Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5268 and Pf1N1B4_5269 are separated by 330 nucleotides Pf1N1B4_5269 and Pf1N1B4_5270 are separated by 604 nucleotides
Pf1N1B4_5268 - hypothetical protein, at 25,619 to 26,140
_5268
Pf1N1B4_5269 - hypothetical protein, at 26,471 to 26,764
_5269
Pf1N1B4_5270 - hypothetical protein, at 27,369 to 28,193
_5270
Position (kb) 26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.476 kb on + strand at 25.575 kb on + strand at 25.841 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.989 kb on + strand, within Pf1N1B4_5268 at 25.990 kb on - strand, within Pf1N1B4_5268 at 25.990 kb on - strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.055 kb on - strand, within Pf1N1B4_5268 at 26.058 kb on + strand at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.059 kb on - strand, within Pf1N1B4_5268 at 26.059 kb on - strand, within Pf1N1B4_5268 at 26.128 kb on - strand at 26.128 kb on - strand at 26.166 kb on + strand at 26.166 kb on + strand at 26.167 kb on - strand at 26.189 kb on + strand at 26.189 kb on + strand at 26.190 kb on - strand at 26.190 kb on - strand at 26.227 kb on - strand at 26.348 kb on + strand at 26.348 kb on + strand at 26.382 kb on - strand at 26.385 kb on + strand at 26.386 kb on - strand at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.522 kb on - strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.661 kb on - strand, within Pf1N1B4_5269 at 26.837 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 27.045 kb on + strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.145 kb on + strand at 27.146 kb on - strand at 27.181 kb on + strand at 27.182 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.238 kb on - strand at 27.269 kb on + strand at 27.294 kb on - strand at 27.332 kb on - strand at 27.365 kb on + strand at 27.366 kb on - strand at 27.433 kb on - strand at 27.493 kb on + strand, within Pf1N1B4_5270 at 27.500 kb on + strand at 27.594 kb on + strand, within Pf1N1B4_5270 at 27.595 kb on - strand at 27.613 kb on - strand, within Pf1N1B4_5270 at 27.637 kb on - strand, within Pf1N1B4_5270 at 27.672 kb on + strand, within Pf1N1B4_5270 at 27.693 kb on + strand at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.747 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 25,476 + +0.2 25,575 + +0.2 25,841 + Pf1N1B4_5268 0.43 -2.5 25,850 + Pf1N1B4_5268 0.44 +0.2 25,850 + Pf1N1B4_5268 0.44 -2.8 25,850 + Pf1N1B4_5268 0.44 +0.2 25,850 + Pf1N1B4_5268 0.44 -2.3 25,979 + Pf1N1B4_5268 0.69 +0.2 25,979 + Pf1N1B4_5268 0.69 +0.2 25,979 + Pf1N1B4_5268 0.69 +0.2 25,989 + Pf1N1B4_5268 0.71 +0.2 25,990 - Pf1N1B4_5268 0.71 -3.2 25,990 - Pf1N1B4_5268 0.71 +0.2 26,054 + Pf1N1B4_5268 0.83 +0.2 26,054 + Pf1N1B4_5268 0.83 +0.2 26,054 + Pf1N1B4_5268 0.83 +0.2 26,055 - Pf1N1B4_5268 0.84 +0.2 26,058 + +0.2 26,058 + Pf1N1B4_5268 0.84 +0.2 26,058 + Pf1N1B4_5268 0.84 +0.2 26,058 + Pf1N1B4_5268 0.84 -2.4 26,058 + Pf1N1B4_5268 0.84 -1.5 26,058 + Pf1N1B4_5268 0.84 -2.4 26,059 - Pf1N1B4_5268 0.84 +0.2 26,059 - Pf1N1B4_5268 0.84 +0.2 26,128 - +0.2 26,128 - -1.8 26,166 + -2.9 26,166 + -1.8 26,167 - -2.0 26,189 + -2.2 26,189 + +0.2 26,190 - -0.8 26,190 - +0.2 26,227 - +0.2 26,348 + +0.2 26,348 + +0.2 26,382 - -2.4 26,385 + -1.2 26,386 - -1.8 26,521 + Pf1N1B4_5269 0.17 +0.2 26,521 + Pf1N1B4_5269 0.17 +0.2 26,521 + Pf1N1B4_5269 0.17 +0.2 26,522 - Pf1N1B4_5269 0.17 +0.2 26,660 + Pf1N1B4_5269 0.64 +0.2 26,660 + Pf1N1B4_5269 0.64 +0.2 26,660 + Pf1N1B4_5269 0.64 -0.8 26,661 - Pf1N1B4_5269 0.65 -2.8 26,837 - +0.2 26,980 - +0.2 26,980 - -0.7 26,980 - -2.0 27,045 + +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,046 - +0.2 27,145 + +0.2 27,146 - -2.6 27,181 + -1.1 27,182 - +0.2 27,190 - +0.2 27,190 - +0.2 27,190 - -1.4 27,238 - -0.8 27,269 + +0.2 27,294 - -2.1 27,332 - +0.2 27,365 + +0.2 27,366 - +0.2 27,433 - +0.2 27,493 + Pf1N1B4_5270 0.15 +0.2 27,500 + -1.8 27,594 + Pf1N1B4_5270 0.27 -1.2 27,595 - -1.8 27,613 - Pf1N1B4_5270 0.30 +0.2 27,637 - Pf1N1B4_5270 0.32 +0.2 27,672 + Pf1N1B4_5270 0.37 -2.1 27,693 + -0.9 27,694 - Pf1N1B4_5270 0.39 +0.2 27,694 - Pf1N1B4_5270 0.39 +0.2 27,746 + Pf1N1B4_5270 0.46 +1.2 27,746 + Pf1N1B4_5270 0.46 -2.5 27,747 - Pf1N1B4_5270 0.46 +0.2 27,759 - Pf1N1B4_5270 0.47 +0.2 27,759 - Pf1N1B4_5270 0.47 -3.1
Or see this region's nucleotide sequence