + +0.2 178,627 + -2.4 178,627 + +0.2 178,627 + +0.2 178,627 + -2.0 178,628 - -3.9 178,721 + -1.5 178,771 + -2.0 178,775 + -0.5 178,775 + +0.2 178,775 + +0.2 178,775 + +0.2 178,775 + +0.2 178,775 + +0.2 178,775 + +0.2 178,775 + +0.2 178,775 + -2.0 178,775 + +0.2 178,775 + +0.2 178,775 + -1.1 178,775 + -2.1 178,775 + +0.2 178,775 + +0.2 178,775 + -2.2 178,775 + +0.1 178,775 + -2.9 178,775 + +0.2 178,776 - +0.2 178,776 - +0.2 178,776 - -2.4 178,776 - -1.1 178,776 - +0.2 178,776 - +0.2 178,776 - -2.5 178,779 + +0.2 178,779 + +0.2 178,779 + +0.2 178,779 + +0.2 178,831 + +0.2 178,831 + -1.6 178,831 + +0.2 178,855 + -2.4 178,895 + +0.2 178,895 + +0.2 178,895 + +0.2 178,896 - -3.0 178,896 - +0.2 178,899 + +0.2 178,899 + +0.2 178,900 - -2.7 178,902 + +0.2 178,902 + +0.2 178,902 + +0.2 178,903 - -2.0 178,903 - +0.2 178,975 + +0.2 178,988 + +0.2 178,988 + -1.6 179,040 + +0.2 179,040 + -2.0 179,040 + -2.3 179,041 - +0.2 179,041 - +0.2 179,123 + +0.2 179,129 + -2.1 179,129 + -1.6 179,183 - -2.0 179,236 + -2.2 179,270 - +0.2 179,270 - -2.9 179,273 + -1.5 179,274 - -1.9 179,314 + -1.4 179,338 - +0.2 179,355 - +0.2 179,394 + +0.2 179,443 + Pf1N1B4_5437 0.15 +0.2 179,521 + -1.3 179,521 + Pf1N1B4_5437 0.58 -4.3 179,521 + Pf1N1B4_5437 0.58 +0.2 179,521 + Pf1N1B4_5437 0.58 +0.2 179,521 + Pf1N1B4_5437 0.58 -2.9 179,521 + Pf1N1B4_5437 0.58 +1.2 179,521 + Pf1N1B4_5437 0.58 +0.2 179,522 - Pf1N1B4_5437 0.59 -3.1 179,538 + Pf1N1B4_5437 0.68 +0.2 179,538 + Pf1N1B4_5437 0.68 -1.9 179,538 + Pf1N1B4_5437 0.68 -2.2 179,538 + Pf1N1B4_5437 0.68 +0.2 179,538 + Pf1N1B4_5437 0.68 +0.2 179,539 - Pf1N1B4_5437 0.68 +0.2 179,539 - Pf1N1B4_5437 0.68 -2.2 179,539 - Pf1N1B4_5437 0.68 -1.2 179,557 - Pf1N1B4_5437 0.78 +0.2 179,593 + +0.2 179,593 + -3.2 179,593 + -0.8 179,594 - -3.1 179,601 + -1.7 179,602 - +0.2 179,648 + -1.6 179,648 + +0.2 179,721 + +0.2 179,722 - -1.5 179,857 + +0.2 179,896 + -2.0 179,896 + +0.2 179,896 + -0.5 179,896 + +0.2 179,896 + +0.2 179,896 + +0.2 179,896 + +0.2 179,896 + -1.2 179,896 + -1.7 179,896 + -1.0 179,896 + -2.4 179,896 + +0.2 179,896 + -1.1 179,896 + -1.8 179,896 + +0.2 179,896 + +0.2 179,896 + +0.2 179,896 + +0.2 179,896 + +0.2 179,897 - +0.2 179,897 - +0.2 179,897 - -2.1 179,897 - -2.1 179,898 + -1.9 179,970 + -1.5 180,042 - -1.2 180,071 - +0.2 180,199 + -0.8 180,199 + -1.7 180,206 - -3.1 180,206 - +0.2 180,332 + +0.2 180,333 - +0.2 180,375 + +0.2 180,376 - +0.2 180,414 + -1.6 180,414 + -1.0 180,415 - -2.5 180,453 + +0.2 180,453 + +0.2 180,453 + -2.3 180,453 + -1.9 180,453 + -1.9 180,512 + +0.2
Or see this region's nucleotide sequence