Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_5338 and Pf1N1B4_5339 are separated by 106 nucleotides Pf1N1B4_5339 and Pf1N1B4_5340 are separated by 608 nucleotides Pf1N1B4_5340 and Pf1N1B4_5341 are separated by 357 nucleotides Pf1N1B4_5341 and Pf1N1B4_5342 are separated by 109 nucleotides Pf1N1B4_5342 and Pf1N1B4_5343 are separated by 88 nucleotides  
        
        Pf1N1B4_5338 - hypothetical protein, at 82,893 to 83,111 
         
        _5338 
         
        
        Pf1N1B4_5339 - hypothetical protein, at 83,218 to 83,340 
         
        _5339 
         
        
        Pf1N1B4_5340 - hypothetical protein, at 83,949 to 84,311 
         
        _5340 
         
        
        Pf1N1B4_5341 - hypothetical protein, at 84,669 to 84,830 
         
        _5341 
         
        
        Pf1N1B4_5342 - hypothetical protein, at 84,940 to 85,110 
         
        _5342 
         
        
        Pf1N1B4_5343 - hypothetical protein, at 85,199 to 85,933 
         
        _5343 
         Position (kb)  
83 
 
84 
 
85 Strain fitness (log2 ratio)  
-4 
 
-3 
 
-2 
 
-1 
 
0 
 
1 at 83.011 kb on + strand, within Pf1N1B4_5338 at 83.011 kb on + strand, within Pf1N1B4_5338 at 83.012 kb on - strand, within Pf1N1B4_5338 at 83.012 kb on - strand, within Pf1N1B4_5338 at 83.186 kb on + strand at 83.397 kb on + strand at 83.398 kb on - strand at 83.513 kb on + strand at 83.513 kb on + strand at 83.514 kb on - strand at 83.514 kb on - strand at 83.514 kb on - strand at 83.534 kb on + strand at 83.645 kb on + strand at 83.645 kb on + strand at 83.646 kb on - strand at 83.646 kb on - strand at 83.646 kb on - strand at 83.646 kb on - strand at 83.646 kb on - strand at 83.677 kb on + strand at 83.677 kb on + strand at 83.677 kb on + strand at 83.677 kb on + strand at 83.677 kb on + strand at 83.678 kb on - strand at 83.678 kb on - strand at 83.678 kb on - strand at 83.678 kb on - strand at 83.708 kb on + strand at 83.708 kb on + strand at 83.783 kb on - strand at 83.835 kb on + strand at 83.836 kb on - strand at 83.879 kb on + strand at 84.023 kb on + strand, within Pf1N1B4_5340 at 84.023 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.580 kb on + strand at 84.580 kb on + strand at 84.580 kb on + strand at 84.581 kb on - strand at 84.643 kb on - strand at 84.696 kb on + strand, within Pf1N1B4_5341 at 84.907 kb on + strand at 84.907 kb on + strand at 84.907 kb on + strand at 84.908 kb on - strand at 85.007 kb on + strand, within Pf1N1B4_5342 at 85.007 kb on + strand, within Pf1N1B4_5342 at 85.125 kb on - strand at 85.128 kb on + strand at 85.129 kb on + strand at 85.129 kb on + strand at 85.129 kb on + strand at 85.130 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A             remove   83,011   +    Pf1N1B4_5338   0.54  +0.2   83,011   +    Pf1N1B4_5338   0.54  -0.7   83,012   -    Pf1N1B4_5338   0.54  +0.2   83,012   -    Pf1N1B4_5338   0.54  +0.2   83,186   +        +0.2   83,397   +        -1.9   83,398   -        +0.2   83,513   +        -2.7   83,513   +        -1.3   83,514   -        +0.2   83,514   -        -4.1   83,514   -        +0.2   83,534   +        -2.0   83,645   +        +0.2   83,645   +        -0.3   83,646   -        -2.3   83,646   -        +0.2   83,646   -        -1.7   83,646   -        -3.0   83,646   -        -1.4   83,677   +        +0.2   83,677   +        +0.2   83,677   +        +0.2   83,677   +        +0.2   83,677   +        +0.2   83,678   -        +0.2   83,678   -        -2.1   83,678   -        +0.1   83,678   -        +0.2   83,708   +        +0.2   83,708   +        +0.2   83,783   -        +0.2   83,835   +        +0.2   83,836   -        -1.9   83,879   +        +0.2   84,023   +    Pf1N1B4_5340   0.20  +0.2   84,023   +    Pf1N1B4_5340   0.20  +0.2   84,187   +    Pf1N1B4_5340   0.66  -2.6   84,187   +    Pf1N1B4_5340   0.66  -2.3   84,187   +    Pf1N1B4_5340   0.66  -1.9   84,188   -    Pf1N1B4_5340   0.66  +0.2   84,188   -    Pf1N1B4_5340   0.66  +0.2   84,188   -    Pf1N1B4_5340   0.66  +0.2   84,580   +        +0.2   84,580   +        +0.2   84,580   +        +0.2   84,581   -        +0.2   84,643   -        +0.2   84,696   +    Pf1N1B4_5341   0.17  -2.2   84,907   +        -1.6   84,907   +        -4.0   84,907   +        -1.8   84,908   -        -0.4   85,007   +    Pf1N1B4_5342   0.39  -2.6   85,007   +    Pf1N1B4_5342   0.39  +0.2   85,125   -        +0.2   85,128   +        +0.2   85,129   +        -1.0   85,129   +        +0.2   85,129   +        -1.9   85,130   -        +0.2 
 
Or see this region's nucleotide sequence