Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4143 and Pf1N1B4_4144 are separated by 224 nucleotides
Pf1N1B4_4143 - hypothetical protein, at 4,588,701 to 4,589,003
_4143
Pf1N1B4_4144 - Mobile element protein, at 4,589,228 to 4,589,341
_4144
Position (kb)
4589
4590 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4588.295 kb on + strand at 4588.296 kb on - strand at 4588.424 kb on + strand at 4588.424 kb on + strand at 4588.425 kb on - strand at 4588.425 kb on - strand at 4588.437 kb on - strand at 4588.464 kb on - strand at 4588.528 kb on + strand at 4588.632 kb on - strand at 4588.675 kb on - strand at 4588.675 kb on - strand at 4588.809 kb on - strand, within Pf1N1B4_4143 at 4588.865 kb on + strand, within Pf1N1B4_4143 at 4588.865 kb on + strand, within Pf1N1B4_4143 at 4588.866 kb on - strand, within Pf1N1B4_4143 at 4588.866 kb on - strand, within Pf1N1B4_4143 at 4588.866 kb on - strand at 4588.926 kb on + strand, within Pf1N1B4_4143 at 4588.979 kb on - strand at 4589.053 kb on + strand at 4589.054 kb on - strand at 4589.054 kb on - strand at 4589.058 kb on - strand at 4589.058 kb on - strand at 4589.058 kb on - strand at 4589.058 kb on - strand at 4589.058 kb on - strand at 4589.069 kb on - strand at 4589.168 kb on + strand at 4589.168 kb on + strand at 4589.169 kb on - strand at 4589.198 kb on + strand at 4589.199 kb on - strand at 4589.248 kb on + strand, within Pf1N1B4_4144 at 4589.339 kb on + strand at 4589.433 kb on + strand at 4589.433 kb on + strand at 4589.433 kb on + strand at 4589.434 kb on - strand at 4589.528 kb on + strand at 4589.566 kb on + strand at 4589.567 kb on - strand at 4589.678 kb on - strand at 4589.721 kb on + strand at 4589.839 kb on - strand at 4589.870 kb on - strand at 4589.896 kb on + strand at 4589.896 kb on + strand at 4589.897 kb on - strand at 4589.897 kb on - strand at 4589.897 kb on - strand at 4589.900 kb on - strand at 4590.095 kb on + strand at 4590.096 kb on - strand at 4590.096 kb on - strand at 4590.138 kb on - strand at 4590.140 kb on - strand at 4590.230 kb on - strand at 4590.268 kb on + strand at 4590.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,588,295 + +0.0 4,588,296 - -2.1 4,588,424 + +0.0 4,588,424 + +0.0 4,588,425 - +0.0 4,588,425 - -1.5 4,588,437 - +0.0 4,588,464 - +0.0 4,588,528 + +0.0 4,588,632 - +0.0 4,588,675 - +0.0 4,588,675 - -0.9 4,588,809 - Pf1N1B4_4143 0.36 +0.6 4,588,865 + Pf1N1B4_4143 0.54 -1.9 4,588,865 + Pf1N1B4_4143 0.54 +0.0 4,588,866 - Pf1N1B4_4143 0.54 +0.0 4,588,866 - Pf1N1B4_4143 0.54 +0.0 4,588,866 - -1.2 4,588,926 + Pf1N1B4_4143 0.74 -2.1 4,588,979 - +0.0 4,589,053 + +1.0 4,589,054 - +0.0 4,589,054 - +0.0 4,589,058 - +0.0 4,589,058 - -2.0 4,589,058 - +0.0 4,589,058 - +0.0 4,589,058 - -1.3 4,589,069 - +0.0 4,589,168 + +0.0 4,589,168 + +0.0 4,589,169 - +0.0 4,589,198 + +0.0 4,589,199 - -2.0 4,589,248 + Pf1N1B4_4144 0.18 +0.0 4,589,339 + +0.0 4,589,433 + +0.0 4,589,433 + -2.9 4,589,433 + +0.0 4,589,434 - -1.7 4,589,528 + +0.0 4,589,566 + +0.0 4,589,567 - -1.2 4,589,678 - +0.1 4,589,721 + -2.3 4,589,839 - -2.2 4,589,870 - +0.0 4,589,896 + +0.0 4,589,896 + +0.0 4,589,897 - +0.0 4,589,897 - -1.3 4,589,897 - -2.6 4,589,900 - -0.7 4,590,095 + +0.0 4,590,096 - +0.0 4,590,096 - -1.3 4,590,138 - +0.0 4,590,140 - -1.0 4,590,230 - -2.4 4,590,268 + +0.0 4,590,340 - +0.0
Or see this region's nucleotide sequence