Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4934 and Pf1N1B4_4935 overlap by 4 nucleotides Pf1N1B4_4935 and Pf1N1B4_4936 are separated by 128 nucleotides
Pf1N1B4_4934 - DNA damage response nuclease <small>(from data)</small>, at 5,468,400 to 5,470,052
_4934
Pf1N1B4_4935 - DinG family ATP-dependent helicase CPE1197, at 5,470,049 to 5,472,328
_4935
Pf1N1B4_4936 - hypothetical protein, at 5,472,457 to 5,473,362
_4936
Position (kb) 5470
5471
5472
5473 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5469.349 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand at 5469.520 kb on - strand, within Pf1N1B4_4934 at 5469.705 kb on + strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.730 kb on - strand at 5469.764 kb on - strand at 5470.055 kb on + strand at 5470.295 kb on + strand, within Pf1N1B4_4935 at 5470.296 kb on - strand at 5470.361 kb on + strand, within Pf1N1B4_4935 at 5470.479 kb on + strand at 5470.479 kb on + strand, within Pf1N1B4_4935 at 5470.929 kb on - strand at 5471.031 kb on - strand at 5471.031 kb on - strand, within Pf1N1B4_4935 at 5471.067 kb on + strand, within Pf1N1B4_4935 at 5471.067 kb on + strand at 5471.079 kb on - strand, within Pf1N1B4_4935 at 5471.079 kb on - strand at 5471.084 kb on + strand, within Pf1N1B4_4935 at 5471.085 kb on - strand, within Pf1N1B4_4935 at 5471.088 kb on - strand, within Pf1N1B4_4935 at 5471.132 kb on - strand, within Pf1N1B4_4935 at 5471.195 kb on + strand, within Pf1N1B4_4935 at 5471.196 kb on - strand at 5471.196 kb on - strand, within Pf1N1B4_4935 at 5471.196 kb on - strand, within Pf1N1B4_4935 at 5471.548 kb on - strand, within Pf1N1B4_4935 at 5471.756 kb on + strand, within Pf1N1B4_4935 at 5471.831 kb on + strand, within Pf1N1B4_4935 at 5471.832 kb on - strand, within Pf1N1B4_4935 at 5471.977 kb on + strand, within Pf1N1B4_4935 at 5471.977 kb on + strand, within Pf1N1B4_4935 at 5472.149 kb on + strand at 5472.150 kb on - strand at 5472.150 kb on - strand at 5472.457 kb on - strand at 5472.475 kb on + strand at 5472.597 kb on + strand at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.662 kb on - strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.187 kb on - strand at 5473.187 kb on - strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.189 kb on - strand, within Pf1N1B4_4936 at 5473.225 kb on + strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.292 kb on - strand at 5473.318 kb on + strand at 5473.318 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,469,349 + Pf1N1B4_4934 0.57 -2.1 5,469,519 + Pf1N1B4_4934 0.68 -1.0 5,469,519 + -0.0 5,469,520 - Pf1N1B4_4934 0.68 -2.3 5,469,705 + Pf1N1B4_4934 0.79 -1.5 5,469,706 - Pf1N1B4_4934 0.79 -0.0 5,469,706 - Pf1N1B4_4934 0.79 -0.0 5,469,730 - -1.9 5,469,764 - -0.0 5,470,055 + -1.6 5,470,295 + Pf1N1B4_4935 0.11 -0.0 5,470,296 - -1.2 5,470,361 + Pf1N1B4_4935 0.14 -2.7 5,470,479 + -1.2 5,470,479 + Pf1N1B4_4935 0.19 -1.4 5,470,929 - -0.0 5,471,031 - -0.0 5,471,031 - Pf1N1B4_4935 0.43 -4.2 5,471,067 + Pf1N1B4_4935 0.45 -2.2 5,471,067 + -1.9 5,471,079 - Pf1N1B4_4935 0.45 -0.7 5,471,079 - -0.0 5,471,084 + Pf1N1B4_4935 0.45 -0.0 5,471,085 - Pf1N1B4_4935 0.45 -0.0 5,471,088 - Pf1N1B4_4935 0.46 -1.9 5,471,132 - Pf1N1B4_4935 0.47 -1.4 5,471,195 + Pf1N1B4_4935 0.50 -0.0 5,471,196 - -0.0 5,471,196 - Pf1N1B4_4935 0.50 -0.0 5,471,196 - Pf1N1B4_4935 0.50 -2.1 5,471,548 - Pf1N1B4_4935 0.66 -2.2 5,471,756 + Pf1N1B4_4935 0.75 -3.3 5,471,831 + Pf1N1B4_4935 0.78 -5.1 5,471,832 - Pf1N1B4_4935 0.78 -3.9 5,471,977 + Pf1N1B4_4935 0.85 -0.0 5,471,977 + Pf1N1B4_4935 0.85 -0.0 5,472,149 + +0.2 5,472,150 - -1.7 5,472,150 - -2.2 5,472,457 - -2.5 5,472,475 + +0.6 5,472,597 + -1.2 5,472,649 + Pf1N1B4_4936 0.21 -2.2 5,472,649 + Pf1N1B4_4936 0.21 -0.0 5,472,662 - Pf1N1B4_4936 0.23 -1.9 5,473,135 + Pf1N1B4_4936 0.75 -2.5 5,473,135 + Pf1N1B4_4936 0.75 -0.0 5,473,187 - -3.1 5,473,187 - Pf1N1B4_4936 0.81 -0.0 5,473,188 + Pf1N1B4_4936 0.81 -0.0 5,473,188 + Pf1N1B4_4936 0.81 -0.0 5,473,188 + Pf1N1B4_4936 0.81 -1.0 5,473,189 - Pf1N1B4_4936 0.81 -0.0 5,473,225 + Pf1N1B4_4936 0.85 -2.1 5,473,226 - Pf1N1B4_4936 0.85 -0.0 5,473,226 - Pf1N1B4_4936 0.85 -3.0 5,473,292 - -1.0 5,473,318 + -0.0 5,473,318 + -0.0
Or see this region's nucleotide sequence