Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4101 and Pf1N1B4_4102 are separated by 96 nucleotides Pf1N1B4_4102 and Pf1N1B4_4103 are separated by 284 nucleotides
Pf1N1B4_4101 - Transcriptional regulator, AraC family, at 4,549,784 to 4,550,575
_4101
Pf1N1B4_4102 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 4,550,672 to 4,551,058
_4102
Pf1N1B4_4103 - Flagellar synthesis regulator FleN, at 4,551,343 to 4,552,164
_4103
Position (kb)
4550
4551
4552 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4549.877 kb on - strand, within Pf1N1B4_4101 at 4549.881 kb on - strand, within Pf1N1B4_4101 at 4549.909 kb on - strand, within Pf1N1B4_4101 at 4550.033 kb on + strand, within Pf1N1B4_4101 at 4550.033 kb on + strand, within Pf1N1B4_4101 at 4550.034 kb on - strand, within Pf1N1B4_4101 at 4550.061 kb on + strand, within Pf1N1B4_4101 at 4550.062 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand at 4550.121 kb on - strand, within Pf1N1B4_4101 at 4550.381 kb on + strand, within Pf1N1B4_4101 at 4550.382 kb on - strand, within Pf1N1B4_4101 at 4550.387 kb on - strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.590 kb on + strand at 4550.630 kb on + strand at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.979 kb on + strand, within Pf1N1B4_4102 at 4551.220 kb on + strand at 4551.461 kb on + strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.499 kb on - strand, within Pf1N1B4_4103 at 4551.653 kb on - strand at 4551.675 kb on + strand, within Pf1N1B4_4103
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,549,877 - Pf1N1B4_4101 0.12 -1.2 4,549,881 - Pf1N1B4_4101 0.12 -1.4 4,549,909 - Pf1N1B4_4101 0.16 -0.0 4,550,033 + Pf1N1B4_4101 0.31 -0.0 4,550,033 + Pf1N1B4_4101 0.31 -0.0 4,550,034 - Pf1N1B4_4101 0.32 -1.2 4,550,061 + Pf1N1B4_4101 0.35 -1.4 4,550,062 - Pf1N1B4_4101 0.35 -1.0 4,550,099 - -0.0 4,550,099 - Pf1N1B4_4101 0.40 -0.6 4,550,099 - Pf1N1B4_4101 0.40 -1.5 4,550,099 - Pf1N1B4_4101 0.40 -1.2 4,550,099 - Pf1N1B4_4101 0.40 -0.0 4,550,099 - -1.4 4,550,121 - Pf1N1B4_4101 0.43 -3.0 4,550,381 + Pf1N1B4_4101 0.75 -0.0 4,550,382 - Pf1N1B4_4101 0.76 -1.5 4,550,387 - Pf1N1B4_4101 0.76 -1.1 4,550,402 + Pf1N1B4_4101 0.78 -0.0 4,550,402 + Pf1N1B4_4101 0.78 -0.6 4,550,402 + Pf1N1B4_4101 0.78 -1.3 4,550,403 - Pf1N1B4_4101 0.78 -0.9 4,550,403 - Pf1N1B4_4101 0.78 -0.0 4,550,590 + -0.0 4,550,630 + -0.0 4,550,921 + Pf1N1B4_4102 0.64 -1.7 4,550,921 + Pf1N1B4_4102 0.64 -0.0 4,550,921 + Pf1N1B4_4102 0.64 -2.8 4,550,922 - Pf1N1B4_4102 0.65 -0.0 4,550,922 - Pf1N1B4_4102 0.65 -0.0 4,550,979 + Pf1N1B4_4102 0.79 -0.0 4,551,220 + -2.2 4,551,461 + Pf1N1B4_4103 0.14 -0.0 4,551,462 - Pf1N1B4_4103 0.14 -0.0 4,551,462 - Pf1N1B4_4103 0.14 -0.6 4,551,499 - Pf1N1B4_4103 0.19 -1.0 4,551,653 - -1.0 4,551,675 + Pf1N1B4_4103 0.40 -0.0
Or see this region's nucleotide sequence