Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4425 and Pf1N1B4_4426 overlap by 4 nucleotides Pf1N1B4_4426 and Pf1N1B4_4427 overlap by 11 nucleotides Pf1N1B4_4427 and Pf1N1B4_4428 overlap by 14 nucleotides Pf1N1B4_4428 and Pf1N1B4_4429 are separated by 17 nucleotides
Pf1N1B4_4425 - Aspartate aminotransferase (EC 2.6.1.1), at 4,885,764 to 4,886,975
_4425
Pf1N1B4_4426 - Glutamate transport membrane-spanning protein, at 4,886,972 to 4,887,706
_4426
Pf1N1B4_4427 - Glutamate transport membrane-spanning protein, at 4,887,696 to 4,888,484
_4427
Pf1N1B4_4428 - ABC-type polar amino acid transport system, ATPase component, at 4,888,471 to 4,889,307
_4428
Pf1N1B4_4429 - No significant database matches, at 4,889,325 to 4,889,921
_4429
Position (kb)
4887
4888
4889 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4886.744 kb on - strand, within Pf1N1B4_4425 at 4886.748 kb on - strand, within Pf1N1B4_4425 at 4887.026 kb on + strand at 4887.558 kb on - strand, within Pf1N1B4_4426 at 4888.017 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.030 kb on - strand, within Pf1N1B4_4427 at 4888.065 kb on + strand, within Pf1N1B4_4427 at 4888.065 kb on + strand, within Pf1N1B4_4427 at 4888.391 kb on + strand, within Pf1N1B4_4427 at 4888.395 kb on + strand, within Pf1N1B4_4427 at 4888.395 kb on + strand, within Pf1N1B4_4427 at 4888.763 kb on - strand, within Pf1N1B4_4428 at 4889.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,886,744 - Pf1N1B4_4425 0.81 -0.1 4,886,748 - Pf1N1B4_4425 0.81 -0.1 4,887,026 + -0.1 4,887,558 - Pf1N1B4_4426 0.80 -0.1 4,888,017 + Pf1N1B4_4427 0.41 -2.9 4,888,029 + Pf1N1B4_4427 0.42 -0.1 4,888,029 + Pf1N1B4_4427 0.42 -2.2 4,888,029 + Pf1N1B4_4427 0.42 -0.1 4,888,029 + Pf1N1B4_4427 0.42 -1.9 4,888,029 + Pf1N1B4_4427 0.42 -0.1 4,888,030 - Pf1N1B4_4427 0.42 -0.1 4,888,065 + Pf1N1B4_4427 0.47 -2.2 4,888,065 + Pf1N1B4_4427 0.47 -0.1 4,888,391 + Pf1N1B4_4427 0.88 -0.1 4,888,395 + Pf1N1B4_4427 0.89 -0.1 4,888,395 + Pf1N1B4_4427 0.89 -0.1 4,888,763 - Pf1N1B4_4428 0.35 -0.1 4,889,305 + -0.1
Or see this region's nucleotide sequence