Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5269 and Pf1N1B4_5270 are separated by 604 nucleotides Pf1N1B4_5270 and Pf1N1B4_5271 are separated by 390 nucleotides Pf1N1B4_5271 and Pf1N1B4_5272 are separated by 255 nucleotides
Pf1N1B4_5269 - hypothetical protein, at 26,471 to 26,764
_5269
Pf1N1B4_5270 - hypothetical protein, at 27,369 to 28,193
_5270
Pf1N1B4_5271 - hypothetical protein, at 28,584 to 28,769
_5271
Pf1N1B4_5272 - hypothetical protein, at 29,025 to 29,201
_5272
Position (kb) 27
28
29 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 26.382 kb on - strand at 26.385 kb on + strand at 26.386 kb on - strand at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.522 kb on - strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.661 kb on - strand, within Pf1N1B4_5269 at 26.837 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 27.045 kb on + strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.145 kb on + strand at 27.146 kb on - strand at 27.181 kb on + strand at 27.182 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.238 kb on - strand at 27.269 kb on + strand at 27.294 kb on - strand at 27.332 kb on - strand at 27.365 kb on + strand at 27.366 kb on - strand at 27.433 kb on - strand at 27.493 kb on + strand, within Pf1N1B4_5270 at 27.500 kb on + strand at 27.594 kb on + strand, within Pf1N1B4_5270 at 27.595 kb on - strand at 27.613 kb on - strand, within Pf1N1B4_5270 at 27.637 kb on - strand, within Pf1N1B4_5270 at 27.672 kb on + strand, within Pf1N1B4_5270 at 27.693 kb on + strand at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.747 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270 at 27.831 kb on + strand, within Pf1N1B4_5270 at 27.832 kb on - strand, within Pf1N1B4_5270 at 27.858 kb on + strand, within Pf1N1B4_5270 at 27.858 kb on + strand, within Pf1N1B4_5270 at 27.858 kb on + strand, within Pf1N1B4_5270 at 27.859 kb on - strand, within Pf1N1B4_5270 at 27.859 kb on - strand, within Pf1N1B4_5270 at 27.859 kb on - strand, within Pf1N1B4_5270 at 27.859 kb on - strand, within Pf1N1B4_5270 at 27.859 kb on - strand, within Pf1N1B4_5270 at 27.875 kb on + strand, within Pf1N1B4_5270 at 27.909 kb on + strand, within Pf1N1B4_5270 at 27.909 kb on + strand, within Pf1N1B4_5270 at 27.910 kb on - strand, within Pf1N1B4_5270 at 27.910 kb on - strand, within Pf1N1B4_5270 at 27.953 kb on + strand, within Pf1N1B4_5270 at 27.953 kb on + strand, within Pf1N1B4_5270 at 27.953 kb on + strand, within Pf1N1B4_5270 at 27.954 kb on - strand, within Pf1N1B4_5270 at 27.954 kb on - strand, within Pf1N1B4_5270 at 27.954 kb on - strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.043 kb on + strand, within Pf1N1B4_5270 at 28.044 kb on - strand, within Pf1N1B4_5270 at 28.044 kb on - strand, within Pf1N1B4_5270 at 28.044 kb on - strand, within Pf1N1B4_5270 at 28.045 kb on + strand, within Pf1N1B4_5270 at 28.045 kb on + strand, within Pf1N1B4_5270 at 28.046 kb on - strand, within Pf1N1B4_5270 at 28.112 kb on + strand at 28.113 kb on - strand at 28.140 kb on + strand at 28.140 kb on + strand at 28.224 kb on + strand at 28.224 kb on + strand at 28.224 kb on + strand at 28.225 kb on - strand at 28.287 kb on + strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.288 kb on - strand at 28.350 kb on + strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.351 kb on - strand at 28.355 kb on + strand at 28.355 kb on + strand at 28.367 kb on + strand at 28.367 kb on + strand at 28.368 kb on - strand at 28.368 kb on - strand at 28.368 kb on - strand at 28.440 kb on + strand at 28.440 kb on + strand at 28.441 kb on - strand at 28.441 kb on - strand at 28.441 kb on - strand at 28.441 kb on - strand at 28.441 kb on - strand at 28.441 kb on - strand at 28.485 kb on + strand at 28.485 kb on + strand at 28.486 kb on - strand at 28.502 kb on - strand at 28.502 kb on - strand at 28.509 kb on + strand at 28.510 kb on - strand at 28.586 kb on - strand at 28.586 kb on - strand at 28.732 kb on - strand, within Pf1N1B4_5271 at 28.836 kb on + strand at 28.837 kb on - strand at 28.945 kb on - strand at 28.948 kb on - strand at 28.948 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 26,382 - -2.4 26,385 + -1.2 26,386 - -1.8 26,521 + Pf1N1B4_5269 0.17 +0.2 26,521 + Pf1N1B4_5269 0.17 +0.2 26,521 + Pf1N1B4_5269 0.17 +0.2 26,522 - Pf1N1B4_5269 0.17 +0.2 26,660 + Pf1N1B4_5269 0.64 +0.2 26,660 + Pf1N1B4_5269 0.64 +0.2 26,660 + Pf1N1B4_5269 0.64 -0.8 26,661 - Pf1N1B4_5269 0.65 -2.8 26,837 - +0.2 26,980 - +0.2 26,980 - -0.7 26,980 - -2.0 27,045 + +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,145 + +0.2 27,146 - -2.6 27,181 + -1.1 27,182 - +0.2 27,190 - +0.2 27,190 - +0.2 27,190 - -1.4 27,238 - -0.8 27,269 + +0.2 27,294 - -2.1 27,332 - +0.2 27,365 + +0.2 27,366 - +0.2 27,433 - +0.2 27,493 + Pf1N1B4_5270 0.15 +0.2 27,500 + -1.8 27,594 + Pf1N1B4_5270 0.27 -1.2 27,595 - -1.8 27,613 - Pf1N1B4_5270 0.30 +0.2 27,637 - Pf1N1B4_5270 0.32 +0.2 27,672 + Pf1N1B4_5270 0.37 -2.1 27,693 + -0.9 27,694 - Pf1N1B4_5270 0.39 +0.2 27,694 - Pf1N1B4_5270 0.39 +0.2 27,746 + Pf1N1B4_5270 0.46 +1.2 27,746 + Pf1N1B4_5270 0.46 -2.5 27,747 - Pf1N1B4_5270 0.46 +0.2 27,759 - Pf1N1B4_5270 0.47 +0.2 27,759 - Pf1N1B4_5270 0.47 -3.1 27,831 + Pf1N1B4_5270 0.56 +0.2 27,832 - Pf1N1B4_5270 0.56 +0.2 27,858 + Pf1N1B4_5270 0.59 +0.2 27,858 + Pf1N1B4_5270 0.59 +0.2 27,858 + Pf1N1B4_5270 0.59 -1.1 27,859 - Pf1N1B4_5270 0.59 +0.2 27,859 - Pf1N1B4_5270 0.59 +0.2 27,859 - Pf1N1B4_5270 0.59 +0.2 27,859 - Pf1N1B4_5270 0.59 +0.2 27,859 - Pf1N1B4_5270 0.59 +0.2 27,875 + Pf1N1B4_5270 0.61 +0.2 27,909 + Pf1N1B4_5270 0.65 -1.7 27,909 + Pf1N1B4_5270 0.65 +0.2 27,910 - Pf1N1B4_5270 0.66 +0.2 27,910 - Pf1N1B4_5270 0.66 +0.2 27,953 + Pf1N1B4_5270 0.71 -1.7 27,953 + Pf1N1B4_5270 0.71 -0.8 27,953 + Pf1N1B4_5270 0.71 +0.2 27,954 - Pf1N1B4_5270 0.71 +0.2 27,954 - Pf1N1B4_5270 0.71 +0.2 27,954 - Pf1N1B4_5270 0.71 +0.2 28,043 + Pf1N1B4_5270 0.82 +0.2 28,043 + Pf1N1B4_5270 0.82 +0.2 28,043 + Pf1N1B4_5270 0.82 -2.0 28,043 + Pf1N1B4_5270 0.82 +0.2 28,043 + Pf1N1B4_5270 0.82 -1.3 28,043 + Pf1N1B4_5270 0.82 +0.2 28,043 + Pf1N1B4_5270 0.82 -2.1 28,043 + Pf1N1B4_5270 0.82 -1.1 28,044 - Pf1N1B4_5270 0.82 -1.9 28,044 - Pf1N1B4_5270 0.82 +0.2 28,044 - Pf1N1B4_5270 0.82 +0.2 28,045 + Pf1N1B4_5270 0.82 +0.2 28,045 + Pf1N1B4_5270 0.82 -1.4 28,046 - Pf1N1B4_5270 0.82 +0.2 28,112 + +0.2 28,113 - +0.2 28,140 + +0.2 28,140 + +0.2 28,224 + -2.4 28,224 + +0.2 28,224 + +0.2 28,225 - +0.2 28,287 + -5.1 28,288 - -1.5 28,288 - -0.4 28,288 - -1.8 28,288 - -0.4 28,288 - +0.2 28,288 - +0.2 28,288 - +0.2 28,288 - +0.2 28,288 - +1.2 28,350 + +0.2 28,351 - +0.2 28,351 - +0.2 28,351 - +0.2 28,351 - -1.8 28,351 - -0.7 28,351 - +0.2 28,351 - -0.3 28,355 + -1.7 28,355 + +0.2 28,367 + -2.0 28,367 + +0.2 28,368 - +0.2 28,368 - +0.2 28,368 - +0.2 28,440 + +0.2 28,440 + +0.2 28,441 - +0.2 28,441 - +0.2 28,441 - -1.8 28,441 - -2.5 28,441 - -1.5 28,441 - +0.2 28,485 + +0.2 28,485 + +0.2 28,486 - +0.2 28,502 - +0.2 28,502 - +1.2 28,509 + -1.7 28,510 - +0.2 28,586 - +0.2 28,586 - -1.6 28,732 - Pf1N1B4_5271 0.80 -1.7 28,836 + -2.0 28,837 - +0.2 28,945 - +0.2 28,948 - +0.2 28,948 - +0.2 28,971 - +0.7 28,971 - -0.9 28,971 - +0.2 28,989 + -1.0
Or see this region's nucleotide sequence