Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides Pf1N1B4_4051 and Pf1N1B4_4052 are separated by 493 nucleotides 
        Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589 
        _4048 
         
        
        Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095 
        _4049 
         
        
        Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422 
        _4050 
         
        
        Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533 
        _4051 
         
        
        Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283 
        _4052 
         Position (kb) 4502 
4503 
4504 
4505 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand at 4502.663 kb on - strand at 4502.673 kb on + strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.678 kb on - strand, within Pf1N1B4_4051 at 4503.004 kb on - strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.389 kb on + strand, within Pf1N1B4_4051 at 4503.566 kb on + strand, within Pf1N1B4_4051 at 4503.625 kb on - strand, within Pf1N1B4_4051 at 4503.710 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.506 kb on + strand at 4504.506 kb on + strand at 4504.508 kb on + strand at 4504.508 kb on + strand at 4504.560 kb on + strand at 4504.692 kb on - strand at 4504.727 kb on + strand at 4504.769 kb on + strand at 4504.770 kb on - strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.780 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.856 kb on + strand at 4504.856 kb on + strand at 4504.910 kb on + strand at 4504.910 kb on + strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.953 kb on - strand at 4504.971 kb on + strand at 4504.972 kb on - strand at 4504.972 kb on - strand at 4504.981 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.140 kb on + strand at 4505.141 kb on - strand at 4505.153 kb on - strand, within Pf1N1B4_4052 at 4505.237 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand at 4505.320 kb on - strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.511 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,501,709 -  -1.5 4,501,709 -  Pf1N1B4_4049 0.13  -0.9 4,501,806 +  Pf1N1B4_4049 0.35  +0.0 4,501,854 +  Pf1N1B4_4049 0.46  -1.2 4,501,854 +  Pf1N1B4_4049 0.46  +0.0 4,501,855 -  Pf1N1B4_4049 0.46  -1.2 4,501,881 +  Pf1N1B4_4049 0.52  +0.0 4,501,881 +  Pf1N1B4_4049 0.52  +0.0 4,501,881 +  Pf1N1B4_4049 0.52  -1.4 4,501,887 +  -3.4 4,501,887 +  Pf1N1B4_4049 0.53  +0.0 4,501,887 +  Pf1N1B4_4049 0.53  -2.0 4,501,887 +  Pf1N1B4_4049 0.53  +0.0 4,501,887 +  Pf1N1B4_4049 0.53  -0.7 4,502,239 +  -2.4 4,502,239 +  -1.7 4,502,240 -  -1.7 4,502,240 -  -0.6 4,502,240 -  -1.0 4,502,240 -  -3.0 4,502,240 -  -0.9 4,502,240 -  +0.0 4,502,240 -  +0.0 4,502,240 -  +0.0 4,502,240 -  -0.8 4,502,240 -  +0.0 4,502,240 -  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  -1.0 4,502,243 +  -2.3 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  +0.0 4,502,243 +  -1.0 4,502,243 +  +0.0 4,502,244 -  +0.0 4,502,244 -  -1.1 4,502,244 -  +0.0 4,502,244 -  +0.0 4,502,244 -  -0.3 4,502,247 +  +0.0 4,502,247 +  +0.0 4,502,247 +  -1.7 4,502,247 +  +0.0 4,502,248 -  -2.8 4,502,248 -  -2.0 4,502,302 +  +0.0 4,502,310 +  -1.3 4,502,551 -  +0.0 4,502,663 -  +0.0 4,502,673 +  Pf1N1B4_4051 0.10  -1.6 4,502,674 -  Pf1N1B4_4051 0.10  -2.0 4,502,674 -  Pf1N1B4_4051 0.10  +0.0 4,502,678 -  Pf1N1B4_4051 0.10  +0.0 4,503,004 -  Pf1N1B4_4051 0.26  +0.0 4,503,020 +  Pf1N1B4_4051 0.27  +0.0 4,503,020 +  Pf1N1B4_4051 0.27  +0.0 4,503,389 +  Pf1N1B4_4051 0.45  -1.0 4,503,566 +  Pf1N1B4_4051 0.53  -2.0 4,503,625 -  Pf1N1B4_4051 0.56  -2.8 4,503,710 +  Pf1N1B4_4051 0.60  +1.0 4,504,073 +  Pf1N1B4_4051 0.78  +0.0 4,504,073 +  Pf1N1B4_4051 0.78  +0.0 4,504,472 +  -2.5 4,504,472 +  +0.0 4,504,472 +  +0.0 4,504,506 +  -1.8 4,504,506 +  -3.0 4,504,508 +  -0.3 4,504,508 +  -1.0 4,504,560 +  +0.0 4,504,692 -  +0.0 4,504,727 +  -1.5 4,504,769 +  +0.0 4,504,770 -  -1.6 4,504,771 +  +0.0 4,504,771 +  +0.0 4,504,771 +  +0.0 4,504,771 +  -0.3 4,504,771 +  +0.0 4,504,771 +  -1.1 4,504,771 +  -2.3 4,504,771 +  +0.0 4,504,771 +  +0.0 4,504,771 +  -1.1 4,504,771 +  +0.0 4,504,771 +  -0.8 4,504,771 +  +0.0 4,504,771 +  -1.8 4,504,771 +  -1.7 4,504,771 +  +0.0 4,504,771 +  +0.0 4,504,772 -  +0.0 4,504,772 -  -1.1 4,504,772 -  -1.1 4,504,772 -  +0.0 4,504,772 -  +0.0 4,504,772 -  +0.0 4,504,775 +  -2.5 4,504,775 +  +0.0 4,504,775 +  +0.0 4,504,775 +  -1.5 4,504,775 +  +0.0 4,504,775 +  -1.6 4,504,775 +  -1.3 4,504,775 +  -0.9 4,504,776 -  +0.0 4,504,776 -  +0.0 4,504,776 -  -3.1 4,504,776 -  +0.0 4,504,776 -  -3.5 4,504,776 -  +0.0 4,504,776 -  -2.2 4,504,780 -  +0.0 4,504,805 -  -3.2 4,504,805 -  +0.0 4,504,805 -  +0.0 4,504,830 +  +0.0 4,504,830 +  +0.0 4,504,830 +  +0.0 4,504,856 +  -2.5 4,504,856 +  -2.8 4,504,910 +  -3.4 4,504,910 +  -1.4 4,504,911 -  -3.8 4,504,911 -  -2.5 4,504,911 -  -1.8 4,504,911 -  -1.5 4,504,911 -  -0.6 4,504,911 -  -2.2 4,504,952 +  +0.0 4,504,952 +  +0.0 4,504,952 +  +0.0 4,504,952 +  +0.0 4,504,952 +  +0.0 4,504,953 -  -1.4 4,504,971 +  +0.0 4,504,972 -  -3.9 4,504,972 -  +0.4 4,504,981 -  +0.0 4,505,022 -  +0.0 4,505,022 -  -2.2 4,505,022 -  +0.0 4,505,140 +  -2.1 4,505,141 -  -1.9 4,505,153 -  Pf1N1B4_4052 0.10  +0.0 4,505,237 +  Pf1N1B4_4052 0.17  -1.3 4,505,315 +  Pf1N1B4_4052 0.23  +0.0 4,505,315 +  Pf1N1B4_4052 0.23  -1.4 4,505,315 +  Pf1N1B4_4052 0.23  -1.8 4,505,315 +  Pf1N1B4_4052 0.23  +0.0 4,505,316 -  Pf1N1B4_4052 0.23  +0.0 4,505,316 -  Pf1N1B4_4052 0.23  -1.8 4,505,319 +  Pf1N1B4_4052 0.23  +0.0 4,505,319 +  Pf1N1B4_4052 0.23  -2.4 4,505,319 +  -1.6 4,505,320 -  Pf1N1B4_4052 0.23  +0.0 4,505,405 +  Pf1N1B4_4052 0.30  +0.0 4,505,405 +  Pf1N1B4_4052 0.30  +0.0 4,505,405 +  Pf1N1B4_4052 0.30  +0.0 4,505,405 +  Pf1N1B4_4052 0.30  +0.0 4,505,405 +  Pf1N1B4_4052 0.30  -2.8 4,505,405 +  -1.0 4,505,406 -  Pf1N1B4_4052 0.30  +0.0 4,505,406 -  Pf1N1B4_4052 0.30  +0.0 4,505,510 +  Pf1N1B4_4052 0.38  +0.0 4,505,510 +  Pf1N1B4_4052 0.38  -3.5 4,505,510 +  Pf1N1B4_4052 0.38  +0.0 4,505,511 -  -0.8 
Or see this region's nucleotide sequence