Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5340

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5338 and Pf1N1B4_5339 are separated by 106 nucleotidesPf1N1B4_5339 and Pf1N1B4_5340 are separated by 608 nucleotidesPf1N1B4_5340 and Pf1N1B4_5341 are separated by 357 nucleotidesPf1N1B4_5341 and Pf1N1B4_5342 are separated by 109 nucleotidesPf1N1B4_5342 and Pf1N1B4_5343 are separated by 88 nucleotides Pf1N1B4_5338 - hypothetical protein, at 82,893 to 83,111 _5338 Pf1N1B4_5339 - hypothetical protein, at 83,218 to 83,340 _5339 Pf1N1B4_5340 - hypothetical protein, at 83,949 to 84,311 _5340 Pf1N1B4_5341 - hypothetical protein, at 84,669 to 84,830 _5341 Pf1N1B4_5342 - hypothetical protein, at 84,940 to 85,110 _5342 Pf1N1B4_5343 - hypothetical protein, at 85,199 to 85,933 _5343 Position (kb) 83 84 85Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 83.011 kb on + strand, within Pf1N1B4_5338at 83.011 kb on + strand, within Pf1N1B4_5338at 83.012 kb on - strand, within Pf1N1B4_5338at 83.012 kb on - strand, within Pf1N1B4_5338at 83.186 kb on + strandat 83.397 kb on + strandat 83.398 kb on - strandat 83.513 kb on + strandat 83.513 kb on + strandat 83.514 kb on - strandat 83.514 kb on - strandat 83.514 kb on - strandat 83.534 kb on + strandat 83.645 kb on + strandat 83.645 kb on + strandat 83.646 kb on - strandat 83.646 kb on - strandat 83.646 kb on - strandat 83.646 kb on - strandat 83.646 kb on - strandat 83.677 kb on + strandat 83.677 kb on + strandat 83.677 kb on + strandat 83.677 kb on + strandat 83.677 kb on + strandat 83.678 kb on - strandat 83.678 kb on - strandat 83.678 kb on - strandat 83.678 kb on - strandat 83.708 kb on + strandat 83.708 kb on + strandat 83.783 kb on - strandat 83.835 kb on + strandat 83.836 kb on - strandat 83.879 kb on + strandat 84.023 kb on + strand, within Pf1N1B4_5340at 84.023 kb on + strand, within Pf1N1B4_5340at 84.187 kb on + strand, within Pf1N1B4_5340at 84.187 kb on + strand, within Pf1N1B4_5340at 84.187 kb on + strand, within Pf1N1B4_5340at 84.188 kb on - strand, within Pf1N1B4_5340at 84.188 kb on - strand, within Pf1N1B4_5340at 84.188 kb on - strand, within Pf1N1B4_5340at 84.580 kb on + strandat 84.580 kb on + strandat 84.580 kb on + strandat 84.581 kb on - strandat 84.643 kb on - strandat 84.696 kb on + strand, within Pf1N1B4_5341at 84.907 kb on + strandat 84.907 kb on + strandat 84.907 kb on + strandat 84.908 kb on - strandat 85.007 kb on + strand, within Pf1N1B4_5342at 85.007 kb on + strand, within Pf1N1B4_5342at 85.125 kb on - strandat 85.128 kb on + strandat 85.129 kb on + strandat 85.129 kb on + strandat 85.129 kb on + strandat 85.130 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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83,011 + Pf1N1B4_5338 0.54 +0.2
83,011 + Pf1N1B4_5338 0.54 -0.7
83,012 - Pf1N1B4_5338 0.54 +0.2
83,012 - Pf1N1B4_5338 0.54 +0.2
83,186 + +0.2
83,397 + -1.9
83,398 - +0.2
83,513 + -2.7
83,513 + -1.3
83,514 - +0.2
83,514 - -4.1
83,514 - +0.2
83,534 + -2.0
83,645 + +0.2
83,645 + -0.3
83,646 - -2.3
83,646 - +0.2
83,646 - -1.7
83,646 - -3.0
83,646 - -1.4
83,677 + +0.2
83,677 + +0.2
83,677 + +0.2
83,677 + +0.2
83,677 + +0.2
83,678 - +0.2
83,678 - -2.1
83,678 - +0.1
83,678 - +0.2
83,708 + +0.2
83,708 + +0.2
83,783 - +0.2
83,835 + +0.2
83,836 - -1.9
83,879 + +0.2
84,023 + Pf1N1B4_5340 0.20 +0.2
84,023 + Pf1N1B4_5340 0.20 +0.2
84,187 + Pf1N1B4_5340 0.66 -2.6
84,187 + Pf1N1B4_5340 0.66 -2.3
84,187 + Pf1N1B4_5340 0.66 -1.9
84,188 - Pf1N1B4_5340 0.66 +0.2
84,188 - Pf1N1B4_5340 0.66 +0.2
84,188 - Pf1N1B4_5340 0.66 +0.2
84,580 + +0.2
84,580 + +0.2
84,580 + +0.2
84,581 - +0.2
84,643 - +0.2
84,696 + Pf1N1B4_5341 0.17 -2.2
84,907 + -1.6
84,907 + -4.0
84,907 + -1.8
84,908 - -0.4
85,007 + Pf1N1B4_5342 0.39 -2.6
85,007 + Pf1N1B4_5342 0.39 +0.2
85,125 - +0.2
85,128 + +0.2
85,129 + -1.0
85,129 + +0.2
85,129 + -1.9
85,130 - +0.2

Or see this region's nucleotide sequence