Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5112 and Pf1N1B4_5113 are separated by 96 nucleotides Pf1N1B4_5113 and Pf1N1B4_5114 overlap by 4 nucleotides Pf1N1B4_5114 and Pf1N1B4_5115 are separated by 27 nucleotides
Pf1N1B4_5112 - sucrose ABC transporter, substrate-binding component <small>(from data)</small>, at 5,671,718 to 5,672,890
_5112
Pf1N1B4_5113 - sucrose ABC transporter, permease component 1 <small>(from data)</small>, at 5,672,987 to 5,673,874
_5113
Pf1N1B4_5114 - sucrose ABC transporter, permease component 2 <small>(from data)</small>, at 5,673,871 to 5,674,713
_5114
Pf1N1B4_5115 - sucrose ABC transporter, ATPase component <small>(from data)</small>, at 5,674,741 to 5,675,886
_5115
Position (kb) 5672
5673
5674 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5671.993 kb on - strand, within Pf1N1B4_5112 at 5672.043 kb on - strand, within Pf1N1B4_5112 at 5672.069 kb on + strand, within Pf1N1B4_5112 at 5672.070 kb on - strand, within Pf1N1B4_5112 at 5672.168 kb on + strand at 5672.169 kb on - strand, within Pf1N1B4_5112 at 5672.411 kb on - strand, within Pf1N1B4_5112 at 5672.639 kb on + strand at 5672.640 kb on - strand, within Pf1N1B4_5112 at 5672.973 kb on + strand at 5673.134 kb on + strand, within Pf1N1B4_5113 at 5673.134 kb on + strand, within Pf1N1B4_5113 at 5673.213 kb on - strand at 5673.246 kb on + strand, within Pf1N1B4_5113 at 5673.692 kb on + strand, within Pf1N1B4_5113 at 5673.692 kb on + strand, within Pf1N1B4_5113 at 5673.693 kb on - strand, within Pf1N1B4_5113 at 5673.737 kb on + strand, within Pf1N1B4_5113 at 5673.737 kb on + strand, within Pf1N1B4_5113 at 5674.045 kb on + strand, within Pf1N1B4_5114 at 5674.476 kb on - strand, within Pf1N1B4_5114 at 5674.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,671,993 - Pf1N1B4_5112 0.23 -0.0 5,672,043 - Pf1N1B4_5112 0.28 -0.0 5,672,069 + Pf1N1B4_5112 0.30 -0.0 5,672,070 - Pf1N1B4_5112 0.30 -3.5 5,672,168 + -0.0 5,672,169 - Pf1N1B4_5112 0.38 -1.0 5,672,411 - Pf1N1B4_5112 0.59 -2.0 5,672,639 + -2.6 5,672,640 - Pf1N1B4_5112 0.79 -1.6 5,672,973 + -2.5 5,673,134 + Pf1N1B4_5113 0.17 -2.8 5,673,134 + Pf1N1B4_5113 0.17 -1.6 5,673,213 - -0.0 5,673,246 + Pf1N1B4_5113 0.29 -0.0 5,673,692 + Pf1N1B4_5113 0.79 -2.1 5,673,692 + Pf1N1B4_5113 0.79 -0.0 5,673,693 - Pf1N1B4_5113 0.80 -0.0 5,673,737 + Pf1N1B4_5113 0.84 -2.1 5,673,737 + Pf1N1B4_5113 0.84 -0.0 5,674,045 + Pf1N1B4_5114 0.21 -2.7 5,674,476 - Pf1N1B4_5114 0.72 -0.0 5,674,792 - -1.0
Or see this region's nucleotide sequence