Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4611 and Pf1N1B4_4612 are separated by 30 nucleotides Pf1N1B4_4612 and Pf1N1B4_4613 overlap by 4 nucleotides Pf1N1B4_4613 and Pf1N1B4_4614 are separated by 10 nucleotides
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Pf1N1B4_4612 - FIG00954117: hypothetical protein, at 5,081,804 to 5,082,064
_4612
Pf1N1B4_4613 - FIG00953934: hypothetical protein, at 5,082,061 to 5,082,891
_4613
Pf1N1B4_4614 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 5,082,902 to 5,085,559
_4614
Position (kb) 5082
5083 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5081.136 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.744 kb on + strand at 5081.744 kb on + strand at 5081.745 kb on - strand at 5081.772 kb on - strand at 5081.776 kb on + strand at 5081.776 kb on + strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.987 kb on + strand at 5081.988 kb on - strand, within Pf1N1B4_4612 at 5082.197 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.578 kb on + strand, within Pf1N1B4_4613 at 5082.855 kb on + strand at 5082.859 kb on + strand at 5082.859 kb on + strand at 5082.860 kb on - strand at 5082.902 kb on - strand at 5083.140 kb on - strand at 5083.247 kb on + strand, within Pf1N1B4_4614 at 5083.248 kb on - strand, within Pf1N1B4_4614 at 5083.308 kb on - strand at 5083.337 kb on - strand, within Pf1N1B4_4614 at 5083.345 kb on + strand, within Pf1N1B4_4614 at 5083.559 kb on + strand, within Pf1N1B4_4614 at 5083.560 kb on - strand at 5083.791 kb on + strand at 5083.792 kb on - strand, within Pf1N1B4_4614
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,081,136 + Pf1N1B4_4611 0.27 -0.0 5,081,477 + Pf1N1B4_4611 0.66 -0.0 5,081,477 + Pf1N1B4_4611 0.66 -2.9 5,081,477 + Pf1N1B4_4611 0.66 -0.0 5,081,477 + Pf1N1B4_4611 0.66 -0.0 5,081,477 + Pf1N1B4_4611 0.66 -1.9 5,081,744 + -0.0 5,081,744 + -2.0 5,081,745 - -0.0 5,081,772 - -0.0 5,081,776 + -2.4 5,081,776 + -1.6 5,081,777 - -0.0 5,081,777 - -0.0 5,081,777 - -0.4 5,081,849 + Pf1N1B4_4612 0.17 -0.0 5,081,849 + Pf1N1B4_4612 0.17 -1.0 5,081,850 - Pf1N1B4_4612 0.18 +0.4 5,081,850 - Pf1N1B4_4612 0.18 -0.0 5,081,850 - Pf1N1B4_4612 0.18 -0.0 5,081,987 + -0.0 5,081,988 - Pf1N1B4_4612 0.70 -1.6 5,082,197 - Pf1N1B4_4613 0.16 -1.9 5,082,329 - Pf1N1B4_4613 0.32 -0.0 5,082,329 - Pf1N1B4_4613 0.32 -2.4 5,082,578 + Pf1N1B4_4613 0.62 -2.1 5,082,855 + -0.0 5,082,859 + -0.0 5,082,859 + -0.0 5,082,860 - -1.3 5,082,902 - -2.4 5,083,140 - -0.0 5,083,247 + Pf1N1B4_4614 0.13 -0.0 5,083,248 - Pf1N1B4_4614 0.13 -0.0 5,083,308 - -0.0 5,083,337 - Pf1N1B4_4614 0.16 -0.0 5,083,345 + Pf1N1B4_4614 0.17 -0.0 5,083,559 + Pf1N1B4_4614 0.25 -2.2 5,083,560 - -2.4 5,083,791 + -0.4 5,083,792 - Pf1N1B4_4614 0.33 -1.7
Or see this region's nucleotide sequence