Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4392

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
4,851,446	+		Pf1N1B4_4391	0.33	-0.1
4,851,446	+		Pf1N1B4_4391	0.33	-2.0
4,851,737	+		Pf1N1B4_4391	0.60	-2.9
4,851,737	+		Pf1N1B4_4391	0.60	-0.1
4,851,765	-				-1.1
4,851,905	+		Pf1N1B4_4391	0.75	-2.2
4,851,905	+		Pf1N1B4_4391	0.75	-1.2
4,851,905	+		Pf1N1B4_4391	0.75	-0.1
4,851,906	-		Pf1N1B4_4391	0.75	-1.0
4,851,906	-		Pf1N1B4_4391	0.75	-0.1
4,851,932	+		Pf1N1B4_4391	0.77	-4.5
4,851,947	+		Pf1N1B4_4391	0.78	-0.1
4,851,947	+				+1.5
4,851,948	-		Pf1N1B4_4391	0.79	-1.4
4,852,037	+				-0.1
4,852,037	+		Pf1N1B4_4391	0.87	-2.3
4,852,106	+				-1.5
4,852,194	+				-0.1
4,852,264	+				-0.1
4,852,264	+				-1.9
4,852,265	-				-0.1
4,852,315	-				-0.1
4,852,320	+				-0.1
4,852,321	-				-0.1
4,852,321	-				-0.8
4,852,321	-				-2.4
4,852,358	+				-0.1
4,852,358	+				-0.1
4,852,358	+				-0.1
4,852,358	+				-0.1
4,852,358	+				-0.1
4,852,359	-				-2.2
4,852,420	+		Pf1N1B4_4392	0.26	-0.1
4,852,420	+		Pf1N1B4_4392	0.26	-0.1
4,852,421	-		Pf1N1B4_4392	0.26	-0.1
4,852,421	-				-0.1
4,852,421	-		Pf1N1B4_4392	0.26	-0.1
4,852,606	+		Pf1N1B4_4392	0.84	-2.6
4,852,606	+				-0.8
4,852,606	+		Pf1N1B4_4392	0.84	-2.5
4,852,606	+		Pf1N1B4_4392	0.84	-2.9
4,852,607	-				-1.6
4,852,607	-		Pf1N1B4_4392	0.84	-1.1
4,852,607	-		Pf1N1B4_4392	0.84	-0.1
4,852,654	+				-0.1
4,852,655	-				-0.1
4,853,329	-		Pf1N1B4_4393	0.53	-0.1