Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4176 and Pf1N1B4_4177 are separated by 33 nucleotides Pf1N1B4_4177 and Pf1N1B4_4178 are separated by 167 nucleotides Pf1N1B4_4178 and Pf1N1B4_4179 are separated by 154 nucleotides Pf1N1B4_4179 and Pf1N1B4_4180 are separated by 163 nucleotides
Pf1N1B4_4176 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,624,657 to 4,625,640
_4176
Pf1N1B4_4177 - transcriptional regulator, Crp/Fnr family, at 4,625,674 to 4,626,420
_4177
Pf1N1B4_4178 - Cell division protein FtsH (EC 3.4.24.-), at 4,626,588 to 4,628,375
_4178
Pf1N1B4_4179 - phosphoribosyltransferase, at 4,628,530 to 4,629,114
_4179
Pf1N1B4_4180 - Phosphoribosyl transferase domain protein, at 4,629,278 to 4,629,955
_4180
Position (kb)
4626
4627
4628
4629 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4625.643 kb on + strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.825 kb on + strand, within Pf1N1B4_4177 at 4625.826 kb on - strand, within Pf1N1B4_4177 at 4626.056 kb on + strand, within Pf1N1B4_4177 at 4626.057 kb on - strand, within Pf1N1B4_4177 at 4626.389 kb on + strand at 4626.425 kb on + strand at 4626.453 kb on + strand at 4626.453 kb on + strand at 4626.695 kb on - strand at 4626.754 kb on + strand at 4626.794 kb on - strand, within Pf1N1B4_4178 at 4626.922 kb on + strand, within Pf1N1B4_4178 at 4626.923 kb on - strand, within Pf1N1B4_4178 at 4627.025 kb on + strand, within Pf1N1B4_4178 at 4627.027 kb on + strand, within Pf1N1B4_4178 at 4627.068 kb on - strand, within Pf1N1B4_4178 at 4627.068 kb on - strand, within Pf1N1B4_4178 at 4627.126 kb on + strand, within Pf1N1B4_4178 at 4627.198 kb on - strand, within Pf1N1B4_4178 at 4627.361 kb on + strand, within Pf1N1B4_4178 at 4627.362 kb on - strand at 4627.531 kb on - strand at 4627.531 kb on - strand, within Pf1N1B4_4178 at 4627.834 kb on + strand, within Pf1N1B4_4178 at 4627.834 kb on + strand, within Pf1N1B4_4178 at 4628.328 kb on - strand at 4628.391 kb on + strand at 4628.515 kb on + strand at 4628.516 kb on - strand at 4628.521 kb on + strand at 4628.711 kb on + strand, within Pf1N1B4_4179 at 4628.711 kb on + strand, within Pf1N1B4_4179 at 4628.712 kb on - strand, within Pf1N1B4_4179 at 4628.741 kb on + strand, within Pf1N1B4_4179 at 4628.806 kb on - strand at 4628.885 kb on + strand, within Pf1N1B4_4179 at 4628.953 kb on + strand, within Pf1N1B4_4179 at 4628.954 kb on - strand at 4629.149 kb on + strand at 4629.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,625,643 + -1.9 4,625,644 - -1.2 4,625,644 - -0.0 4,625,644 - -0.0 4,625,644 - -0.0 4,625,825 + Pf1N1B4_4177 0.20 -0.0 4,625,826 - Pf1N1B4_4177 0.20 -0.0 4,626,056 + Pf1N1B4_4177 0.51 -1.9 4,626,057 - Pf1N1B4_4177 0.51 -2.1 4,626,389 + -1.6 4,626,425 + -0.0 4,626,453 + -0.0 4,626,453 + -0.0 4,626,695 - -0.0 4,626,754 + -0.0 4,626,794 - Pf1N1B4_4178 0.12 -2.2 4,626,922 + Pf1N1B4_4178 0.19 -0.0 4,626,923 - Pf1N1B4_4178 0.19 -0.0 4,627,025 + Pf1N1B4_4178 0.24 -3.5 4,627,027 + Pf1N1B4_4178 0.25 -0.0 4,627,068 - Pf1N1B4_4178 0.27 -0.0 4,627,068 - Pf1N1B4_4178 0.27 -0.0 4,627,126 + Pf1N1B4_4178 0.30 -0.0 4,627,198 - Pf1N1B4_4178 0.34 -0.0 4,627,361 + Pf1N1B4_4178 0.43 -0.0 4,627,362 - -0.0 4,627,531 - -1.8 4,627,531 - Pf1N1B4_4178 0.53 -2.8 4,627,834 + Pf1N1B4_4178 0.70 -0.0 4,627,834 + Pf1N1B4_4178 0.70 -1.3 4,628,328 - -2.6 4,628,391 + -0.6 4,628,515 + -0.9 4,628,516 - -0.0 4,628,521 + -0.0 4,628,711 + Pf1N1B4_4179 0.31 -0.0 4,628,711 + Pf1N1B4_4179 0.31 -3.1 4,628,712 - Pf1N1B4_4179 0.31 -0.0 4,628,741 + Pf1N1B4_4179 0.36 -2.8 4,628,806 - -0.2 4,628,885 + Pf1N1B4_4179 0.61 -1.1 4,628,953 + Pf1N1B4_4179 0.72 -0.0 4,628,954 - -1.9 4,629,149 + -0.0 4,629,149 + -0.0
Or see this region's nucleotide sequence