Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3932 and Pf1N1B4_3933 are separated by 140 nucleotides Pf1N1B4_3933 and Pf1N1B4_3934 are separated by 103 nucleotides Pf1N1B4_3934 and Pf1N1B4_3935 are separated by 335 nucleotides
Pf1N1B4_3932 - Virulence factor mviM, at 4,369,242 to 4,369,889
_3932
Pf1N1B4_3933 - FIG00956224: hypothetical protein, at 4,370,030 to 4,370,539
_3933
Pf1N1B4_3934 - Sodium:dicarboxylate symporter, at 4,370,643 to 4,371,875
_3934
Pf1N1B4_3935 - Major facilitator family transporter, at 4,372,211 to 4,373,365
_3935
Position (kb)
4370
4371
4372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4369.934 kb on - strand at 4369.938 kb on + strand at 4369.939 kb on - strand at 4369.985 kb on + strand at 4369.985 kb on + strand at 4369.986 kb on - strand at 4369.986 kb on - strand at 4369.986 kb on - strand at 4369.987 kb on + strand at 4369.987 kb on + strand at 4370.005 kb on - strand at 4370.035 kb on + strand at 4370.036 kb on - strand at 4370.036 kb on - strand at 4370.036 kb on - strand at 4370.046 kb on - strand at 4370.264 kb on + strand, within Pf1N1B4_3933 at 4370.426 kb on + strand, within Pf1N1B4_3933 at 4370.439 kb on - strand, within Pf1N1B4_3933 at 4370.439 kb on - strand at 4370.439 kb on - strand at 4370.522 kb on + strand at 4370.522 kb on + strand at 4370.522 kb on + strand at 4370.522 kb on + strand at 4370.523 kb on - strand at 4370.537 kb on + strand at 4370.572 kb on + strand at 4370.606 kb on + strand at 4370.607 kb on - strand at 4370.631 kb on + strand at 4370.632 kb on - strand at 4370.931 kb on - strand, within Pf1N1B4_3934 at 4371.016 kb on + strand, within Pf1N1B4_3934 at 4371.016 kb on + strand, within Pf1N1B4_3934 at 4371.069 kb on - strand, within Pf1N1B4_3934 at 4371.083 kb on + strand at 4371.084 kb on - strand, within Pf1N1B4_3934 at 4371.236 kb on - strand, within Pf1N1B4_3934 at 4371.236 kb on - strand, within Pf1N1B4_3934 at 4371.361 kb on - strand, within Pf1N1B4_3934 at 4371.361 kb on - strand, within Pf1N1B4_3934 at 4371.361 kb on - strand, within Pf1N1B4_3934 at 4371.528 kb on + strand, within Pf1N1B4_3934 at 4371.528 kb on + strand, within Pf1N1B4_3934 at 4371.570 kb on - strand, within Pf1N1B4_3934 at 4371.617 kb on - strand, within Pf1N1B4_3934 at 4371.617 kb on - strand, within Pf1N1B4_3934 at 4371.893 kb on + strand at 4371.897 kb on - strand at 4371.968 kb on + strand at 4371.969 kb on - strand at 4372.004 kb on + strand at 4372.004 kb on + strand at 4372.004 kb on + strand at 4372.005 kb on - strand at 4372.005 kb on - strand at 4372.130 kb on - strand at 4372.157 kb on - strand at 4372.391 kb on + strand, within Pf1N1B4_3935 at 4372.581 kb on - strand, within Pf1N1B4_3935 at 4372.735 kb on + strand at 4372.735 kb on + strand at 4372.736 kb on - strand, within Pf1N1B4_3935 at 4372.740 kb on - strand at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.875 kb on - strand, within Pf1N1B4_3935
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,369,934 - +0.0 4,369,938 + -0.9 4,369,939 - -1.4 4,369,985 + +0.0 4,369,985 + +0.0 4,369,986 - +0.0 4,369,986 - +0.0 4,369,986 - +0.0 4,369,987 + +0.0 4,369,987 + +0.0 4,370,005 - +1.0 4,370,035 + -0.6 4,370,036 - -1.8 4,370,036 - +0.0 4,370,036 - +0.0 4,370,046 - -2.0 4,370,264 + Pf1N1B4_3933 0.46 +0.0 4,370,426 + Pf1N1B4_3933 0.78 +0.0 4,370,439 - Pf1N1B4_3933 0.80 +0.0 4,370,439 - +0.0 4,370,439 - +0.0 4,370,522 + +0.0 4,370,522 + -1.0 4,370,522 + -2.3 4,370,522 + +0.0 4,370,523 - -1.7 4,370,537 + +0.0 4,370,572 + +0.0 4,370,606 + -0.3 4,370,607 - +0.0 4,370,631 + -1.0 4,370,632 - +0.0 4,370,931 - Pf1N1B4_3934 0.23 +0.0 4,371,016 + Pf1N1B4_3934 0.30 -2.0 4,371,016 + Pf1N1B4_3934 0.30 +0.0 4,371,069 - Pf1N1B4_3934 0.35 +0.0 4,371,083 + -1.5 4,371,084 - Pf1N1B4_3934 0.36 +0.0 4,371,236 - Pf1N1B4_3934 0.48 -1.9 4,371,236 - Pf1N1B4_3934 0.48 +2.0 4,371,361 - Pf1N1B4_3934 0.58 +0.0 4,371,361 - Pf1N1B4_3934 0.58 +0.0 4,371,361 - Pf1N1B4_3934 0.58 +0.0 4,371,528 + Pf1N1B4_3934 0.72 -2.1 4,371,528 + Pf1N1B4_3934 0.72 +0.0 4,371,570 - Pf1N1B4_3934 0.75 -2.1 4,371,617 - Pf1N1B4_3934 0.79 +0.0 4,371,617 - Pf1N1B4_3934 0.79 -3.7 4,371,893 + +0.0 4,371,897 - +0.0 4,371,968 + +0.0 4,371,969 - -0.2 4,372,004 + +0.0 4,372,004 + +0.5 4,372,004 + +0.0 4,372,005 - -1.6 4,372,005 - -1.9 4,372,130 - -2.0 4,372,157 - -0.9 4,372,391 + Pf1N1B4_3935 0.16 +0.0 4,372,581 - Pf1N1B4_3935 0.32 -1.4 4,372,735 + +0.0 4,372,735 + +0.0 4,372,736 - Pf1N1B4_3935 0.45 -0.4 4,372,740 - +0.0 4,372,874 + Pf1N1B4_3935 0.57 +0.0 4,372,874 + Pf1N1B4_3935 0.57 -1.6 4,372,874 + Pf1N1B4_3935 0.57 -1.8 4,372,875 - Pf1N1B4_3935 0.57 +0.0
Or see this region's nucleotide sequence