Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4285 and Pf1N1B4_4286 are separated by 54 nucleotides Pf1N1B4_4286 and Pf1N1B4_4287 are separated by 57 nucleotides
Pf1N1B4_4285 - Inositol transport system sugar-binding protein, at 4,750,312 to 4,751,238
_4285
Pf1N1B4_4286 - Inositol transport system ATP-binding protein, at 4,751,293 to 4,752,873
_4286
Pf1N1B4_4287 - Inositol transport system permease protein, at 4,752,931 to 4,753,953
_4287
Position (kb)
4751
4752
4753 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4750.383 kb on + strand at 4750.432 kb on + strand, within Pf1N1B4_4285 at 4750.535 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.739 kb on - strand, within Pf1N1B4_4285 at 4750.849 kb on + strand, within Pf1N1B4_4285 at 4751.236 kb on + strand at 4751.237 kb on - strand at 4751.261 kb on + strand at 4751.261 kb on + strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.299 kb on + strand at 4751.383 kb on + strand at 4751.536 kb on + strand, within Pf1N1B4_4286 at 4751.849 kb on - strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.032 kb on - strand, within Pf1N1B4_4286 at 4752.032 kb on - strand at 4752.454 kb on + strand at 4752.497 kb on + strand, within Pf1N1B4_4286 at 4752.498 kb on - strand, within Pf1N1B4_4286 at 4752.680 kb on - strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.694 kb on + strand, within Pf1N1B4_4286 at 4752.694 kb on + strand, within Pf1N1B4_4286 at 4752.750 kb on - strand at 4752.914 kb on + strand at 4752.914 kb on + strand at 4752.914 kb on + strand at 4752.915 kb on - strand at 4753.005 kb on + strand at 4753.005 kb on + strand at 4753.005 kb on + strand at 4753.426 kb on + strand, within Pf1N1B4_4287 at 4753.427 kb on - strand, within Pf1N1B4_4287 at 4753.552 kb on + strand, within Pf1N1B4_4287 at 4753.553 kb on - strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.568 kb on - strand, within Pf1N1B4_4287 at 4753.639 kb on + strand, within Pf1N1B4_4287 at 4753.726 kb on + strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.846 kb on - strand, within Pf1N1B4_4287 at 4753.867 kb on + strand at 4753.867 kb on + strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.869 kb on + strand at 4753.869 kb on + strand at 4753.869 kb on + strand at 4753.870 kb on - strand at 4753.870 kb on - strand at 4753.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,750,383 + -0.1 4,750,432 + Pf1N1B4_4285 0.13 -0.6 4,750,535 + Pf1N1B4_4285 0.24 -4.1 4,750,645 + -0.1 4,750,645 + Pf1N1B4_4285 0.36 -4.4 4,750,645 + Pf1N1B4_4285 0.36 -0.1 4,750,739 - Pf1N1B4_4285 0.46 -1.1 4,750,849 + Pf1N1B4_4285 0.58 -3.1 4,751,236 + -0.1 4,751,237 - -0.1 4,751,261 + -0.1 4,751,261 + -0.1 4,751,262 - -0.1 4,751,262 - -0.1 4,751,262 - -0.1 4,751,299 + -0.1 4,751,383 + -0.1 4,751,536 + Pf1N1B4_4286 0.15 -0.1 4,751,849 - Pf1N1B4_4286 0.35 -1.5 4,751,962 + Pf1N1B4_4286 0.42 -0.1 4,751,962 + Pf1N1B4_4286 0.42 -0.1 4,751,962 + Pf1N1B4_4286 0.42 -2.7 4,751,962 + Pf1N1B4_4286 0.42 -1.6 4,751,962 + Pf1N1B4_4286 0.42 -0.1 4,751,963 - Pf1N1B4_4286 0.42 -0.1 4,751,963 - Pf1N1B4_4286 0.42 -3.6 4,751,963 - Pf1N1B4_4286 0.42 -0.1 4,752,031 + Pf1N1B4_4286 0.47 -0.1 4,752,031 + Pf1N1B4_4286 0.47 -0.1 4,752,031 + Pf1N1B4_4286 0.47 -0.1 4,752,032 - Pf1N1B4_4286 0.47 -0.1 4,752,032 - -0.1 4,752,454 + -0.1 4,752,497 + Pf1N1B4_4286 0.76 -2.0 4,752,498 - Pf1N1B4_4286 0.76 -3.0 4,752,680 - Pf1N1B4_4286 0.88 -1.7 4,752,687 + Pf1N1B4_4286 0.88 -0.1 4,752,687 + Pf1N1B4_4286 0.88 -0.1 4,752,687 + Pf1N1B4_4286 0.88 -2.0 4,752,687 + Pf1N1B4_4286 0.88 -0.1 4,752,687 + Pf1N1B4_4286 0.88 -0.1 4,752,694 + Pf1N1B4_4286 0.89 -1.5 4,752,694 + Pf1N1B4_4286 0.89 -2.0 4,752,750 - -0.1 4,752,914 + -2.0 4,752,914 + -1.8 4,752,914 + -1.6 4,752,915 - -1.6 4,753,005 + +0.1 4,753,005 + -2.3 4,753,005 + -0.1 4,753,426 + Pf1N1B4_4287 0.48 -0.1 4,753,427 - Pf1N1B4_4287 0.48 -0.1 4,753,552 + Pf1N1B4_4287 0.61 -2.3 4,753,553 - Pf1N1B4_4287 0.61 -1.6 4,753,561 + Pf1N1B4_4287 0.62 -0.1 4,753,561 + Pf1N1B4_4287 0.62 -0.1 4,753,561 + Pf1N1B4_4287 0.62 -0.1 4,753,562 - Pf1N1B4_4287 0.62 -2.0 4,753,562 - Pf1N1B4_4287 0.62 -3.0 4,753,562 - Pf1N1B4_4287 0.62 -0.1 4,753,562 - Pf1N1B4_4287 0.62 -0.1 4,753,562 - Pf1N1B4_4287 0.62 -1.6 4,753,568 - Pf1N1B4_4287 0.62 -0.1 4,753,639 + Pf1N1B4_4287 0.69 -0.1 4,753,726 + Pf1N1B4_4287 0.78 -0.1 4,753,727 - Pf1N1B4_4287 0.78 -1.4 4,753,727 - Pf1N1B4_4287 0.78 -0.1 4,753,727 - Pf1N1B4_4287 0.78 -0.1 4,753,846 - Pf1N1B4_4287 0.89 -1.9 4,753,867 + -1.9 4,753,867 + -0.1 4,753,868 - -1.8 4,753,868 - -0.1 4,753,868 - -1.7 4,753,868 - -0.1 4,753,869 + -0.1 4,753,869 + -0.1 4,753,869 + -1.6 4,753,870 - -1.4 4,753,870 - -0.1 4,753,870 - -0.1
Or see this region's nucleotide sequence