Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4928 and Pf1N1B4_4929 overlap by 4 nucleotides Pf1N1B4_4929 and Pf1N1B4_4930 overlap by 4 nucleotides
Pf1N1B4_4928 - Ferredoxin reductase, at 5,461,238 to 5,462,629
_4928
Pf1N1B4_4929 - Opine oxidase subunit B, at 5,462,626 to 5,463,795
_4929
Pf1N1B4_4930 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,463,792 to 5,464,949
_4930
Position (kb) 5462
5463
5464 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.650 kb on - strand, within Pf1N1B4_4928 at 5461.650 kb on - strand at 5461.739 kb on + strand, within Pf1N1B4_4928 at 5461.740 kb on - strand, within Pf1N1B4_4928 at 5461.757 kb on + strand, within Pf1N1B4_4928 at 5461.935 kb on + strand, within Pf1N1B4_4928 at 5462.077 kb on + strand, within Pf1N1B4_4928 at 5462.154 kb on - strand at 5462.154 kb on - strand, within Pf1N1B4_4928 at 5462.634 kb on - strand at 5463.325 kb on + strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.460 kb on + strand at 5463.566 kb on - strand, within Pf1N1B4_4929 at 5463.619 kb on + strand, within Pf1N1B4_4929 at 5463.620 kb on - strand, within Pf1N1B4_4929 at 5463.623 kb on - strand, within Pf1N1B4_4929 at 5463.877 kb on - strand at 5463.877 kb on - strand at 5463.877 kb on - strand at 5464.012 kb on + strand, within Pf1N1B4_4930 at 5464.012 kb on + strand, within Pf1N1B4_4930 at 5464.013 kb on - strand, within Pf1N1B4_4930 at 5464.327 kb on - strand, within Pf1N1B4_4930
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,461,649 + Pf1N1B4_4928 0.30 -0.0 5,461,649 + Pf1N1B4_4928 0.30 -0.0 5,461,649 + Pf1N1B4_4928 0.30 -0.0 5,461,649 + Pf1N1B4_4928 0.30 -1.0 5,461,650 - Pf1N1B4_4928 0.30 -0.3 5,461,650 - -1.7 5,461,739 + Pf1N1B4_4928 0.36 -0.0 5,461,740 - Pf1N1B4_4928 0.36 -1.7 5,461,757 + Pf1N1B4_4928 0.37 -1.9 5,461,935 + Pf1N1B4_4928 0.50 -0.0 5,462,077 + Pf1N1B4_4928 0.60 -1.2 5,462,154 - -1.5 5,462,154 - Pf1N1B4_4928 0.66 -0.0 5,462,634 - -0.3 5,463,325 + Pf1N1B4_4929 0.60 -0.0 5,463,353 - Pf1N1B4_4929 0.62 -0.0 5,463,353 - Pf1N1B4_4929 0.62 -0.0 5,463,379 + Pf1N1B4_4929 0.64 -0.0 5,463,379 + Pf1N1B4_4929 0.64 -0.0 5,463,460 + -0.9 5,463,566 - Pf1N1B4_4929 0.80 -3.4 5,463,619 + Pf1N1B4_4929 0.85 -0.0 5,463,620 - Pf1N1B4_4929 0.85 -0.0 5,463,623 - Pf1N1B4_4929 0.85 -0.0 5,463,877 - -0.0 5,463,877 - -2.2 5,463,877 - -0.0 5,464,012 + Pf1N1B4_4930 0.19 -1.7 5,464,012 + Pf1N1B4_4930 0.19 -0.0 5,464,013 - Pf1N1B4_4930 0.19 -0.0 5,464,327 - Pf1N1B4_4930 0.46 -0.0
Or see this region's nucleotide sequence