Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5242

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5239 and Pf1N1B4_5240 are separated by 173 nucleotidesPf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotidesPf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotidesPf1N1B4_5242 and Pf1N1B4_5243 are separated by 71 nucleotidesPf1N1B4_5243 and Pf1N1B4_5244 are separated by 85 nucleotidesPf1N1B4_5244 and Pf1N1B4_5245 are separated by 42 nucleotides Pf1N1B4_5239 - hypothetical protein, at 184 to 579 _5239 Pf1N1B4_5240 - hypothetical protein, at 753 to 872 _5240 Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271 _5241 Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524 _5242 Pf1N1B4_5243 - hypothetical protein, at 1,596 to 1,763 _5243 Pf1N1B4_5244 - hypothetical protein, at 1,849 to 2,097 _5244 Pf1N1B4_5245 - hypothetical protein, at 2,140 to 2,784 _5245 Position (kb) 1 2Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 0.284 kb on + strand, within Pf1N1B4_5239at 0.285 kb on - strand, within Pf1N1B4_5239at 0.463 kb on - strand, within Pf1N1B4_5239at 0.494 kb on + strand, within Pf1N1B4_5239at 0.494 kb on + strandat 0.495 kb on - strand, within Pf1N1B4_5239at 0.496 kb on + strand, within Pf1N1B4_5239at 0.496 kb on + strand, within Pf1N1B4_5239at 0.496 kb on + strand, within Pf1N1B4_5239at 0.496 kb on + strand, within Pf1N1B4_5239at 0.497 kb on - strand, within Pf1N1B4_5239at 0.597 kb on - strandat 0.773 kb on - strand, within Pf1N1B4_5240at 0.773 kb on - strand, within Pf1N1B4_5240at 0.773 kb on - strand, within Pf1N1B4_5240at 0.943 kb on + strandat 0.943 kb on + strandat 0.992 kb on + strandat 1.074 kb on + strandat 1.074 kb on + strandat 1.074 kb on + strandat 1.074 kb on + strandat 1.074 kb on + strandat 1.075 kb on - strandat 1.075 kb on - strandat 1.092 kb on - strand, within Pf1N1B4_5241at 1.265 kb on + strandat 1.265 kb on + strandat 1.337 kb on - strand, within Pf1N1B4_5242at 1.369 kb on + strand, within Pf1N1B4_5242at 1.407 kb on + strand, within Pf1N1B4_5242at 1.407 kb on + strand, within Pf1N1B4_5242at 1.501 kb on + strandat 1.501 kb on + strandat 1.664 kb on + strand, within Pf1N1B4_5243at 1.664 kb on + strand, within Pf1N1B4_5243at 1.665 kb on - strand, within Pf1N1B4_5243at 1.689 kb on + strandat 1.786 kb on + strandat 1.787 kb on - strandat 1.953 kb on + strand, within Pf1N1B4_5244at 1.954 kb on - strand, within Pf1N1B4_5244at 2.026 kb on + strand, within Pf1N1B4_5244at 2.042 kb on + strand, within Pf1N1B4_5244at 2.096 kb on - strandat 2.096 kb on - strandat 2.239 kb on - strand, within Pf1N1B4_5245at 2.240 kb on + strandat 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.241 kb on - strand, within Pf1N1B4_5245at 2.265 kb on - strand, within Pf1N1B4_5245at 2.265 kb on - strand, within Pf1N1B4_5245at 2.324 kb on - strand, within Pf1N1B4_5245at 2.346 kb on - strand, within Pf1N1B4_5245at 2.370 kb on + strand, within Pf1N1B4_5245

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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284 + Pf1N1B4_5239 0.25 -2.7
285 - Pf1N1B4_5239 0.26 +0.2
463 - Pf1N1B4_5239 0.70 -2.1
494 + Pf1N1B4_5239 0.78 +0.2
494 + -1.0
495 - Pf1N1B4_5239 0.79 +0.2
496 + Pf1N1B4_5239 0.79 +0.2
496 + Pf1N1B4_5239 0.79 +0.2
496 + Pf1N1B4_5239 0.79 +0.2
496 + Pf1N1B4_5239 0.79 -1.5
497 - Pf1N1B4_5239 0.79 +0.2
597 - -2.7
773 - Pf1N1B4_5240 0.17 -0.2
773 - Pf1N1B4_5240 0.17 +0.5
773 - Pf1N1B4_5240 0.17 +0.2
943 + -1.4
943 + +0.2
992 + +0.8
1,074 + +0.2
1,074 + -0.8
1,074 + +0.2
1,074 + +0.2
1,074 + -1.6
1,075 - +0.2
1,075 - -1.7
1,092 - Pf1N1B4_5241 0.10 -1.2
1,265 + +0.2
1,265 + -1.0
1,337 - Pf1N1B4_5242 0.28 +0.2
1,369 + Pf1N1B4_5242 0.40 -3.8
1,407 + Pf1N1B4_5242 0.55 -1.3
1,407 + Pf1N1B4_5242 0.55 +0.2
1,501 + -1.3
1,501 + -2.5
1,664 + Pf1N1B4_5243 0.40 +0.2
1,664 + Pf1N1B4_5243 0.40 -1.7
1,665 - Pf1N1B4_5243 0.41 -2.9
1,689 + -2.7
1,786 + +0.2
1,787 - -0.0
1,953 + Pf1N1B4_5244 0.42 -2.1
1,954 - Pf1N1B4_5244 0.42 -3.1
2,026 + Pf1N1B4_5244 0.71 -0.8
2,042 + Pf1N1B4_5244 0.78 -1.1
2,096 - -1.6
2,096 - +0.2
2,239 - Pf1N1B4_5245 0.15 -1.6
2,240 + +0.2
2,241 - Pf1N1B4_5245 0.16 +0.2
2,241 - Pf1N1B4_5245 0.16 +0.2
2,241 - Pf1N1B4_5245 0.16 -1.8
2,241 - Pf1N1B4_5245 0.16 +0.2
2,241 - Pf1N1B4_5245 0.16 -1.5
2,241 - Pf1N1B4_5245 0.16 +0.3
2,241 - Pf1N1B4_5245 0.16 +0.2
2,265 - Pf1N1B4_5245 0.19 +0.2
2,265 - Pf1N1B4_5245 0.19 +0.2
2,324 - Pf1N1B4_5245 0.29 +0.2
2,346 - Pf1N1B4_5245 0.32 -0.5
2,370 + Pf1N1B4_5245 0.36 +0.2

Or see this region's nucleotide sequence