Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4427

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4425 and Pf1N1B4_4426 overlap by 4 nucleotidesPf1N1B4_4426 and Pf1N1B4_4427 overlap by 11 nucleotidesPf1N1B4_4427 and Pf1N1B4_4428 overlap by 14 nucleotidesPf1N1B4_4428 and Pf1N1B4_4429 are separated by 17 nucleotides Pf1N1B4_4425 - Aspartate aminotransferase (EC 2.6.1.1), at 4,885,764 to 4,886,975 _4425 Pf1N1B4_4426 - Glutamate transport membrane-spanning protein, at 4,886,972 to 4,887,706 _4426 Pf1N1B4_4427 - Glutamate transport membrane-spanning protein, at 4,887,696 to 4,888,484 _4427 Pf1N1B4_4428 - ABC-type polar amino acid transport system, ATPase component, at 4,888,471 to 4,889,307 _4428 Pf1N1B4_4429 - No significant database matches, at 4,889,325 to 4,889,921 _4429 Position (kb) 4887 4888 4889Strain fitness (log2 ratio) -3 -2 -1 0 1at 4886.744 kb on - strand, within Pf1N1B4_4425at 4886.748 kb on - strand, within Pf1N1B4_4425at 4887.026 kb on + strandat 4887.558 kb on - strand, within Pf1N1B4_4426at 4888.017 kb on + strand, within Pf1N1B4_4427at 4888.029 kb on + strand, within Pf1N1B4_4427at 4888.029 kb on + strand, within Pf1N1B4_4427at 4888.029 kb on + strand, within Pf1N1B4_4427at 4888.029 kb on + strand, within Pf1N1B4_4427at 4888.029 kb on + strand, within Pf1N1B4_4427at 4888.030 kb on - strand, within Pf1N1B4_4427at 4888.065 kb on + strand, within Pf1N1B4_4427at 4888.065 kb on + strand, within Pf1N1B4_4427at 4888.391 kb on + strand, within Pf1N1B4_4427at 4888.395 kb on + strand, within Pf1N1B4_4427at 4888.395 kb on + strand, within Pf1N1B4_4427at 4888.763 kb on - strand, within Pf1N1B4_4428at 4889.305 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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4,886,744 - Pf1N1B4_4425 0.81 -0.1
4,886,748 - Pf1N1B4_4425 0.81 -0.1
4,887,026 + -0.1
4,887,558 - Pf1N1B4_4426 0.80 -0.1
4,888,017 + Pf1N1B4_4427 0.41 -2.9
4,888,029 + Pf1N1B4_4427 0.42 -0.1
4,888,029 + Pf1N1B4_4427 0.42 -2.2
4,888,029 + Pf1N1B4_4427 0.42 -0.1
4,888,029 + Pf1N1B4_4427 0.42 -1.9
4,888,029 + Pf1N1B4_4427 0.42 -0.1
4,888,030 - Pf1N1B4_4427 0.42 -0.1
4,888,065 + Pf1N1B4_4427 0.47 -2.2
4,888,065 + Pf1N1B4_4427 0.47 -0.1
4,888,391 + Pf1N1B4_4427 0.88 -0.1
4,888,395 + Pf1N1B4_4427 0.89 -0.1
4,888,395 + Pf1N1B4_4427 0.89 -0.1
4,888,763 - Pf1N1B4_4428 0.35 -0.1
4,889,305 + -0.1

Or see this region's nucleotide sequence