Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5432 and Pf1N1B4_5433 are separated by 907 nucleotides Pf1N1B4_5433 and Pf1N1B4_5434 are separated by 868 nucleotides
Pf1N1B4_5432 - hypothetical protein, at 171,160 to 171,348
_5432
Pf1N1B4_5433 - hypothetical protein, at 172,256 to 172,825
_5433
Pf1N1B4_5434 - HIPA PROTEIN, at 173,694 to 175,007
_5434
Position (kb) 172
173 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.365 kb on - strand at 171.416 kb on + strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.561 kb on - strand at 171.585 kb on - strand at 171.667 kb on - strand at 171.705 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.734 kb on - strand at 171.756 kb on - strand at 171.761 kb on + strand at 171.762 kb on - strand at 171.762 kb on - strand at 171.849 kb on - strand at 171.855 kb on + strand at 171.887 kb on - strand at 171.892 kb on + strand at 171.954 kb on + strand at 171.954 kb on + strand at 171.965 kb on + strand at 171.966 kb on - strand at 172.073 kb on - strand at 172.108 kb on + strand at 172.243 kb on + strand at 172.243 kb on + strand at 172.243 kb on + strand at 172.244 kb on - strand at 172.255 kb on + strand at 172.255 kb on + strand at 172.316 kb on + strand, within Pf1N1B4_5433 at 172.494 kb on + strand, within Pf1N1B4_5433 at 172.495 kb on - strand at 172.515 kb on + strand, within Pf1N1B4_5433 at 172.515 kb on + strand, within Pf1N1B4_5433 at 172.753 kb on + strand, within Pf1N1B4_5433 at 172.753 kb on + strand, within Pf1N1B4_5433 at 172.890 kb on + strand at 173.004 kb on - strand at 173.004 kb on - strand at 173.110 kb on + strand at 173.196 kb on + strand at 173.196 kb on + strand at 173.196 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.275 kb on - strand at 173.275 kb on - strand at 173.275 kb on - strand at 173.276 kb on + strand at 173.276 kb on + strand at 173.276 kb on + strand at 173.277 kb on - strand at 173.285 kb on + strand at 173.285 kb on + strand at 173.286 kb on - strand at 173.389 kb on - strand at 173.584 kb on - strand at 173.584 kb on - strand at 173.617 kb on - strand at 173.617 kb on - strand at 173.664 kb on + strand at 173.665 kb on - strand at 173.665 kb on - strand at 173.665 kb on - strand at 173.686 kb on - strand at 173.699 kb on - strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.784 kb on + strand at 173.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 171,291 - Pf1N1B4_5432 0.69 +0.2 171,291 - Pf1N1B4_5432 0.69 +0.2 171,365 - +0.2 171,416 + -2.6 171,417 - -1.2 171,417 - -2.5 171,417 - +0.2 171,417 - +0.2 171,417 - +0.2 171,561 - -2.2 171,585 - -1.3 171,667 - -1.1 171,705 + -1.2 171,729 + -1.4 171,729 + -1.7 171,729 + +0.2 171,729 + +0.2 171,730 - +0.2 171,730 - -2.1 171,730 - +0.2 171,730 - -1.3 171,733 + +0.2 171,733 + +0.2 171,733 + +0.2 171,734 - -1.4 171,756 - +0.2 171,761 + +0.2 171,762 - +0.2 171,762 - +0.2 171,849 - -1.4 171,855 + +0.2 171,887 - +0.2 171,892 + +0.2 171,954 + +0.2 171,954 + +0.2 171,965 + +0.2 171,966 - -2.6 172,073 - +0.2 172,108 + +0.2 172,243 + +0.2 172,243 + -0.5 172,243 + +0.2 172,244 - -2.1 172,255 + -1.6 172,255 + -0.7 172,316 + Pf1N1B4_5433 0.11 +0.2 172,494 + Pf1N1B4_5433 0.42 -2.4 172,495 - +0.2 172,515 + Pf1N1B4_5433 0.45 -3.4 172,515 + Pf1N1B4_5433 0.45 -1.3 172,753 + Pf1N1B4_5433 0.87 +0.2 172,753 + Pf1N1B4_5433 0.87 -2.9 172,890 + -1.8 173,004 - +0.2 173,004 - -1.7 173,110 + +0.2 173,196 + +0.2 173,196 + +0.2 173,196 + +0.2 173,274 + +0.2 173,274 + -1.4 173,274 + +0.2 173,274 + -0.8 173,274 + -1.5 173,274 + +0.2 173,274 + +0.2 173,274 + -2.5 173,274 + +0.2 173,274 + +0.2 173,274 + +0.2 173,274 + -1.2 173,275 - -1.2 173,275 - +0.2 173,275 - +0.2 173,276 + -2.4 173,276 + +0.2 173,276 + +0.2 173,277 - -2.1 173,285 + +0.2 173,285 + -0.9 173,286 - +0.2 173,389 - +0.2 173,584 - +0.2 173,584 - +0.2 173,617 - +0.2 173,617 - +0.2 173,664 + +0.2 173,665 - +0.2 173,665 - -2.0 173,665 - +0.2 173,686 - +0.2 173,699 - -2.2 173,716 + +0.2 173,716 + +0.2 173,716 + +0.2 173,716 + -2.2 173,717 - +0.2 173,717 - +0.2 173,717 - +0.2 173,717 - -1.2 173,717 - -1.4 173,784 + -2.9 173,784 + -1.2
Or see this region's nucleotide sequence