Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5149 and Pf1N1B4_5150 are separated by 2 nucleotides
Pf1N1B4_5149 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 5,732,960 to 5,733,703
_5149
Pf1N1B4_5150 - FIG00933687: hypothetical protein, at 5,733,706 to 5,736,129
_5150
Position (kb) 5733
5734
5735
5736
5737 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5732.860 kb on + strand at 5732.886 kb on - strand at 5733.172 kb on + strand, within Pf1N1B4_5149 at 5733.172 kb on + strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.258 kb on - strand, within Pf1N1B4_5149 at 5733.258 kb on - strand, within Pf1N1B4_5149 at 5733.497 kb on + strand, within Pf1N1B4_5149 at 5733.498 kb on - strand, within Pf1N1B4_5149 at 5733.579 kb on + strand, within Pf1N1B4_5149 at 5734.036 kb on + strand, within Pf1N1B4_5150 at 5734.036 kb on + strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.124 kb on - strand, within Pf1N1B4_5150 at 5734.360 kb on + strand at 5734.694 kb on - strand, within Pf1N1B4_5150 at 5734.930 kb on + strand, within Pf1N1B4_5150 at 5734.930 kb on + strand, within Pf1N1B4_5150 at 5734.930 kb on + strand at 5734.931 kb on - strand, within Pf1N1B4_5150 at 5734.931 kb on - strand, within Pf1N1B4_5150 at 5735.371 kb on + strand, within Pf1N1B4_5150 at 5735.590 kb on + strand at 5735.675 kb on - strand, within Pf1N1B4_5150 at 5735.827 kb on + strand, within Pf1N1B4_5150 at 5735.828 kb on - strand, within Pf1N1B4_5150 at 5736.101 kb on - strand at 5736.101 kb on - strand at 5736.182 kb on - strand at 5736.338 kb on + strand at 5736.339 kb on - strand at 5736.433 kb on + strand at 5736.481 kb on - strand at 5736.524 kb on - strand at 5736.546 kb on + strand at 5736.569 kb on - strand at 5736.921 kb on + strand at 5736.921 kb on + strand at 5736.999 kb on - strand at 5737.003 kb on - strand at 5737.009 kb on - strand at 5737.078 kb on + strand at 5737.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,732,860 + -2.8 5,732,886 - -0.0 5,733,172 + Pf1N1B4_5149 0.28 -0.0 5,733,172 + Pf1N1B4_5149 0.28 -0.0 5,733,173 - Pf1N1B4_5149 0.29 -0.0 5,733,173 - Pf1N1B4_5149 0.29 -0.0 5,733,173 - Pf1N1B4_5149 0.29 -0.0 5,733,173 - -2.8 5,733,173 - Pf1N1B4_5149 0.29 -1.4 5,733,257 + Pf1N1B4_5149 0.40 -2.6 5,733,257 + Pf1N1B4_5149 0.40 -1.3 5,733,257 + Pf1N1B4_5149 0.40 -0.0 5,733,258 - Pf1N1B4_5149 0.40 -2.1 5,733,258 - Pf1N1B4_5149 0.40 -1.7 5,733,497 + Pf1N1B4_5149 0.72 -1.7 5,733,498 - Pf1N1B4_5149 0.72 -0.0 5,733,579 + Pf1N1B4_5149 0.83 -0.0 5,734,036 + Pf1N1B4_5150 0.14 -2.9 5,734,036 + Pf1N1B4_5150 0.14 -0.0 5,734,037 - Pf1N1B4_5150 0.14 -1.4 5,734,037 - Pf1N1B4_5150 0.14 -0.0 5,734,037 - Pf1N1B4_5150 0.14 -2.6 5,734,124 - Pf1N1B4_5150 0.17 -0.0 5,734,360 + -0.0 5,734,694 - Pf1N1B4_5150 0.41 -0.0 5,734,930 + Pf1N1B4_5150 0.50 -1.3 5,734,930 + Pf1N1B4_5150 0.50 -0.0 5,734,930 + -0.0 5,734,931 - Pf1N1B4_5150 0.51 -2.2 5,734,931 - Pf1N1B4_5150 0.51 -0.0 5,735,371 + Pf1N1B4_5150 0.69 -1.1 5,735,590 + -0.0 5,735,675 - Pf1N1B4_5150 0.81 -0.0 5,735,827 + Pf1N1B4_5150 0.88 -0.0 5,735,828 - Pf1N1B4_5150 0.88 -0.0 5,736,101 - -1.4 5,736,101 - -0.0 5,736,182 - -2.6 5,736,338 + -1.3 5,736,339 - -0.0 5,736,433 + -0.0 5,736,481 - -0.0 5,736,524 - -0.0 5,736,546 + -0.0 5,736,569 - +1.0 5,736,921 + -0.0 5,736,921 + -0.0 5,736,999 - -0.0 5,737,003 - +0.6 5,737,009 - -0.0 5,737,078 + -0.0 5,737,127 + -0.0
Or see this region's nucleotide sequence